(a) Structure of Mass Spectrometer

Slides:

Advertisements

Similar presentations

MASS SPECTROMETRY AP Chemistry Big Idea 1

Advertisements

D e t e c t o r s f o r H P L C.

J. J. Thompson was able to separate two neon isotopes (Ne-20 and Ne-22) in 1913, which was the first evidence that isotopes exist for stable elements.

Chapter 20 Molecular Mass Spectrometry Mass spectrometry is capable of providing information about (1) the elemental composition of samples of matter.

Andrew Rouff and Ben Berger

What is this ROCK??? Any guesses? Ok clue!!! It is not from Earth Yesss!! MARS!! Is there any way, we can figure out what its compounds are? What is it.

Advanced Higher Unit 3 Mass Spectrometry. Mass spectrometry can be used to determine the accurate molecular mass and structural features of an organic.

Module 2: Analytical methods and separation techniques.

Mass spectroscopy. In a typical MS procedure:  1- a sample is loaded onto the MS instrument, and undergoes vaporization.  2- the components of the sample.

The Mass Spectrometer Topic 2.2. Review of Topic 2.1.

Mass Spectroscopy Skyline IB Chemistry HL Mass Spectroscopy.

12-1 Molecular Mass Spectroscopy Molecular structure Composition of mixtures Molecular mass spectra Ion Source Mass Spectrometers Applications.

Lecture 9. MALDI/TOF. Introduction Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry, allowing.

Atomic Mass is not a whole number

Instrumental Chemistry Chapter 11 Atomic Mass Spectrometry.

Atomic Mass Spectrometry

Mass Spectroscopy 1Dr. Nikhat Siddiqi. Mass spectrometry is a powerful analytical technique that is used to identify unknown compounds, to quantify known.

Mass Spectrometer Mass spectrometere is a very powerful method to analyse the structure of organic compounds.

4. Mass Spectrometry Objectives:

AUSTEN K. SCRUGGS CALIFORNIA STATE UNIVERSITY, FRESNO RIMI FACILITY INSTRUMENTATION.

Analytical Chemistry Option A Part 1: Mass Spectrometry & H-NMR.

Atomic Structure 2.2: The Mass Spectrometry. Operation of Mass Spec Describe and explain the operation of a mass spectrometer What’s it for? A mass spectrometer.

1 Chemical Analysis by Mass Spectrometry. 2 All chemical substances are combinations of atoms. Atoms of different elements have different masses (H =

Introduction to mass spectrometry. Electron Impact Ionisation Mass Spectrometry (EIMS) The sample is vaporised and ionised in the ion source of the.

William Kou Jonathan Mertz. Introduction The field of Mass Spectrometry using Ambient Ionization techniques has grown exponentially since The direct.

Mass Spectroscopy 1 Mass Spectroscopy (Mass Spec) Applying Atomic Structure Knowledge to Chemical Analysis.

Mass Spectroscopy Mass spectroscopy is a powerful tool in organic chemistry that can be used to provide information about the molecular formula and structural.

MASS SPECTROMETRY. CONTENTS Prior knowledge Background information The basic parts of a mass spectrometer The four stages of obtaining a spectrum How.

Chemistry Topic: Atomic theory Subtopic : Mass Spectrometer.

Ionization energy?. Ionization energy? EI Ionization??

Combining and Choosing Analytical Techniques Chapter 8.

Starter: Spec links 1.06–1.10. Green pens out! Spec links 1.06–1.10.

LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

Separates charged atoms or molecules according to their mass-to-charge ratio Mass Spectrometry Frequently.

Lecture 4b Mass Spectrometry.

CONTENTS Prior knowledge Background information The basic parts of a mass spectrometer The four stages of obtaining a spectrum How different ions are deflected.

SCC Science Dept F W Aston SCC Science Dept Aston’s Mass Spectrometer.

THE MASS SPECTROMETER describes how a mass spectrum is produced using a mass spectrometer.

Mass Spectrometry Relative atomic masses and the mass of individual isotopes can be determined using a mass spectrometer. The principle behind mass spectrometry.

Mixtures of Isotopes In nature, elements occur as a mixture of isotopes.In nature, elements occur as a mixture of isotopes. Average atomic mass = weighted.

