Chapter 9 DNA Separation Methods: Slab-Gel and Capillary Electrophoresis ©2002 Academic Press.

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Presentation transcript:

Chapter 9 DNA Separation Methods: Slab-Gel and Capillary Electrophoresis ©2002 Academic Press

Gel Electrophoresis System - Voltage Gel Loading well + anode cathode Side viewTop view Gel lanes DNA bands Buffer + - ©2002 Academic Press

Capillary Electrophoresis System Laser Inlet Buffer Capillary filled with polymer solution 5-20 kV -+ Outlet Buffer Sample tray Detection window (cathode) (anode) Data Acquisition Sample tray moves automatically beneath the cathode end of the capillary to deliver each sample in succession ©2002 Academic Press

DNA Separation by Electrophoresis General Principle Larger DNA molecules interact more frequently with the gel and are thus retarded in their migration through the gel Gel ©2002 Academic Press

DNA Separation Mechanisms Ogston SievingReptation Small DNA molecules Long DNA molecules Gel ©2002 Academic Press