Carly Sproule BE 4332.  Mechanism that silences a gene so that the protein associated with it is not expressed  Regulates gene expression  Used as.

Slides:

Advertisements

Similar presentations

Dogmatic View of Gene Expression DNAProteinRNA Post-transcriptional Control: Quantitative Control: Levels of mRNA not proportional to levels of mRNA synthesized.

Advertisements

Rhiana Lau MMG C174 Professor Simpson

Identification of the Plant Systemic RNA Silencing Signal 2008 Summer HHMI Program Simon Johnson Mentors: Dr. James C. Carrington – Professor and Director.

Gene 2 Nerve cells Neuron-specific enhancer P2 T Ex2Ex3 Ex4I3I2 Cell-specific gene expression gene 1 P1 T Ex1Ex2 Ex3I2I1 Ex1 I1 Skin cell-specific enhancer.

Dicer-Like A RNA Helicase Gene Involved in Gene Silencing UCSF Max BachourJessica ChenChris McQuilkin.

1 Review What genes control cell differentiation during development Compare and Contrast How is the way Hox genes are expressed in mice similar and different.

Lesson Overview Lesson Overview Gene Regulation and Expression Lesson Overview 13.4 Gene Regulation and Expression.

Advantages of C. elegans: 1. rapid life cycle 2. hermaphrodite

Can RNA form base pairs? Yes, in many circumstances Some are strictly dsRNA molecules, others are folded back pieces of ssRNA with interactions between.

Transgenic Organisms.

RNAi. What is RNAi? RNA-based mechanisms of gene silencing. These siRNAs are bound by a protein-RNA complex called the RNA-induced silencing complex (RISC)

Gene Technology Section 1: Genetic Engineering

Genetic Frontiers Chapter 15.

Transfection. What is transfection? Broadly defined, transfection is the process of artificially introducing nucleic acids (DNA or RNA) into cells, utilizing.

BEH. 109: Laboratory Fundamentals in Biological Engineering

Hypothetical Situation

RNA interference Definition: RNA interference (RNAi) is a mechanism where the presence of certain fragments.

MBP1007/ Nucleic Acids A functional mRNA: The cytoplasmic story Objectives (1) To discuss the iNUTS and iBOLTS of how mRNAs function in the cytoplasm.

SiRNA and Epigenetic Asma Siddique Saloom Aslam Syeda Zainab Ali.

Changes to Syllabus: Quizzes put back: Change Oct. 3 to Oct. 17

What is RNA interference?

Welcome Everyone. Self introduction Sun, Luguo ( 孙陆果） Contact me by Professor in School of Life Sciences & National Engineering.

RNnomics Advanced MCB Presentation Series I Group Members: 孙群, 刘军, 段文霞，刘少英.

Arabidopsis thaliana Response to Tobacco Rattle Virus Jessica Martin, Cory Zoetewey, and Lisa K. Johansen, Department of Biology University of Colorado.

Conditional systems - principles Conditional systems may function on the basis of: - regulatory proteins - aptamers - allosteric ribozymes - antisense.

Characterization of RDR Gene Expression Johnny R. Nunez and Lisa K. Johansen Community College of Denver and University of Colorado at Denver and Health.

CHP: 13 BIOTECHNOLOGY. GENETIC ENGINEERING  The procedure for cleaving DNA from an organism into smaller fragments & inserting the fragments into another.

Gene Regulation and Expression. Learning Objectives  Describe gene regulation in prokaryotes.  Explain how most eukaryotic genes are regulated.  Relate.

The Power of “Genetics” LOSS OF FUNCTION Easy in yeast Difficult in mammals Powerful tool to address roles in developmental or signaling networks Gene.

Directed Reading: “Biology in Your World”

Researchers use genetic engineering to manipulate DNA. Section 2: DNA Technology K What I Know W What I Want to Find Out L What I Learned.

Advantages of C. elegans: 1. rapid life cycle 2. hermaphrodite 3. prolific reproduction 4. transparent 5. only ~1000 cells 6. laser ablation 7. complete.

Small interfering RNA. Petunias White pigmentPurple pigment Chalcone synthase.

Control of Gene Expression. Ways to study protein function by manipulating gene expression Mutations –Naturally occurring, including human and animal.

RNA-ligand interactions and control of gene expression

Viral Silencing Suppressors. Tools forged to fine-tune host-pathogen coexistence.

Vectors for RNAi.

By: Jack Liao- HOSTOS- Lincoln Academy of Science Jingwei Zhang- H.S. for Dual Language and Asian Studies Gabriell Copeland- Eagle Academy For Young Men.

Atta ur Rahman school of biological Science National University of Sciences & Technology CMB-234:Molecular Biology.

Ch 16. Posttranscriptional Regulation RNA interference (RNAi)

Lecture 8 Ch.7 (II) Eukaryotic Gene Regulation. Control of Gene Expression in Eukaryotes: an overview.

Non-Coding RNA Helen Nordquist November 13, 2015.

Interfering RNA (干擾RNA)

Conditional systems - principles

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

Figure 2 Dicer and RISC (RNA-induced silencing complex).

Biogenesis of micro (mi) and silencing (si)RNAs

I Virus e l’RNAi approfondimenti.

A-LEVEL BIOLOGY RNA interference (RNAi)

Genetic Engineering 9/11/2018 SB2f.

Gene Expression Dr. Kevin Ahern.

Chapter 18 Gene Expression.

Genetic Engineering and Gene Expression

Steps in microRNA gene silencing

Short interfering RNA siRNA

Cell Signaling.

Short interfering RNA siRNA

Short interfering RNA siRNA

New Hope for a MicroRNA Therapy for Liver Cancer

In the name of god, the compassionate, the merciful Antisense RNA Dr.sharif Moghaddam By: Niloofar Beiranvand.

RNA interference: the new somatic cell genetics?

Unit III Information Essential to Life Processes

Antiviral RNA-induced silencing complexes

RNAi: Prokaryotes Get in on the Act

siRNA / microRNA epigenetics stem cells

Molecular Therapy - Nucleic Acids

The Role of RNA Editing by ADARs in RNAi

Engineering parathyroid cells to treat secondary hyperparathyroidism

The Power of “Genetics”

Presentation transcript:

Carly Sproule BE 4332

 Mechanism that silences a gene so that the protein associated with it is not expressed  Regulates gene expression  Used as an immune response and natural defense mechanism  Can be used for certain gene therapies in humans as well as having agricultural uses

 A piece of double stranded RNA(dsRNA) triggers the process  RDE-4 and RDE-1 must be present to signal the Dicer molecule (DCR-1)  Dicer cuts a short- interferening RNA (siRNA)  This siRNA has a two nucleotide overhang on the 3’ ends(sticky ends)

 The guide strand of the siRNA complexes to form the RNA Induced Silencing Complex (RISC)  Slicer is part of the activated RISC complex  The guide strand finds a complementary sequence so Slicer can chop that piece of RNA thereby silencing the gene

 In plants, fungi, and some animals, RNA dependent RNA polymerases(RDRP) amplify the RNAi mechanism  m/watch?v=gZZyxVP02U U m/watch?v=gZZyxVP02U U

 Cancer  Auto-immune diseases  Viral infections  Dominant genetic disorders

 Virus resistant plants and crops  Plants that produce dsRNA that silence specific and essential genes in pests  Create sterile hybrids  Enhance nutrient content

 New techniques to combat diseases ◦ Cure for cancer?  Pesticides may become a thing of the past ◦ Increased crop yield ◦ Safer for consumption  Fewer malnourished peoples