Prion Digestion in the Alimentary Tract Nicholas Toney.

Slides:

Advertisements

Similar presentations

Distinctive characteristics

Advertisements

® Bovine Derived Products are Safe TSE Advisory Committee Serologicals Corporation February 2004.

Growth and Development of the Ruminant Gastro-Intestinal Tract   GI Tract development begins very early in gestation.   Ruminant enters life as a.

Viral Detection By: Douglas Tran.  Past research  States Prion hypothesis and past research of no past viral genetic material detected  States the.

PRIONS Defn: small proteinaceous infectious particles that resist inactivation by procedures that modify viruses and nucleic acids.

Mouse Prion Protein Domain PrP( ) Andreas Razen Geometric Computations in Molecular Biology 2 May 2007.

Bovine Spongiform Encephalopathy Luke VanNatter Carrie Pell Amy Richwine Scott Inskeep Kristina Anderson.

Prions Fact or Science Fiction?. Stanley Prusiner, 1982 Born in Des Moines, Ia. Suggested that spongiform encephalopathies in animals and humans are caused.

L and D isomers of amino acids. Ionization state as a function of pH.

Prions Alicia Arguelles, Jerry Wang May 4, What are prions? proteinaceous infectious particle an infectious agent made only of protein, containing.

Biochemistry Part 3 Lehningers Biochemistry. Amino Acid.

Mad Cow Disease. Effects of Mad Cow disease Mad cow disease, or bovine spongiform encephalopathy (BSE), is a fatal brain disorder that occurs in cattle.

The Mammalian Digestive System

Prion Diseases Microbes and Society Fall What is a Prion? Prion- small proteinaceous infectious particles which resist inactivation by procedures.

Prion biology problem space: Mad cows, itchy sheep and protein structure.

Viruses Living or Not ???????.

 Laura Manuelidis argues a difference between a TSE AGENT and a PrP PATTERN.  Her research uses new techniques to attempt to define the differences.

Viruses, Viroids, and Prions

Presented by hadah al- khaldi

Transmissible Spongiform Encephalopathies (TSEs) a.k.a. Prion Diseases Transmissible  can be spread Spongiform  resembling a sponge Encephalopathies.

By Shon Augustine. Mad cow disease is an incurable, fatal brain disease that affects cattle and possibly some other animals, such as goats and sheep.

By : Amirah nu’aimi Sharifah Nurul Hanim TASK 2 – DISCUSS THE EXAMPLE OF PROTEIN FOLDING DISEASE BY STATING THE MECHANISM.

PRIONS PETER H. RUSSELL, BVSc, PhD, FRCPath, MRCVS Department of Pathology and Infectious Diseases, The Royal Veterinary College, Royal College Street,

1 An Introduction to the Viruses. 2 Viral Components All viruses have capsids- protein coats that enclose & protect their nucleic acid Viruses may have.

PRIONS Kalina Estrada TA: Yu-Chen Hwang Thursday, 7-8pm.

Prions and Protein Misfolding BICH 107 GENE 105. Kuru Discovered in Papua New Guinea in early 1900's "Trembling with fear" Characterized by headaches,

Diagnostics Alan S. Rudolph PhD MBA Chief Executive Officer Adlyfe Inc. Rockville, MD Innovation is in our blood Diagnostics.

Chronic Wasting Disease (CWD) David Litts. What is it? Prions (infectious incomplete protein) Prions (infectious incomplete protein) Creutzfeldt-jakob.

Removal of Infectious Prions from Red Cell Concentrates Samuel Coker, PhD Principal Scientist and Technical Director Pall Medical Transmissible Spongiform.

Digestion. Digestion – the process by which food is broken down into absorbable units.

Prion. Similar to Viruses Atypical Agents  Small  Filterable  Need host cells  No machinery for energy generation of protein synthesis.

John Gray, Sandor Dudas and Stefanie Czub

Prion cell tropism significantly varies among animal species, depending on both the agent strain and host-specific factors. For example, prions show high.

C B E R E R Vostal 10/2005 Labeling Claims for TSE Reduction Studies with Blood Processing Filters Jaro Vostal, M.D., Ph.D. Division of Hematology, OBRR.

TSE Agent Clearance Issues TSE Advisory Committee February 20, 2003 Dorothy Scott, M.D. DH/OBRR/CBER/FDA.

