1. End Show Slide 1 of 24 Copyright Pearson Prentice Hall Biology

2. End Show Slide 2 of 24 Copyright Pearson Prentice Hall 14–3 Human Molecular Genetics 14-3 Human Molecular Genetics

3. End Show 14–3 Human Molecular Genetics Slide 3 of 24 Copyright Pearson Prentice Hall Bioethics and You As you become more aware of scientific advances in genetics, you might realize that with the ability to manipulate genes, there comes responsibility. This ability provides an opportunity to improve the lives of many people. But there is also a potential for errors or intentional misuse of the technology. Section 14-3 Interest Grabber

4. End Show 14–3 Human Molecular Genetics Slide 4 of 24 Copyright Pearson Prentice Hall Working with a partner, answer the following questions. 1.In what type of situation do you think genetic engineering— changing the genes of organisms—is warranted? Explain your reasoning about your position. If you do not think that genetic engineering is ever warranted, explain your reasons for your position. 2.In what type of situation do you think genetic engineering might be misused? Suggest limits that might be placed on the manipulation of genes to avoid its misuse. Section 14-3 Interest Grabber continued

5. End Show 14–3 Human Molecular Genetics Slide 5 of 24 Copyright Pearson Prentice Hall Human DNA Analysis There are roughly 6 billion base pairs in your DNA. Biologists search the human genome using sequences of DNA bases.

6. End Show 14–3 Human Molecular Genetics Slide 6 of 24 Copyright Pearson Prentice Hall Human DNA Analysis Genetic tests are available for hundreds of disorders. DNA testing can pinpoint the exact genetic basis of a disorder.

7. End Show 14–3 Human Molecular Genetics Slide 7 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA Fingerprinting DNA fingerprinting analyzes sections of DNA that have little or no known function but vary widely from one individual to another. Only identical twins are genetically identical. DNA samples can be obtained from blood, sperm, and hair strands with tissue at the base.

8. End Show Slide 8 of 24 14–3 Human Molecular Genetics Copyright Pearson Prentice Hall Human DNA Analysis Chromosomes contain large amounts of DNA called repeats that do not code for proteins. This DNA pattern varies from person to person.

9. End Show 14–3 Human Molecular Genetics Slide 9 of 24 Copyright Pearson Prentice Hall Human DNA Analysis Restriction enzymes are used to cut the DNA into fragments containing genes and repeats.

10. End Show 14–3 Human Molecular Genetics Slide 10 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA fragments are separated using gel electrophoresis. Fragments containing repeats are labeled. This produces a series of bands—the DNA fingerprint.

11. End Show 14–3 Human Molecular Genetics Slide 11 of 24 Copyright Pearson Prentice Hall Human DNA Analysis DNA Fingerprint

12. End Show 14–3 Human Molecular Genetics Slide 12 of 24 Copyright Pearson Prentice Hall The Human Genome Project What is the goal of the Human Genome Project?

13. End Show 14–3 Human Molecular Genetics Slide 13 of 24 Copyright Pearson Prentice Hall The Human Genome Project In 1990, scientists in the United States and other countries began the Human Genome Project. The Human Genome Project is an ongoing effort to analyze the human DNA sequence. In June 2000, a working copy of the human genome was essentially complete.

14. End Show 14–3 Human Molecular Genetics Slide 14 of 24 Copyright Pearson Prentice Hall The Human Genome Project Research groups are analyzing the DNA sequence, looking for genes that may provide clues to the basic properties of life. Biotechnology companies are looking for information that may help develop new drugs and treatments for diseases.

15. End Show 14–3 Human Molecular Genetics Slide 15 of 24 Copyright Pearson Prentice Hall PromoterStart signal Gene Stop signal

16. End Show 14–3 Human Molecular Genetics Slide 16 of 24 Copyright Pearson Prentice Hall The Human Genome Project A Breakthrough for Everyone Data from publicly supported research on the human genome have been posted on the Internet on a daily basis. You can read and analyze the latest genome data.

17. End Show 14–3 Human Molecular Genetics Slide 17 of 24 Copyright Pearson Prentice Hall Gene Therapy What is gene therapy?

18. End Show 14–3 Human Molecular Genetics Slide 18 of 24 Copyright Pearson Prentice Hall Gene Therapy In gene therapy, an absent or faulty gene is replaced by a normal, working gene. The body can then make the correct protein or enzyme, eliminating the cause of the disorder.

19. End Show 14–3 Human Molecular Genetics Slide 19 of 24 Copyright Pearson Prentice Hall Gene Therapy Viruses are often used because of their ability to enter a cell’s DNA. Virus particles are modified so that they cannot cause disease. Normal hemoglobin gene Genetically engineered virus

20. End Show 14–3 Human Molecular Genetics Slide 20 of 24 Copyright Pearson Prentice Hall Gene Therapy A DNA fragment containing a replacement gene is spliced to viral DNA. Chromosomes Bone marrow cell Nucleus Genetically engineered virus

21. End Show 14–3 Human Molecular Genetics Slide 21 of 24 Copyright Pearson Prentice Hall Gene Therapy The patient is then infected with the modified virus particles, which should carry the gene into cells to correct genetic defects.

22. End Show - or - Continue to: Click to Launch: Slide 22 of 24 Copyright Pearson Prentice Hall 14–3

23. End Show Slide 23 of 24 Copyright Pearson Prentice Hall 14–3 DNA fingerprinting analyzes sections of DNA that have a.Little or no known function but are identical from one individual to another. b.little or no known function but vary widely from one individual to another. c.a function and are identical from one individual to another. d.a function and are highly variable from one individual to another.

24. End Show Slide 24 of 24 Copyright Pearson Prentice Hall 14–3 DNA fingerprinting uses the technique of a.gene therapy. b.allele analysis. c.gel electrophoresis. d.gene recombination.

25. End Show Slide 25 of 24 Copyright Pearson Prentice Hall 14–3 Repeats are areas of DNA that a.do not code for proteins. b.code for proteins. c.are identical from person to person. d.cause genetic disorders.

26. End Show Slide 26 of 24 Copyright Pearson Prentice Hall 14–3 Data from the human genome project is available a.only to those who have sequenced the DNA. b.to scientists who are able to understand the data. c.by permission to anyone who wishes to do research. d.to anyone with Internet access.

27. End Show Slide 27 of 24 Copyright Pearson Prentice Hall 14–3 Which statement most accurately describes gene therapy? a.It repairs the defective gene in all cells of the body. b.It destroys the defective gene in cells where it exists. c.It replaces absent or defective genes with a normal gene. d.It promotes DNA repair through the use of enzymes.

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