Nikon Spinning Disk Imaging & Training Support. Find your cells with DIC in Live Preview 1.The green DIA Lamp, used for DIC, should be on. 2. Condenser.

Slides:

Advertisements

Similar presentations

CHDD BOL Equipment Primer Rooms CD 326 & CD 391 Behavioral Evaluation Center Behavior Observation Laboratory.

Advertisements

By Aaron Proia and Matthew Copenhaver.  For this presentation, we will be walking you through two processes that are commonly used in Photoshop.  These.

WELCOME Elmo and Video Projector Training Adapted from elmosprojectors/Elmo%20Training.ppt.

By: Kaitlyn Schroeder; ETEAMS 2014 Modified from Trimpe

Hand sectioning a mushroom 1)cut a wedge from cap that includes the gills2) Hold the section tightly in your fingers and try to shave off thin longitudinal.

Stem Cell Instrumentation Foundry (SCIF) Orientation: Microscopy Venu Polineni Research Associate, SCIF University of California, Merced Phone: (559)

Nikon Eclipse TE2000 Spinning Disc. inverted perfect focus microscope optimized for live cell imaging and time lapse movies Equipped for DIC, Ludl Piezo.

Mercury bulb Camera Microscope on/off Stage up/down Focus wheel Objective controls Fluorescence filter controls Mono/RGB.

 Any time you half press the shutter button, the light meter activates.  As we know, it measures the light in your scene, and calculates a shutter speed.

A. Imaging sample: 1. Place sample on stage 2. Make sure sample does not interfere with C mirrors 3. Start “Sonic MyDVD” on both laser heating computers.

Operation of the S4700 FESEM

Ms. Murphy.  Keep strongest light behind camera (at camera person’s back)  Reflectors redirect light  Silk or cloth can diffuse strong light.

Ms. Murphy.  Keep strongest light behind camera (at camera person’s back)  Reflectors redirect light  Silk or cloth can diffuse strong light.

Digital Photography: Nikon D-90 External Controls Lesson 1 Instructor: Ms. Davis.

A stereo dissecting microscope is normally used to view relatively large specimens at magnifications from about 5X to about 50X. The scope provides a very.

How a microscope works Microscopes create a magnified image using lenses. Light from a lamp is reflected by a mirror (1) up through the specimen on.

Parts of the Microscope and Their Function On the next slide there is an image of a microscope, very similar to the scopes we use in class. Click on the.

FNI 2B OM 1 Optical Microscopes. FNI 2B OM2 Outline Justification History Components of the Optical Microscope Theory of operation  Basic Microscope.

DIC Differential Interference Contrast Kim Peifley 08/14/15.

High Dynamic Range (HDR) Photography. Camera vs Eye Eye sees a wider range of color luminance than digital cameras HDR’s images compensate for this by.

Preparing a Camera for Shooting ENG EFP CONSUMER.

How to Use Digital Blue QX5 (See separate powerpoint on the use of the software)

Speech Room AV Capture Station Speech Room AV Capture Station Revision Date: November 2014.

Near IR Imaging on the UV510 Kim Peifley 07/22/14.

Remote Astronomy and Colour Imaging During these sessions you will learn how to make images from your own remote telescope observation just like this one.

Mercury bulb Camera Microscope on/off Stage up/down Focus wheel Objective controls Fluorescence filter controls Mono/RGB.

Microscope Control Configuration This section of the QED manual will describe how to configure you Configure and Control your automated microscope. Mac.

Photography Seeing through the camera’s eye. Vocabulary Definitions Photography: the art or technique of exposing light to an electronic sensor or film.

Taking Better Confocal Images Kim Peifley 2/09/15.

11/2/20151 THE MICROSCOPE Use of the Microscope Lab Manual Exercise # 2.

Making a Print How to use the Enlarger. The Enlarger.

» We have Nikons, so we will be looking at the Nikon mode dial » Most cameras use similar icons.

Microscope Basics.

Updated 710 Turn On/Off procedure Kim Peifley 12/18/14.

Setting up a trinocular dissecting microscope for best viewing

Introduction to the Microscope

Introduction to the Microscope  Care  Parts  Focusing.

Microscope Basics (1) Parts and focusing Label hand-out.

PHOTOGRAPHY FUNDAMENTALS Presented by Noah Hawthorne ALEXANDRA COPLEY.

The Microscope! What do we know about the microscope? Ex: Parts and usage of the microscope.

{ Aim: How can we process camera raw files?.  When you make adjustments to a camera raw image, Photoshop preserves the original camera raw file data.

Proper Exposure and Light Meter Basics Instructor: Ms. Davis.

ANATOMY OF A CAMERA Introduction to Photography. This is a camera.

T. Trimpe Body Tube B. Nosepiece Holds the High- and Low- power objective Lenses; can be rotated to change Magnification.

Microscopes…... Types….. 1 – Compound Light 2 – Transmission Electron (TEM) 3 – Scanning Electron (SEM)

The Digital Camera Canon Power Shot ELPH 100 HS.

Proper Handling Steps of the Microscope. Steps for Handling the Microscope 1.Carry the microscope by the arm and the support base if you can. 2.Ensure.

Care and use of Nikon 50i.

Compound light microscope. A Compound Light Microscope  Allows us to view specimens that are too small to be seen with the naked eye  The specimen must.

Introduction to the Microscope. Compound Microscope Dissection Microscope Scanning Electron Microscope (SEM)

Quality Enhancement Video Quality. Introduction ● This section will bring you through the following concepts: 1. How lighting and camera positioning enhance.

Microscopes Kinds Light –Image illuminated from bottom in most, top in a few. –Magnifies from times. –Can see individual cells and a few organelles.

Nature Abstracts Project 4 Marlee Odell.

TDA Direct Certification

How to Use a Microscope.

Setting up a trinocular compound microscope for best viewing

Gel Documentation system(Gel Doc)

Cameras and Lighting Ms. Murphy.

Microscope Tutorial.

Parts of the Microscope and Their Function

Proper Exposure and Light Meter Basics

Crew Position-Camera Operator

Lesson 1.4: Microscopes.

Its use in setting exposure

Parts of the Microscope and Their Function

Harvard Medical School Neurobiology Imaging Facility

How to use a compound microscope.

Harvard Medical School Neurobiology Imaging Facility

Michael Blanchard Imaging Technician

Presentation transcript:

Nikon Spinning Disk Imaging & Training Support

Find your cells with DIC in Live Preview 1.The green DIA Lamp, used for DIC, should be on. 2. Condenser Analyzer - must be “in” (i.e., green light is on) 3. Light Path – switch to “eye” 4. Filter – Switch it to the “open” position 5. Shutter – Flip 1 st switch up on shutter controller box. *Note: This one sticks sometimes. If no light, flip it off then on again.

Find your cells with DIC in Live Preview 5.Tell software that you are using the CoolSnap camera for Live Preview - Select “Camera” option - Select “Select Camera” - Click “OK” by camera 6. Find cells - Use knob on scope for coarse adjustment - Use box with dial for fine - Joystick moves the stage

1. Turn DIC off on shutter box 2. Turn on FL on shutter box (2 nd switch) 3.Select filter cube you want to use 4.Turn on PF 5. Turn off analyzer Find your cells with FL in Live Preview

5.Tell software that you are using the Cascade camera for Live Preview - Select “Camera” option - Select “Select Camera” - Click “OK” by camera 6.Select Live Preview 7.Click Ludl Filter Wheel 1 - Select the lasers you will use 8.Click AOTF - The AOTF must match the Ludl Filter Wheel selection. - Once you hit AOTF, cells are illuminated Find your cells with FL in Live Preview

Notes: -Live Preview window is small. Go to “Zoom In” and select it one time. *DO NOT MAXIMIZE THE WINDOW. -Leave on “Auto Enhance Contrast”. -Select Exposure time (i.e., 100 for dim or 600 ms for bright). -You can make adjustments to the histogram (i.e., white & black points). -DO NOT ADJUST GAMMA. It should be at Once the Perfect Focus is on, you must use the dial on the PF box to make final focus adjustments. -Stop the Live Preview when you are ready to run your script. Find your cells with FL in Live Preview