Rhizobium Activity Manhasset Science Research. Need Bean Yellow Mosaic Virus- transmitted by aphids- eat nitrogen fixation nodules: malformation of leaves.

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Presentation transcript:

Rhizobium Activity Manhasset Science Research

Need Bean Yellow Mosaic Virus- transmitted by aphids- eat nitrogen fixation nodules: malformation of leaves (Brunt, 1996) -bean crops had an average within crop incidence of BYMV of 26% of plants, ranging from 1-63% (Van Leur, 2002) -transmitted by vector (insect); not transmitted by mechanical inoculation, by seed, or by pollen (Brunt 1996)

Knowledge Base (Deacon, 2009) Nodules are formed on bean stems and leaves, allowing nitrogen fixation to occur, stimulating plant growth (Burdass, 2002) Aphids are classified as sap- sucking, soft-bodied insects (Homoptera), transmitting diseases including BYMV (Day, 1996) (Clark, 1998)

Knowledge Base Rhizobium is the centrally most important and well-known species of a bacterial group acting as the chief symbiotic nitrogen fixer (Burdass, 2002) Poole, 2006 González, 2006 Preliminary interaction between the host plant and free-living rhizobia is the release of a variety of different chemicals by the root cells from the plant into the surrounding soil, causing nodule formation (Burdass, 2002)

Literature Review González, has done research on the role of exopolysaccharides in legumes and nodulation by rhizobia and the molecular genetics of plant-microbe interactions -work suggests that the mode of regulation and quorum sensing, intercedes nitrogen-fixation processes Webster, work done involves an enzyme linked immuno-sorbent assay (ELISA), using general Potyvirus antiserum and amplification by reverse transcription polymerase chain reaction (RT-PCR) with degenerate primers indicated that it was a species of Potyvirus -attempt through biological process to find reasoning for BYMV in Western Australia and Eastern Asia

Citations Brunt, A.A. “Mung Bean Yellow Mosaic Bigeminivirus.” Plant Viruses Online Descriptions and Lists from the VIDE Database. 20 Aug Van Leur, J. “Temperate Pulse Viruses: Bean Yellow Mosaic Virus (BYMV).” DEPARTMENTOF PRIMARY INDUSTRIES Symptoms/Economic Importance. 2-7 Feb C871BE2A A2568B30004D413-3C5B474B480825C8CA256BC F1BBD6496F89034A256DEA00274EEC-494B7E2886B7BE85CA C1?open 22C871BE2A A2568B30004D413-3C5B474B480825C8CA256BC F1BBD6496F89034A256DEA00274EEC-494B7E2886B7BE85CA C1?open Burdass, Dariel. “Rhizobium, Root Nodules & Nitrogen Fixation.” Society for general Microbiology Microbiology Online Day, Eric. “Aphid.” Virginia Cooperative Extension Virginia State University. Aug González, Juan E. “Faculty and Research.” Department of Molecular & Cell Biology Research Interests. 6 Feb Webster, Craig Graham. “Characterisation of Hardenbergia Mosaic Virus and Development of Microarrays for Detecting Viruses in Plants.” Murdoch Research Repository Murdoch University