Tittle: Endothelial progenitor cells as a possible component of stem cell niche to promote self-renewal of mesenchymal stem cells Journal name ： Molecular and Cellular Biochemistry Hongwei Zhang, 1,2 Zhiyi Lin, 3 Chaozhe Yang, 4 Meng Zhang, 3 Wenlei Feng, 3 Xinyu Peng, 2 Xueling Chen, 5,* Xiangwei Wu 2,* 1 Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei , China. 2 Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang , China. 3 First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang , China. 4 Children’s National Medical Center, 111 Michigan Avenue NW, Washington, DC 20010, USA. 5 Department of Immunology, School of Medicine, Shihezi University, Shihezi, Xinjiang , China. *First correspondence should be addressed to X.W. and X.C.

CD133 EPCs-PKH26 Suppl Fig. a b c SSC-A FSC-A 48 h CD11b CD31 CD144 Fig. Isolation and Characterization of mouse bone marrow EPC. a CD133 (+), CD31 (+), CD144 (+), CD11b (-) EPCs were isloated from 6-week old male C57BL/6 mice bone marrow by fluorescence- activated sorting (FACS) system. b EPCs were labeled by PKH26, the red fluorescent cell linker. Scale bar: 50μm ； c Tube formation by EPCs on matrigel. PKH26 labeled EPC seeded in 24-well plates coated with Matrigel (1 × 10 5 cells/well) in mEPC culture medium. After 24h or 48h culturing, they were fixed by 4% PFA and examined using fluorescence microscopy. Scale bar: 100μm 24 h

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