Microscopes Compound Bright-Field Light Microscope

Slides:

Advertisements

Similar presentations

Adjusting a Microscope 1Center components on optic axis 2Focus objective 3Focus condenser 4Adjust illumination lamp voltage (intensity) iris diaphragm.

Advertisements

Chapter 4 Notes Part II: Microscopy (refer to pg.60-61)

Observing Microorganisms Through a Microscope

Observing Microorganisms Through A Microscope

Microscopy Lecture I.

Chapter 3: Observing Microorganisms Through a Microscope

Laboratory Tools in Microbiology

Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings 1 Chapter 3 Observing Microorganisms Through a Microscope.

Microscopy: The Instruments

Observing Microorganisms Through a Microscope

Microscopy, Staining, and Classification

USE AND CARE OF THE MICROSCOPE LECTURE 1. MICROSCOPY u Light Microscopy: any microscope that uses visible light to observe specimens u Compound Light.

MICROSCOPES Light (visible) Fluorescent U-V Electron Monocular

Microscopy Techniques for Biomaterial Characterization: A Primer Prabhas V. Moghe Lecture 3 September 21, 1999 RU CBE 533 or BME 553; NJIT BME 698.

Introduction to Microscopy. Objectives Learn to use a compound microscope correctly. Diagram the path of light through a compound microscope. Name major.

Copyright © 2010 Pearson Education, Inc. Learning Objectives Observing Microorganisms Through a Microscope Chapter 3.

BIODIVERSITY I BIOL1051 Microscopy Professor Marc C. Lavoie

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C R.

Microscopy. Scale Lenses and the Bending of Light light is refracted (bent) when passing from one medium to another refractive index –a measure of how.

Tools of the Laboratory: The Microscope

Observing Microorganisms Through Microscopes

Honors Microbiology: Chapter 3 Microscopy and Staining

Cycling of Matter in Living Systems 1.3 Developments in Imaging Technology and Staining Techniques.

MICROSCOPY AND STAINING CHAPTER 3. 2 Metric Units.

Microscopy 1. UNITS OF MEASUREMENT 1 m = 1000 mm (millimeters) 1 m = 1000 mm (millimeters) 1000 mm = 1 µm (microns) 1000 mm = 1 µm (microns) Bacteria.

By: Rob Page and Tara Trovarello

Advanced Biology Visualizing Cells. The Human Eye  Resolution – The minimum distance two points can be apart and still be distinguished as two separate.

MICROSCOPY AND STAINING. Leeuwenhoek’s Investigation 2.

A simple microscope has only one lens. Chapter 3 - Microscopy.

Microscopy as an analytical tool Dr Imran. Wavelength and resolution Resolution (r) = λ / (2NA) R= resolution λ = wavelength of light used for imaging.

Copyright © 2010 Pearson Education, Inc. Lectures prepared by Christine L. Case Chapter 3 Observing Microorganisms Through A Microscope.

An instrument for magnifying very small objects

Topic 1 Microscopes.

microscopy There are three well-known branches of microscopy:

1 Metric Units in Microbiology Micrometer (µm) m Nanometer (nm) m.

1 Parts of the Microscope Ocular (eyepiece) Interpupillary adjustment Body Arm Coarse adjustment knob Fine focus adjustment knob Stage adjustment knobs.

LOGO 미생물학실험 Microbiology Laboratory 생물환경학과 김정호.  현미경의 3 주요 부분  Light source ( 광원 )  Lens  Condenser ( 집광기 )  현미경의 3 주요 기능  Magnification ( 배율 )

 Bright-field  Dark-field  Phase Contrast  Fluorescence.

Microscopy Dr. Bhavesh Patel Principal V.P. and R.P.T.P. Science College Vallabh Vidyanagar –

Chapter 3: Microscopic observation of microorganisms

 현미경의 3 주요 부분  Light source  Lens  Condenser ( 집광기 )  현미경의 3 주요 기능  Magnification ( 배율 )  Resolution ( 해상도, 분해능 )  Contrast ( 대비력 )  Light microscope.

Topic 1 Microscopes and Microscopy. Light Microscopes  How do they work?  Optical magnification  Images pass through a lens or a series of lenses 

Unit 1 – Introduction to Microscopy. I. Microscopes A. Proper Care 1.)Expensive – Carry with 2 hands 2.)Keep Glass parts clean! 3.)Never use Coarse adjustment.

Chapter 2: Viewing the Microbial World

Observing Microorganisms Through a Microscope

Tools of the Laboratory: The Methods for Studying Microorganisms

Microbiology: A Systems Approach

Types of Microscopes Light Electron Bright field Dark-field Scanning

Observing Microorganisms Through a Microscope

The Study of Microbial Structure: Microscopy and Specimen Preparation

Microbiology Lab Practices.

4 Microscopy, Staining, and Classification.

A C B D 1. Read the information about different sorts of microscope.

LIGHT MICROSCOPY basic

LIGHT MICROSCOPY variations

Types of Microscopes Light Electron Bright field Dark-field Scanning

Laboratory Exercise 2 “Microscopy”.

The Tools of Microbiology

Basic Microscopy SC.912.L Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom.

140MIC: Microbiology Lecture-6 Microscopes.

Observing Microorganisms Through a Microscope

Microscopes Chapter 7.

Basic Microscopy SC.912.L Compare and contrast structure and function of various types of microscopes. From the Virtual Microbiology Classroom.

Presentation transcript:

Microscopes Compound Bright-Field Light Microscope Maximum Useful Magnification = 1200X Why?

Resolution Useful magnification is limited by Resolution Resolution is influenced by Numerical Aperture (NA) NA determined by Wavelength of Light and Refractive Index Improve Magnification Shorten Wavelength Increase Refractive Index

Staining Simple Stains Light Microscope - Colored Dye Electron Microscope - Electron Dense Differential Stains Gram Stain

Gram Stain Most Important Stain Key Bacteriological Test Conventional Stain Multiple Steps & Subjective Fluorescent Stain One Step & +/-

Optical Tricks Oil Immersion Lens Dark Field Microscope

Phase Contrast Microscopes Other Microscopes Phase Contrast Microscopes

Fluorescent Microscopes

More Microscopes Interference Contrast Microscope Atomic Force Microscope

Confocal Scanning Laser Microscope

Electron Microscopes Shorten wavelength Scanning Electron Microscope- SEM 3 Dimensional images

Transmission Electron Microscope TEM Better Resolution

Microscopes Word List Compound Staining Confocal Light Simple Scanning Differential Laser Bright-Field Gram Stain Electron Magnification Phase Resolution Contrast Transmission Numerical Dark-field Aperture Fluorescent Oil Interference Immersion