B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z 760.6 Figure 1 300.

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B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z Figure 1 300

x Figure A. Total ions (Control Hippocampus) B. Ischemic Hippocampus Signal Intensity m/z m/z 760.6

1500 B. MALDI IMS Ischemic Hemisphere A.MALDI IMS Control Hemisphere m/z Figure 3

Figure 4 A.TTC Stained Rat Brain Slice 550 B. 16:0/18:1 PC [M+H] + m/z C. 16:0/18:1 PC [M+Na] + m/z D. 16:0/18:1 PC [M+K] + m/z 798.6

m/z 100 Relative Intensity B. CID (pos ion) m/z A. MALDI IMS of Rat Brain 96 hours post TBI m/z Figure 5

Supplemental Figure m/z CID m/z Cer 18:1/ 13 C 4 -16:0 [M+H] + -H 2 O Relative Intensity 100 [Cer(d18:1/ 13 C 4 -16:0)-H 2 O] m/z ESI/MS POPC-H + POPC-Na + Cer(d18:1/ 13 C-16)-H 2 O + Cer(d18:1/ 13 C-16) +