Japan Environment and Children’s Study (JECS) I4C Barcelona, Spain 2011 Update: Progress to date Cohort summary  Years of recruitment: January, 2011 –

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Japan Environment and Children’s Study (JECS) I4C Barcelona, Spain 2011 Update: Progress to date Cohort summary  Years of recruitment: January, 2011 – December, 2013 (3 years)  Number of live births at baseline: 100,000 children  Follow up status: Follow-up programs will be implemented until the participant children reach at the age of 13.  Biospecimen Inventory Biospecimens Environmental samples Acknowledgements  The recruitment of pregnant women was started in January, Pregnant women should be T1, 12 ~16 weeks of gestation.  Japan Environment and Children’s Study International Symposium was held in Tokyo on February 4,  An open working group meeting on birth cohort studies is scheduled to be held at the International Society for Environmental Epidemiology (ISEE) conference in Barcelona, Spain on September 17, Eisaku TODA, Kei MORI Ministry of the Environment, Japan Hiroshi SATOH National Institute for Environmental Studies, Japan SAMPLE COLLECTED ( YES/NO) TIMING OF SAMPLE COLLECTION (weeks gestation / months of age) LOCATION OF SAMPLE COLLECTION (e.g. child’s bedroom) CONDITIONS OF STORAGEMONTHS SINCE STORED TOTAL NUMBER OF SAMPLES COLLECTED NUMBER OF SAMPLES AVAILABLE SEPT 2011 PrenatalFirst year of life Indoor air sampleTBD Dwelling dust collectionTBD Soil sampleTBD Drinking waterTBD Hair sample from petsTBD Others:TBD Geographic Information Systems (GIS): Utilize participants’ home addresses obtained from questionnaire. Others: 1. Daily monitoring data of ambient air quality 2. Results of measurement of surface water quality  Population sample Update: Primary outcomes  Planning to use a national cancer registration.  SAMPLE TIMING ( Indicate YES (Y) or NO (N) AND weeks gestation/months of age) CONDITIONS OF STORAGEMONTHS SINCE STORED BRIEF DESCRIPTION OF COLLECTION METHOD TOTAL NUMBER OF SAMPLES COLLECTED NUMBER OF SAMPLES AVAILABLE SEPT 2011 Prenatal (Mother) At birth (Mother/Child) First year of life (Child) Blood Y / T1*, T2*Y /Mother N (Collected at 6 years old) Stored at -80 ℃. For long-term storage, stored at -150 ℃. T1*: collect 32 ml. T2*: collect 33 ml. At birth: collect 18 ml.100,000 (plan) Serum Y/ T1, T2Y/MotherN Stored at -80 ℃. For long-term storage, stored at -150 ℃. T1: collect 32 ml. T2: collect 33 ml. At birth: collect 18 ml.100,000 (plan) Urine Y/ T1, T2N N (Collected at 6 years old) Stored at -80 ℃. For long-term storage, stored at -150 ℃. T1: collect 35 ml. T2: collect 25 ml.100,000 (plan) Saliva NNN Buccal swab NNN Vaginal swab NNN Cord blood NY/ChildNInsert a needle into an umbilical vein and collect cord blood 35 ml.100,000 (plan) Blood spots NY/ChildNDNA is stored at normal temperature. TSH is stored at -80 ℃. Collect a few drops of blood from a baby in a few days after birth.100,000 (plan) Placenta NNN Meconium NNN Hair sample NY/Mother & ChildNStored at normal temperature.Take 3 cm from the root of hairs.100,000 (plan) Nail sample NNN Breast milk NY/MotherN Stored at -80 ℃. Collect breast milk in one month after delivery.100,000 (plan) Milk teeth NNN OthersNNN Explanatory note: T1* means 12~16 weeks of gestation. T2* means 22~28 weeks of gestation.