1. Future Studies Use other leguminous plants that produce food: –Beans Chemically analyze seeds Determine the effects on the nutritional value of plants Different concentrations of solution could be used Bibliography Bhuvaneswari, T. V., et. al. "Transient Susceptibility of Root Cells in Four Common Legumes to Nodulation by Rhizobia." Plant Physiology 68 (1981): 1144-149. Bloemberg, Guido V., and Ben J. Lugtenberg. "Molecular basis of plant growth promotion and biocontrol by rhizobacteria." Current Opinion in Plant Biology 4 (2001): 343-50. Elhuyar Fundazioa. "Drought Reduces Nitrogen-fixing In Legumes." ScienceDaily 4 February 2005. 3 November 2008. El-Sheekh, M. M. "Effect of cobalt on growth, pigments and the photosynthetic electron transport in Monoraphidium minutum and Nitzchia perminuta." Brazilian Journal of Plant Physiology 15 (2003): 159-66. Gad, Nadia, and M. A. Atta-Aly. "Effect of Cobalt on the Formation, Growth and Development of Adventitious Roots in Tomato and Cucumber Cuttings." Journal of Applied Sciences Research 2 (2006): 423-29. Gad, Nadia. "Increasing the Efficiency of Nitrogen Fertilization Through Cobalt Application to Pea Plant." Research Journal of Agriculture and Biological Sciences 2 (2005): 433-42. Gaunt, John K., and Bruce B. Stowe. "Analysis and Distribution of Tocopherols and Quinones in the Pea Plant." Plant Physiology 42 (1967): 851-58. Kandil, Hala. "Effect of Cobalt Fertilizer on Growth, Yield and Nutrients Status of Faba Bean (Vicia faba L.) Plants." Journal of Applied Sciences Research 3 (2007): 867-72. Kliewer, Mark, and Harold J. Evans. "Identification of Cobamide Coenzyme in Nodules of Symbionts & Isolation of the B12 Coenzyme From Rhizobium meliloti." Plant Physiology 38 (1963): 55-59. Martens, J. H. et. al. "Microbial production of vitamin B12." Applied microbiology and biotechnology 58 (2002): 275-85. Miller, Carlos O. "The Influence of Cobalt and Sugars upon the Elongation of Etiolated Pea Stem Segments." Plant Physiology 29 (1954): 79-82. Miller, Richard E., and Robert T. Tarrant. "Long-term Growth Response of Douglas-fir to Ammonium Nitrate Fertilizer." Annals of Forest Science 29 (1983): 127-37. Mylona, Panagiota, et. al. "Symbiotic Nitrogen Fixation." The Plant Cell 7 (1995): 869- 85. Sandalio, L. M., and Et al. "Cadmium-induced Changes in the Growht and Oxidative Metabolism of Pea Plants." Journal of Experimental Biology 52 (2001): 2115-126. Zhang, Feng, et al. "Plant Growth Promoting Rhizobacteria and Soybean [Glycine max (L.) Merr.] Nodulation and Nitrogen Fixation at Suboptimal Root Zone Temperatures." Annals of Botany 77 (1996): 453-59 Discussion Conclusion The data demonstrates that cobalt sulfate and vitamin B12 were both significantly more effective than water. The presence of rhizobium did not affect plant growth. No nodules were found, no data collected. The data also demonstrates that water significantly raised germination rates for the plants. This supports the work of Gad and Kandil. Limitations –Vitamin B12 had an outlier in each trial –Human error when measuring and planting seeds –Germination rates affected the overall mean growth Cobalt Sulfate was just as effective as Vitamin B12 in stimulating plant growth. Both Cobalt Sulfate and Vitamin B12 lowered germination rates for the plants. Cobalt Sulfate is unhealthy for the microorganisms that live in the soil. The data supports the alternate hypothesis. http://plants.usda.gov/java/profile?symbol=pisa6 http://www.energyfarms.net/files/images/peas.jpg