Fecal calprotectin DR Amin Eftekhari

introduction Lower abdominal complaints are common in primary care Organic bowel disease (OBD) is rare: Colorectal cancer, advanced adenomatous polyps Inflammatory bowel disease (IBD) Symptoms of OBD overlap with benign and functional bowel disorders Diagnostic challenge for the general practitioner (GP) Fear of missing OBD results in many referrals for colonoscopy Colonoscopy is invasive, costly and a scarce resource

introduction Fecal biomarker tests have received much interest Simple, non-invasive tests are needed Fecal biomarker tests have received much interest E.g. calprotectin and iFOB tests

cALPROTECTIN 36 kDa Ca and Zn binding protein mainly derivied from Neutrophies Its belongs to S100 proteins Antimicrobial activity by competing for Zn and by inhibiting of Zn depending enzymes Elevated concentration of it can be measerd in plasma, synovial fluid, urine, liquor, saliva and fecus when an inflammation process with recruitment of neutrophils is ongoing. Ecxellent stability in feces at roon temprature for as long as a week. The units were changed from mg/l to µg/g (feces).

…IN INFLAMMATORY BOWEL DISEASE IBD V/S IBS: sensitivity 95%, specificity: 91% Superior to serological markers such as CRP, ESR, ASCA, p-ANCA. False negative test: delay diagnosis in 6% of adults and 8% of children. False positive: acute gastrointestinal infection and NSAID induced entropathy S100/A12: more specific and sensitive than calprotectin

… in ibd activity IBD activity: symptoms, clinical finding and endoscopy. Calprotectin better than CRP, WBC of blood, clinical activity scores

Correlation with ibd localization Higher FCC in colonic than ileal crohn s disease patients also in recent studies it is not approved. FCC can not be used as a marker of localization of disease

Response to therapy Available data appear still quite weak to support the role of fecal calprotectin as a promising surrogate marker of mucosal healing reducing the need of endoscopic examination.

Fcc in ibd patients undergoing bowel resection Extensive ileocolic resection: diarrhea , bloating, pain without of recurrence of disease. Recurrence of active disease: pain, diarrhea, fever rectal bleeding. FCC is useful to avoid multiple endoscopic examination however more studies is required.

Fecal calprotectin, MMP-9, and human beta-defensin-2 levels in pediatric ibd.(2013)1 ….measured with ELISA in 110 pediatric patients with IBD (Crohn's disease, n = 68; ulcerative colitis (UC), n = 27; unclassified, n = 15; median age, 14). Calprotectin was the best fecal marker in pediatric IBD. MMP-9 showed almost comparable performance in UC. Fecal HBD-2 did not bring information to the disease characteristics of pediatric IBD patients.

Effectiveness and Cost-effectiveness of Measuring Fecal Calprotectin in Diagnosis of Inflammatory Bowel Disease in Adults and Children.2 compare the cost-effectiveness of measuring FC before endoscopy examination with that of direct endoscopic evaluation alone. If endoscopic biopsy analysis remained the standard for diagnosis, direct endoscopic evaluation would cost an additional $18,955 in adults and $6250 in children to avoid 1 false-negative result from FC screening. Screening adults and children to measure fecal levels of calprotectin is effective and cost-effective in identifying those with IBD.

Conclusions Good diagnostic precision for separating organic and functional intestinal disease. (avoid unnecessary endoscopy). A marker of neutrophilic intestinal inflammation, not organic intestinal disease. Better correlate with IBD activity rather than relapse, extent, …..

reference PMID: 24077667 PMID: 23883663