TSE Clearance in Plasma Derivatives TSE Advisory Committee February 8, 2005 Dorothy Scott, M.D. DH/OBRR/CBER/FDA.

Founders With the support of. 2 Letters of intent  Individual quotation, then selection by the SAB Validation by the Bureau  Full Application  Individual.

Prions_Stanley B. Prusiner Wen-Chun Shaw Wen-Chun Shaw BTC SCIENTIFIC DISCOVERY BTC SCIENTIFIC DISCOVERY Dr. Vankley Dr. Vankley.

The History of Chronic Wasting Disease Dr. Trent Bollinger, CCWHC One World, One Health Symposium Sept. 29, 2004.

MICROORGANISMS: Viruses, Prions, Archaea. What do Archaea and Bacteria have in common? Single celled Microscopic No membrane bound nucleus Both essential.

Removal of Infectious Prions from Red Cell Concentrates Blood Safety Advisory Committee March 17, 2005 Joseph Cervia, M.D., FACP,FAAP Professor of Clinical.

TSE Task Force Prion reduction evaluation in the manufacturing of plasma protein therapies Dr. Henry Baron, Chair, PPTA TSE Task Force.

Beyond Viruses…Beyond Viroids…

Prions “Scrapie” “mad cow disease” Nobel Prize 1997

Dr. Stuart Reichler Bio 311D. DateSubjectTextbook Chapters Jan 14Introduction, Philosophy of Science 16Strong Inferencearticle on webpage 18, 23Meiosis12,

Proteolytic Inactivation of prions; a biological solution to TSE decontamination. Dickinson J*, Murdoch H*, Sutton JM*, Crabb, W. D.#, Bott, R#, Penet,

Workshop on Bovine Spongiform Encephalopathy (BSE) control measures in EU member states Franco Mutinelli, Anna Granato Istituto Zooprofilattico Sperimentale.

Aya Murakami. Background Transmissible Spongiform Encephalopathies and Prion Other for TSEs CWD Distribution in the US Symptoms and Diagnosis Biomarkers.

+ High CJD infectivity remains after the prion protein is destroyed By Sylvester Gates.

(Bovine spongiform encephalopathy)

MICROBIAL FOOD SAFETY A FOOD SYSTEMS APPROACH

Rabies virus and Prion Dongli Pan

Digestive System Outline

KEY CONCEPT Infections can be caused in several ways.

In Vitro Generation of Infectious Scrapie Prions

Volume 113, Issue 1, Pages (April 2003)

Fig. 4. Prion infectivity of skin samples from sCJD patients.

Unit III The Digestive System and The Excretory System

Persistant viral infections of the central nervous system

Volume 27, Issue 2, Pages (August 2000)

Nat. Rev. Neurol. doi: /nrneurol

Grass Plants Bind, Retain, Uptake, and Transport Infectious Prions

Volume 27, Issue 2, Pages (August 2000)

Volume 134, Issue 5, Pages (September 2008)

Transfer of Scrapie Prion Infectivity by Cell Contact in Culture

Viruses Living or Not ???????.

DIGESTIVE SYSTEM ALIMENTARY CANAL DIAGRAM

Western blot analysis of skin tissue from CJD and non-CJD patients

What are Prions? A microscopic protein particle similar to a virus but lacking nucleic acid.

Presentation transcript:

Prion Digestion in the Alimentary Tract Nicholas Toney

Overview  Outline of the Digestive Tract  Dr. Manuelidis’s Claims  Experimental Procedure  Discussion

Ruminant Digestion

Digestion

Claim to Brain  Manuelidis’s Claim:  All detectable forms of PrP are digested in the gastro intestinal tract yet the invasive infectious particle (as many conventional viruses) is not destroyed

Transportation of prion protein across the intestinal mucosa of scrapie-susceptible and scrapie-resistant sheep  Experimental Methods and Goals  In Vivo GI inoculation  In Vitro GI digestion of infectious brain slurry

Western Blot

Infectivity of Scrapie Prion Protein (PrPSc) Following In vitro Digestion with Bovine Gastrointestinal Microbiota  Goals  Methodology  In Vitro Digestion  In Vivo determined level of infectivity

Discussion  Acid Resistance of Prions, digestion by microbiota  What limitations are there with the experimental methods?  Are the in-vitro digestion experiments a good model of digestion?  How do you reconcile the loss of detectable PrPres with the continued infectivity of brain slurry from Scrapie infected brain material?