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Slide 1 of 24 Copyright Pearson Prentice Hall Biology

Slide 2 of 24 Copyright Pearson Prentice Hall 14–3 Human Molecular Genetics 14-3 Human Molecular Genetics

14–3 Human Molecular Genetics Slide 3 of 24 Copyright Pearson Prentice Hall Human DNA Analysis There are roughly 6 billion base pairs in your DNA. Biologists search the human genome using sequences of DNA bases.

14–3 Human Molecular Genetics Slide 4 of 24 Copyright Pearson Prentice Hall Human DNA Analysis Genetic tests are available for hundreds of disorders. DNA testing can pinpoint the exact genetic basis of a disorder.

14–3 Human Molecular Genetics Slide 5 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA Fingerprinting DNA fingerprinting analyzes sections of DNA that have little or no known function but vary widely from one individual to another. Only identical twins are genetically identical. DNA samples can be obtained from blood, sperm, and hair strands with tissue at the base.

Slide 6 of 24 14–3 Human Molecular Genetics Copyright Pearson Prentice Hall Human DNA Analysis Chromosomes contain large amounts of DNA called repeats that do not code for proteins. This DNA pattern varies from person to person.

14–3 Human Molecular Genetics Slide 7 of 24 Copyright Pearson Prentice Hall Human DNA Analysis Restriction enzymes are used to cut the DNA into fragments containing genes and repeats.

14–3 Human Molecular Genetics Slide 8 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA fragments are separated using gel electrophoresis. Fragments containing repeats are labeled. This produces a series of bands—the DNA fingerprint.

14–3 Human Molecular Genetics Slide 9 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA Fingerprint

14–3 Human Molecular Genetics Slide 10 of 24 Copyright Pearson Prentice Hall The Human Genome Project What is the goal of the Human Genome Project?

14–3 Human Molecular Genetics Slide 11 of 24 Copyright Pearson Prentice Hall The Human Genome Project In 1990, scientists in the United States and other countries began the Human Genome Project. The Human Genome Project is an ongoing effort to analyze the human DNA sequence. In June 2000, a working copy of the human genome was essentially complete.

14–3 Human Molecular Genetics Slide 12 of 24 Copyright Pearson Prentice Hall The Human Genome Project Research groups are analyzing the DNA sequence, looking for genes that may provide clues to the basic properties of life. Biotechnology companies are looking for information that may help develop new drugs and treatments for diseases.

14–3 Human Molecular Genetics Slide 13 of 24 Copyright Pearson Prentice Hall The Human Genome Project A Breakthrough for Everyone Data from publicly supported research on the human genome have been posted on the Internet on a daily basis. You can read and analyze the latest genome data.

14–3 Human Molecular Genetics Slide 14 of 24 Copyright Pearson Prentice Hall Gene Therapy What is gene therapy?

14–3 Human Molecular Genetics Slide 15 of 24 Copyright Pearson Prentice Hall Gene Therapy In gene therapy, an absent or faulty gene is replaced by a normal, working gene. The body can then make the correct protein or enzyme, eliminating the cause of the disorder.

14–3 Human Molecular Genetics Slide 16 of 24 Copyright Pearson Prentice Hall Gene Therapy Viruses are often used because of their ability to enter a cell’s DNA. Virus particles are modified so that they cannot cause disease. Normal hemoglobin gene Genetically engineered virus

14–3 Human Molecular Genetics Slide 17 of 24 Copyright Pearson Prentice Hall Gene Therapy A DNA fragment containing a replacement gene is spliced to viral DNA. Chromosomes Bone marrow cell Nucleus Genetically engineered virus

14–3 Human Molecular Genetics Slide 18 of 24 Copyright Pearson Prentice Hall Gene Therapy The patient is then infected with the modified virus particles, which should carry the gene into cells to correct genetic defects.

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