EAEC51 Study start 8-13-2013 N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control.

Slides:

Advertisements

Similar presentations

The FATZO mouse as a Translational Model for the Development of Drugs for Obesity, Metabolic Syndrome and Diabetes. PreClinOmics, Inc. 1.

Advertisements

Supplementary methods WST-1 assay of cell viability/proliferation. Raw264.7 or πBCL1 cells cultured with liposomes encapsulating dichloromethylene-bisphosphonate.

Figure S1. Zhao et al. a b c Figure. S1. VEGF does not stimulate population increase and cell cycle changes in breast cancer cells. (a) cells from.

Anti-CD40 and CpG induce activation of T cells in draining lymph nodes

Mice deficient in the NF-κB negative regulator, itch, develop severe osteoarthritis and reduced synovial lymphatic drainage due to m1 macrophages-induced.

Volume 16, Issue 2, Pages (July 2016)

Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. Partial knockdown of MPST markedly ameliorates hepatic steatosis in vivo. (A–C)

Fig. 4. NASH-driven fibrosis is partly TGF-β–dependent.

Volume 22, Issue 1, Pages (July 2015)

Mean and difference Raman spectra of spleen tissue from endotoxemic mice and sham mice and PCA-LDA. Mean and difference Raman spectra of spleen tissue.

Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Lung interleukin (IL)-33 protein expression in vivo depends on caspase-1. Mice.

Type 1 immunity drives metabolic disease but protects against NAFLD

Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient mice. Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient.

Fig. 5. Accelerated NASH-driven fibrogenesis in obese IFN-γ−/− mice is characterized by severe eosinophilic inflammation during TGF-β blockade. Accelerated.

Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. Effect of plumbagin (PL) on the growth of prostate tumors in Pten-KO mice. A.

Volume 23, Issue 4, Pages (April 2016)

Viperin−/− mice have high levels of pro-inflammatory cytokines but low levels of Th2 cytokines. Viperin−/− mice have high levels of pro-inflammatory cytokines.

Myung H. Kim, Elizabeth J. Taparowsky, Chang H. Kim Immunity

T cell–intrinsic STING inhibits cell cycle progression of T cells.

The relationship between the genes in mice and human samples

Dietary medium-chain triglycerides promote oral allergic sensitization and orally induced anaphylaxis to peanut protein in mice Jianing Li, MS, Yu Wang,

FGFR functions as a Pi sensor in vivo.

Responses to high Pi diet in Fgfr1-cKO mice.

Macrophage-resident NRP1 mitigates cytokine release and proinflammatory polarization. Macrophage-resident NRP1 mitigates cytokine release and proinflammatory.

IL-10R-deficient macrophages secrete IL-23, inducing IL-22 secretion by ILC3 and TH17 cells. IL-10R-deficient macrophages secrete IL-23, inducing IL-22.

Volume 20, Issue 4, Pages (October 2014)

Pulmonary cytokine expression in C57Bl/6 mice post IT-LPS.

Fig. 1. Body weight gain in Cbx7-KO mice

Fig. 1. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse model. IL-31 and IL-31R mRNA expression was upregulated in the asthma mouse.

Cellularity of epididymal adipose tissue.

IRF4 expression is modulated by the signaling of IL-2 family cytokines

GSIS. GSIS. In vivo: serum insulin levels at fasting state and 30 min after glucose injection (A) and the fold change in serum insulin after glucose loading.

Expression and Activity of Methylation-Associated Proteins In Vivo and In Vitro (AandB) The protein level of methylation-associated proteins (DNMT1, DNMT3a,

TSG-6 suppressed APC activation in vitro and in vivo.

FGFR1 and βKlotho are expressed in the hypothalamus of mice.

IRF4 expression regulates the sensitivity of CD8+ T cells to IL-2, IL-15, and IL-7. IRF4 expression regulates the sensitivity of CD8+ T cells to IL-2,

GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. GLUT4 LXRE is required for downregulation of adipose.

Skeletal muscle characteristics of HFD-induced obese mice.

Expression of cGMP signaling components in polyps.

CD8α+, CD8α−, and MoDCs from NOD.hCD205 mice express hCD205.

Effect of berberine on white adipose tissue mass.

