Supplementary material: Applied Microbiology and Biotechnology Proliferation of diversified clostridial species during biological soil disinfestation incorporated with plant biomass under various conditions Subrata Mowlick a, Toshiaki Takehara b, Nobuo Kaku a, Katsuji Ueki a, and Atsuko Ueki a a Faculty of Agriculture, Yamagata University, Yamagata, Japan b NARO Western Region Agricultural Research Center, Hiroshima, Japan Corresponding author: Dr. Atsuko Ueki Faculty of Agriculture, Yamagata University, 1-23, Wakaba-machi, Tsuruoka , Yamagata, Japan. Tel.: ; Fax: address:

Denaturing gradient 60 % 30 % Y2 Q2 A1 G2 L1 U1 E1 K1 P2 (a) (b) Supplementary Fig A1 G2 K1 E1 Y2 U1 Q2 L1 P2 Supplementary Fig. 1. (a) DGGE patterns for the original (non-treated) and the treated soil samples. (b) Cluster analysis of DGGE band pattern for similarity index. The lane Y2 indicates for the original soil, whereas other lanes for the treated soil at 30 o C under different MC: control (Q2 for 20%, U1 for 30%), Brassica-treated (A1, E1) wheat bran-treated (G2, K1), and Avena-treated soils (L1, P2). Other notifications of DGGE were similar as described in Fig. 1 of the main text.

Number of clostridial clones Supplementary Fig A1 K1 E1 H1 Number clostridial of OTUs Supplementary Fig 2. Rarefaction curves for the clostridial groups of the libraries of soils that effectively suppressed the pathogen. Clone library: A1, Br30-20; E1, Br30-30; H1, Wh25-30; K1, Wh30-30.

Soil sampleA1E1H1K1 No. of total clostridial clones No. of total clostridial OTUs Coverage (%) Chao1 (richness) Shimpson's diversity index Shannon-Wiener diversity index Supplementary Table 1. Estimate of richness and diversity of clostridial community for the soil samples suppressed the pathogen effectively Soil sample: A1, Br30-20; E1, Br30-30; H1, Wh25-30; K1, Wh30-30.