Mass Spectrometry(MS). What does the mass spectrometer tell us? Very accurate determination of molecular mass of small compounds (or more accurately the.

Prepared by Lawrence Kok Tutorial on Mass Spectrometer and Isotopes.

CHROMATOGRAPHY Chromatography is used to separate and analyse small amounts of mixtures Methods involve a stationary phase and a mobile phase. There are.

What is Mass Spectrometry? Mass spectrometry could be considered as an analytical technique that involves the study in the gas phase of ionized molecules.

Mass Spectroscopy “You can’t make an omelette without breaking eggs” The Joker.

2008 SPECIFICATIONS HPLC and GAS CHROMATOGRAPHY A guide for A level students KNOCKHARDY PUBLISHING.

Atomic structure L.O. To be able to recall definitions of relative atomic, isotopic and molecular mass. To demonstrate an understanding of the basic.

The Mass Spectrometer Honours

A guide for A level students KNOCKHARDY PUBLISHING

Atomic structure L.O. 1. To be able to recall definitions of relative atomic, isotopic and molecular mass. 2. To learn about the key principles of mass.

Starter Complete the quiz and hand in..

Unit Objectives 1)understand the evolution of models of the atom: Dalton, Thomson, Rutherford, Bohr, the ‘quantum model 2) understand the meaning of mass.

Chapter 11 – Analytical Chemistry section

A guide for A level students KNOCKHARDY PUBLISHING

Instrumental Chemistry

Time-of-Flight Mass Analyzers

Mass Spectrometry.

Lecture 22 Introduction to Mass Spectrometry Lecture Problem 7 Due

A sample is injected into the spectrometer and heated to a vapour.

Mass spectrometry Detection of ionic species to determine the molecular weight of and obtain structural information on a molecule.

The Mass Spectrometer.

Mass Spectroscopy Mass spectroscopy is a powerful tool in organic chemistry that can be used to provide information about the molecular formula and structural.

Mass Spectrometry THE MAIN USE OF MS IN ORG CHEM IS:

M.Prasad Naidu MSc Medical Biochemistry, Ph.D,.

A guide for A level students KNOCKHARDY PUBLISHING

CHROMATOGRAPHY.

Mass spectrometry (MS) is an analytical technique that can be used to determine the mass, elemental composition or chemical structure of molecules. Mass.

Presentation transcript:

(a) Structure of Mass Spectrometer A=Vaporization Chamber B=Ionization Chamber C=Accelerating Chamber D=Deflection Chamber E=Ions detector

(a) Functions for each parts of Mass Spectrometer A=Vaporization Chamber B=Ionization Chamber C=Accelerating Chamber D=Deflection Chamber E=Ions detector to vaporize the sample to be tested by heat molecules are bombarded by alpha/beta rays to form cations cations are accelerated by a strong electric field cations are deflected by strong magnetic field according to their mass/charge ratio amounts of ions are proportional to the electric current detected

(b) Give two reasons why such kind of mass spectrometer cannot be used to determine the molecular mass of biomolecules. (1) Because biomolecules are thermal labile, they would decompose in the evaporation chamber under strong heating (2) The biomolecules would be cracked by the bombardment with alpha/beta rays in the ionisation chamber

(b) Name the two parts in the traditional mass spectrometer which were replaced by electrospray ionization (ESI) technique. Explain why such a technique can be used to study biological macromolecules. ESI technique can replace the vaporization chamber and ionization chamber. As no heating and bombardment with high energy particles to the bio-macromolecules are required by using electrospray ionization technique, the bio-molecules would not decompose.

(d) Name the two parts in the traditional mass spectrometer which were replaced by soft laser desorption (SLD) technique. Explain why such a technique can be used to study biological macromolecules. SLD technique can replace the vaporization chamber and ionization chamber. As no heating and bombardment with high energy particles to the bio-macromolecules are required by using soft laser desorption technique, the bio-molecules would not decompose.

(e) State two advantages of applying ESI and SLD techniques in Mass Spectrometer to study biological macromolecules. The method is superior to other methods in the rapidity, sensitivity and identification of the actual interaction. This analytical methods are relatively cheap, enabling them to spread quickly to laboratories all around the world. By having a surface that cancer cells adhere to – and then analysing this with soft laser desorption – chemists can discover cancer faster than doctors can.