Thin Layer Chromatography bsapp.com. Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line.

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Presentation transcript:

Thin Layer Chromatography bsapp.com

Preparing a Sample Space Cut a sheet of paper so it may hang free within the intended container Mark a sample line ½ inch to an inch from the bottom bsapp.com

Place your samples on the sheets bsapp.com

Starting the Sample Place the sheet in a container with a solvent The bottom of the paper should be submerged into the solvent, but not above the samples bsapp.com

Allow your samples to run at least ½ way up the sample space bsapp.com

bsapp.com

Allow your samples to drybsapp.com

Matching Samples Note component colors Calculate Rf values bsapp.com

Calculating Rf Values Rf value is a ratio of distance a component sample moves to the distance the solvent moves This number is usually expressed as a decimal bsapp.com

Calculating Rf Values  Starting point  8 cm  4 cm  Solvent finish 10 cm Rf = 4/10 = 0.4 Rf = 8/10 = 0.8 bsapp.com