Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 6, part B Microbial Growth

Deep-freezing: -50°to -95°C Lyophilization (freeze-drying): Frozen (- 54° to -72°C) and dehydrated in a vacuum Preserving Bacteria Cultures

Binary fission Budding Conidiospores (actinomycetes) Fragmentation of filaments Reproduction in Prokaryotes

Binary Fission Figure 6.11

Figure 6.12b

If 100 cells growing for 5 hours produced 1,720,320 cells:

Figure 6.13

Figure 6.14

Plate Counts: Perform serial dilutions of a sample Direct Measurements of Microbial Growth Figure 6.15, top portion

Inoculate Petri plates from serial dilutions Plate Count Figure 6.16

After incubation, count colonies on plates that have colonies (CFUs) Plate Count Figure 6.15

Filtration Direct Measurements of Microbial Growth Figure 6.17a, b

Multiple tube MPN test Count positive tubes and compare to statistical MPN table. Direct Measurements of Microbial Growth Figure 6.18b

Direct Measurements of Microbial Growth Direct Microscopic Count

Direct Measurements of Microbial Growth Figure 6.19

Turbidity Estimating Bacterial Numbers by Indirect Methods Figure 620

Estimating Bacterial Numbers by Indirect methods Metabolic activity Dry weight