Reduced β-cell mass in MCH-KO mice on normal chow and high-fat diets.

Metabolic parameters of Id1−/− and wild-type mice fed a standard chow diet (hatched bars and striped bars, respectively) or a high-fat diet (black bars.

MϕRIP140KD mice show browning in vWAT.

Fig. 7. Nrf2-dependent enzyme activities in wild-type, Nrf2- and Keap1-deficient tissues.Hepatic (A,C,E) and cortical (B,D,F) enzyme activities of NQO1.

Members of IL-1 family of cytokines favor the generation of IL-3–secreting CD4+ T cells in vitro. Members of IL-1 family of cytokines favor the generation.

Hepatic fuel metabolism in male 5αR1-KO and WT mice

Hepatic PPARγ deficiency impairs the SCFA-induced reduction in hepatic steatosis. Hepatic PPARγ deficiency impairs the SCFA-induced reduction in hepatic.

Treatment of high-fat diet–fed mice with TTR-ASOs decreases circulating TTR and RBP4 levels, and improves insulin sensitivity. Treatment of high-fat diet–fed.

MϕRIP140KD mice exhibit improved metabolic phenotypes.

MMP expression at the growth plate.

High-fat diet–induced glucose intolerance is prevented in ghrelin knockout (Ghr-KO) mice. High-fat diet–induced glucose intolerance is prevented in ghrelin.

β-Cell–specific deletion of Phb2 renders mice diabetic.

Mice lacking Y1 receptor in the hematopoietic compartment remain healthy under normal chow feeding conditions. Mice lacking Y1 receptor in the hematopoietic.

Regional and cellular differences in Kcnq4 transcripts in the cochlea.

Increased anti-DNA Abs in 2KO-Bcl6TC mice.

Deficiency of adipocyte IKKβ does not affect diet-induced weigh gain but results in an exaggerated diabetic phenotype when challenged with an HFD. A: IKKβ.

TFAP2A knockdown inhibits tumor growth in vivo.

Figure 1. T-cell immunogenicity of HZ DNA vaccines in C57BL/6 mice

Immunization regimens that include a GLA-SE-formulated protein vaccine generate memory CD4 T cells. Immunization regimens that include a GLA-SE-formulated.

Rapamycin decreases PD-L1 expression in lung tumors in vivo.

Endothelial cells/microvasucles are increased in prostate cancer versus normal tissues. Endothelial cells/microvasucles are increased in prostate cancer.

Effect of inhibiting HO-1 on adaptive immune- and cytokine-dependent regulation of tumor growth. Effect of inhibiting HO-1 on adaptive immune- and cytokine-dependent.

IDO deficiency is associated with attenuated induction of IL-6 during 4T1 tumor metastasis. IDO deficiency is associated with attenuated induction of IL-6.

Fig. 7 BEL immune response.

AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. A,

In vivo inhibition of IL-17 abrogates tumor-promoting effect of myeloid cells. In vivo inhibition of IL-17 abrogates tumor-promoting effect of myeloid.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

PD-L1 expression is not associated with PTEN expression status in melanomas. PD-L1 expression is not associated with PTEN expression status in melanomas.

Spontaneous PD in DRB1*04:01 mice.

Presentation transcript:

EAEC51 Study start N or NZD fed 14d/ab pretreatment/10^9 EAEC 042 EAEC51 Study Design Objectives/Questions Repeat EAEC40 with nourished control diet made with egg white protein Generate additional data for Bolick, et al manuscript Day mice delivered -3 Tues -14 Tues Design 1)C57Bl/6 male mice 4 weeks old on arrival 2)Nourished (N) or Nourished-Zinc (NZD) diet fed for two weeks 3) Antibiotic cocktail pretreatment for 2 days, followed by 1 day off 4) weights, stools collected every day, sac day 3 EAEC weights and stools Diet Start Thurs ab Euthanasia for collection 3 Zinc depletion

To do for EAEC 51 Mx Histology (done, needs pics and IHC for CD45) qPCR for cytokines qPCR for in vivo virulence factor expression Final revisions and where to submit

IL-17r Study

IL-17rKO To Do Histology (done, needs pics and IHC?) qPCR (Th17 pathway plate) Tissue burden (done, needs graph) Mice are in, MTA is still in approval process IL-25?