 Alcohols for fuel: Biological energy to usable energy Beth Papanek.

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Presentation transcript:

 Alcohols for fuel: Biological energy to usable energy Beth Papanek

Three main alcohols:  Methanol  Ethanol  Butanol

History of Ethanol biofuelsdigest.com

Potential Yield

History of Butanol  Produced commercially starting in the 1920’s Source: Hans Blaschek

Ethanol vs. Butanol Wikipedia: “Ethanol” “isobutanol”

Comparison Source: Hans Blaschek

Quick Biochem overview…

Saccharomyces cerevisiae

Butanol Metabolism Source: Hans Blaschek

Problem: Preferential Hexose consumption Source: bio.miami.edu 38-50% Cellulose, 15-30% Lignin, 23-32% Hemicellulose in cellulosic plants

Possible Improvements: Genetic Engineering  Saccharomyces cerevisiae  Gene expression for xylose uptake  Increased xylose transport  Clostridium beijerinckii  Inhibitor tolerance  Metabolic constraints  Electron flow

References Ezeji, T., Qureshi, N., Blaschek, H. (2007) Bioproduction of butanol from biomass: from genes to bioreactors. Current Opinion in Biotechnology. 18: Gray, K., Zhao, L., Emptage, M. (2006) Bioethanol. Current Opinion in Chemical Biology. 10: Nevoigt, E. (2008) Progress in Metabolic Engineering of Saccharomyces cerevisiae. Microbioloby and Molecular Biology Reviews. 72: