Chapter 3.2 and 3.3: Peptides, Proteins, and Working with Proteins CHEM 7784 Biochemistry Professor Bensley.

Slides:

Advertisements

Similar presentations

Electrophoresis Theory

Advertisements

Amino Acids and Proteins B.2. there are about 20 amino acids that occur naturally they are the basic “building blocks” of life/proteins.

Review: Amino Acid Side Chains Aliphatic- Ala, Val, Leu, Ile, Gly Polar- Ser, Thr, Cys, Met, [Tyr, Trp] Acidic (and conjugate amide)- Asp, Asn, Glu, Gln.

Protein Purification and Analysis Day 4. Amino Acids, Peptides, and Proteins.

GEL ELECTROPHORESIS: The method for separation and purification of nucleic acids and proteins.

Introduction to Molecular Biology zMolecular biology is interdisciplinary (biochemistry, genetics, cell biology) zImpact of genome projects (human, bacteria,

Biology 107 Macromolecules II September 5, Macromolecules II Student Objectives:As a result of this lecture and the assigned reading, you should.

Section B: Protein StructureYang Xu, College of Life Sciences Section B Protein Structure B2 Protein structure B3 Protein Analysis.

Chapter 3-Contd. Western blotting & SDS-PAGE

Amino Acids C483 Spring Amino Acid Structure Alpha carbon Sidechain Proteins peptides.

Amino Acids, Peptides and Proteins

Quality Control of Product

Introduction to Molecular Biology zMolecular biology is interdisciplinary (biochemistry, genetics, cell biology) zImpact of genome projects (human, bacteria,

Protein Electrophoresis BIT 230. Electrophoresis Separate proteins based on Size (Molecular Weight - MW) SDS PAGE Isoelectric Point Isoelectric focusing.

BIOCHEMISTRY. PROTEINS Natural polymers C,H,O,N,(S) Monomer is Amino Acids Growth Repair Hormones Enzymes Antibodies Energy Source.

Biomolecules: Peptides and Proteins Lecture 5, Medical Biochemistry.

Gel Electrophoresis Do you want a footer?.

Chemistry 4010 Lab It’s all about PROTEINS… It’s all about PROTEINS…

1 SURVEY OF BIOCHEMISTRY Proteins and Biomolecular Stability.

18.7 Isolation, Purification, and Fractionation of Proteins (1)

CHAPTER 3 Macromolecules: Their Chemistry and Biology

Chapter 4-1 Chromatography

Chapter Five Protein Purification and Characterization Techniques

Biochemistry February Lecture Analytical & Preparative Protein Chemistry II.

Electrophoresis PAGE Dr Gihan Gawish.

Table 5-1 Protein Purification Essential for characterizing individual proteins (determining their enzymatic activities, 3D structures, etc.) Two main.

CHAPTER 3 Amino Acids, Peptides, Proteins Structure and naming of amino acids Structure and properties of peptides Ionization behavior of amino acids and.

Protein Structure & Function Presented By: Shyla Neher February 4, 2004.

Analysis of Proteins and Peptides Amino acid composition Molecular weight Isoelectric point Subunit structure Prosthetic groups Solubility Biological activity.

5. SEPARATION AND DETECTION OF PROTEINS II SDS-PAGE Jana Vobořilová, Anna Kotrbová-Kozak, Vlasta Fürstová, Tereza Kopská.

BIOCHEMICAL METHODS USED IN PROTEN PURIFICATION AND CHARACTERIZATION

Protein Extraction, Fractionation, and Identification

Amino Acids and Proteins B.2. Properties of 2-amino acids (B.2.2) Zwitterion (dipolar) – amino acids contain both acidic and basic groups in the same.

Structure of proteins Vladimíra Kvasnicová. Chemical nature of proteins biopolymers of amino acids macromolecules (M r > )

ERT106 BIOCHEMISTRY PROTEIN TECHNOLOGY By Pn Syazni Zainul kamal.

Electrophoresis Defined as the migration of charged particles through a solution under the influence of an electric field. Many important biological molecules.

Amino acids/Proteins.

Separation of main plasma protein by using SDS-PAGE

SDS-Polyacrylamide Gel Electrophoresis

Preparing yeast cell extracts SDS-PAGE gives a snapshot of proteins in an extract Proteins are extracted from cells.

Analysis of amino acids and proteins

Proteomics The science of proteomics Applications of proteomics Proteomic methods a. protein purification b. protein sequencing c. mass spectrometry.

Exam I Review I. Several Amino Acids Occur Rarely in Proteins Figure 4.4 (c) Several amino acids that act as neurotransmitters and hormones.

AP Biology Discuss the following with your group and be prepared to discuss with the class 1. Why is the shape of a molecule important? 2. How is a covalent.

PROTEIN TECHNOLOGY By DR ZARINA ZAKARIA. Why to exploit protein Information about protein structure has led to a deeper understanding of the evolutionary.

Separation techniques ?. Molecules can be separated: Chemically: by charge, by action with specific reagents Physically: by solubility, by molecular weight,

SDS-PAGE Ms. Nadia Amara.

Amino Acids & Proteins The Molecules in Cells Ch 3.

General Tests for Chemistry 101

Polyacrylamide Gel Electrophoresis. Electrophoresis Horizontal Agarose Gels Agarose forms a gel or molecular sieve that supports the movement of small.

Proteins & its structure. Proteins Proteins are heteropolymers of  -amino acids Amino acids have properties that are well suited to carry out a variety.

Proteomics For any given species, the space of possible biomolecules and their organization into pathways and processes is large but finite.

생화학 (BIOCHEMISTRY) 제 4 주차 생명환경과학대학 김 창 규.

Objectives Describe the chemical composition and general structure of carbohydrates. Describe three classes of carbohydrates, how they are synthesized,

Tymoczko • Berg • Stryer © 2015 W. H. Freeman and Company

Quality Control of Product

ELECTROPHORETIC METHODS

Pratt & Cornely Chapter 4

Sodium Dodecyl Sulfate -Polyacryl Amide Gel Electrophoresis [SDS-PAGE]

Protein Proteins are biochemical compounds consisting of one or more polypeptides typically folded into a globular or fibrous form in a biologically functional.

Amino Acids, Peptides, and Proteins

Chapter 5 Proteins.

Chapter 5. Protein Purification and Characterization Techniques

SDS-Polyacrylamide Gel Electrophoresis Agarose vs. SDS-PAGE

Column Chromatography

Analytical & Preparative Protein Chemistry I

Electrophoresis / SDS-PAGE

CHAPTER 2 Amino Acids, Peptides, Proteins

Sodium Dodecyl Sulfate -Polyacryl Amide Gel Electrophoresis [SDS-PAGE]

Sodium Dodecyl Sulfate -Polyacryl Amide Gel Electrophoresis [SDS-PAGE]

Presentation transcript:

Chapter 3.2 and 3.3: Peptides, Proteins, and Working with Proteins CHEM 7784 Biochemistry Professor Bensley

CHAPTER 3.2 and 3.3 Peptides, Proteins, and Working with Proteins The structure and properties of peptides The ionization behavior of and peptides Various methods to characterize peptides and proteins Today’s Objectives: to understand

Formation of Peptides Peptides are small condensation products of amino acids They are “small” compared to proteins (M w < 10 kDa)

Peptide Ends are Not the Same Numbering starts from the amino terminus AA 1 AA 2 AA 3 AA 4 AA 5

The Three Letter Code Naming starts from the N-terminus Sequence is written as: Ala-Glu-Gly-Lys Sometimes the one-letter code is used: AEGK

Peptides: A Variety of Functions Hormones and pheromones Neuropeptides Antibiotics Protection, e.g. toxins

Proteins are: Polypeptides (covalently linked  -amino acids) + possibly: cofactors coenzymes prosthetic groups other modifications

Polypeptide Size in Some Proteins

Classes of Conjugated Proteins

What to Study about Peptides and Proteins? What is its sequence and composition? What is its three-dimensional structure? How does it find its native fold? How does it achieve its biochemical role? How is its function regulated? How does it interact with other macromolecules? How is it related to other proteins? Where is it localized within the cell? What are its physico-chemical properties?

A Mixture of Proteins Can Be Separated Separation relies on differences in physico-chemical properties –Charge –Size –Affinity for a ligand –Solubility –Hydrophobicity –Thermal stability Chromatography is commonly used for preparative separation

Column Chromatography

Separation by Charge

Separation by Size

Separation by Affinity

Electrophoresis for Protein Analysis Separation in analytical scale is commonly done by electrophoresis –Electric field pulls proteins according to their charge –Gel matrix hinders mobility of proteins according to their size and shape

SDS PAGE: Molecular Weight SDS – sodium dodecyl sulfate – a detergent SDS micelles bind to, and unfold, all the proteins –SDS gives all proteins an uniformly negative charge –The native shape of proteins does not matter –Rate of movement will only depend on size: small proteins will move faster