Do Now: 3/16 Period 4: Science & Computers 3 rd period today – get right to work! Period 2: Radiation levels in Tokyo are 20 times above background levels,

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Do Now: 3/16 Period 4: Science & Computers 3 rd period today – get right to work! Period 2: Radiation levels in Tokyo are 20 times above background levels, with recent measurements about 10 μSv/day. Remember the 1% / 5 year cancer threshold we talked about yesterday is 1 Sv. 1. The Tokyo Metropolitan Government has assured residents of Japan’s capital city that the area is still safe. Do you agree? Justify your answer mSv/hr is considered to be the level “dangerous to human health.” How many hours of exposure at this level will produce the 1% cancer effect?

Syllabus Update There is NO CHANGE to the assigned readings. GEL ELECTROPHORESIS is now today’s topic, because of our “breaking science news” day yesterday. GENOMICS / BIOINFORMATICS will no longer be a topic in class. There is NO CHANGE to the assigned readings.

GEL ELECTROPHORESIS WHAT IT IS: A method for separating DNA fragments based on their size. WHY IT’S USEFULL: – Isolating important sequences from others – Measuring & mapping DNA sequences – Identification (DNA fingerprinting) HOW IT WORKS: Different sized DNA molecules move through a gel at different speeds, causing them to separate.

Virtual Lab el/

Short fragments of DNA move faster through gel

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Plasmid Mapping Extra Credit You have been given gel electrophoresis data from the digestion of a fictitious plasmid, pOLY1, with several restriction enzymes. Each enzyme cuts the plasmid in one or two locations. Use the fragment sizes to make a map that shows how the restriction sites are arranged on the plasmid. Indicate the distance between sites in kbp.