Results: / In Vitro Biocompatibility of Iron Filled Carbon Nanotubes Taylor,

Slides:

Advertisements

Similar presentations

Institut für Mineralogie Detection and Imaging by Electron Microscopy Investigations by using electron microscopy offer the possibility to detect and image.

Advertisements

Cytotoxicity and Uptake of Nanoparticles in Cells Courtney Rister Faculty Advisors: Virginia Ferguson Conrad Stoldt Department of Mechanical Engineering.

THE EFFECT OF TYPE OF NANOPARTICLES ON THE QUENCHING PROCESS

Enhancement of Phenol Biodegradation by South Magnetic Field Exposure Jongtai Jung (Professor/Ph. D) (Professor/Ph. D) Major of Environmental Engineering.

Human Papillomaviruses (HPV) such as HPV16 and HPV18 can induce cervical cancer. In this case, the two HPV E6 and E7 oncoproteins are essential players.

Theoretical Analysis of a Nanosensor based on WO 3 coated Carbon Nanotube for Ultra-high Sensitive Breath Acetone Sensing Ming Xia, Advisor: Prof. Xingguo.

POTENTIAL ROLE OF CYTOCHROME P450 3A4 (CYP3A4) IN THE PCB104-MEDIATED BARRIER DYSFUNCTION OF HUMAN MICROVASCULAR ENDOTHELIAL CELLS Yong Woo Lee 1, Sung.

PREPARATION OF ZnO NANOWIRES BY ELECTROCHEMICAL DEPOSITION

Chapter 4 Introduction to Nanochemistry. 2 Chapter 4 Periodicity of the Elements Chemical Bonding Intermolecular Forces Nanoscale Structures Practical.

Photo-therapy K.O. Lenz BMS Wilmington, DE. Photo-therapy.

Porous PEG-Fibrinogen Hydrogel Scaffolds for Tissue Engineering Ortal Yom-Tov The Interdepartmental Program for Biotechnology Technion Supervisors: Prof.

 The way in which nanotubes are formed is not exactly known. The growth mechanism is still a subject of controversy, and more than one mechanism might.

#627: Multitarget gene inhibition by synthetic nucleic acids in bladder cancer cells Burmeister Y, Kraemer K, Fuessel S, Kotzsch M #, Meye A*, Hakenberg.

Supermacroprous chitosan–agarose–gelatin cryogels: in vitro characterization and in vivo assessment for cartilage tissue engineering by Sumrita Bhat, Anuj.

SiRNA-mediated Down-Regulation of Survivin Inhibits Bladder Cancer Cell Growth S. Fuessel, S.Ning, M. Kotzsch #, K. Kraemer, M. Kappler*, U. Schmidt, H.

CYTOTOXICITY OF SILVER SULFIDE QUANTUM DOTS EVALUATED BY THE MTT CELL CULTURE ASSAY in HeLa CELLS Deniz Özkan Vardar 1, Ibrahim Hocaoğlu 2, Havva Funda.

Dipl. Chem. Mark Geppert Center for Biomolecular Interactions, University of Bremen, Germany Center for Environmental Research and Sustainable Technology,

Nanotechnology in Cancer Treatment

Title: Anisotropic Colloidal Magnetic Nanostructures NSF Award Numbers: # Recent years have witnessed a remarkable convergence of physical sciences,

PROPERTIES OF CARBON NANOTUBES

Figure 3. SEM images of electrospun microfiberous scaffolds : (A) random PCL scaffold and (B) highly aligned PCL scaffold. Analysis of orientation with.

The Effects of Stress on Post-Natal Stem Cell Proliferation and Differentiation Robert McKinstry Pittsburgh Central Catholic High School.

Heat Shock Protein 90 (HSP90) is over-expressed in p16 negative oropharyngeal squamous cell carcinoma and its inhibition in vitro potentiates the effects.

The Use of a Vesicular Delivery System in the Enhancement of Cisplatin Bioavailability Manal M. Alsaadi, Katharine C. Carter and Alexander B. Mullen Strathclyde.

Cellular Toxicity of Carbon - based Nanomaterials Nano Letters, 2006, 6 (6), pp 1121–1125 Electrical Engineering and Computer Sciences University of California,

Uptake studies of FITC-labelled siRNAs in Uptake studies of FITC-labelled siRNAs in subcutaneous EJ28 xenografts Doreen Kunze 1, Daniela Wuttig 1, Ingo.

DIESEL EXHAUST PARTICLES and SERUM MODULATE AIRWAY EPITHELIAL CELL VIABILITY by AFFECTING INTRACELLULAR CELL SIGNALING PATHWAYS, WHICH ARE SENSITIVE to.

Effect of High Concentrations of Diesel Exhaust Particles on Human Lung Epithelial Cell Viability and Death Hasan Bayram 1*, Kazuhiro Ito 2, K. Fan Chung.

Inhibition of C13orf19 mRNA expression by siRNA in prostate cancer cells * Introduction A high proportion of bladder cancer.

Discussion The C13orf19 mRNA inhibition by D5 has no effects on cellular growth properties. We suppose that the inhibition leads to reduced apoptosis in.

22 October 2014 Claudia Moia Early Stage Researcher, Cranfield University (UK) Cell type- and size- dependent in vitro toxicity of silica particles in.

1 Carbon Nanotube In Biology Lawanya Raj Ojha Graduate Student Department of Chemistry, OSU, Stillwater.

Large Scale Synthesis of Near-Monodisperse Gold Nanorods and their Assembly into 3D Anisotropic Single Crystals Eugene R. Zubarev, William Marsh Rice University,

PURPOSE The purpose of this work was to commission and to evaluate the role of MR guided high intensity focused ultrasound (MRgHIFU) in cancer therapy.

Supplementary Figure 1: Silibinin has no considerable radiosensitizer effect on radioresponsive prostate cancer 22RV1 cells. (A) Effect of IR and SB on.

Supplementary Material Human Proximal Tubule Cells Form Functional Microtissues Jenny A. Prange 1, Manuela Bieri 1, Stephan Segerer 2, Charlotte Burger.

Introduction. During the last decade the interest in copper passivity significantly increased due to the important role of copper in microelectronic industry.

Supplementary Figure 1. The effect of 17-DMAG on the growth of lung cancer cells with Met amplification Tumor cells were continuously treated with increasing.

Gene therapy and Viral Vectors Lecture 6. Contents Introduction to gene therapy Delivery of Therapeutic Genes Gene Therapy Targets Delivery Modes Steps.

PROTEASOME INHIBITION AND NOT NF-ΚB INHIBITION INDUCES APOPTOSIS TO RESISTANT CELLS IN GLUCOCORTICOID-INDUCED APOPTOSIS George I Lambrou 1, Apostolos Zaravinos.

6 th World Congress on Biotechnology Leaves extract of Damdei, Lamka for the synthesis of mixed oxide of Zn nanoparticles: Truly biogenic method Presented.

Adenosine Protects Vascular Barrier Function in Hyperoxic Lung Injury Jonathan Davies 1, Harry Karmouty-Quintana 2, Thuy T. Le 2, Ning-Yuan Chen 2, Tingting.

Date of download: 6/21/2016 Copyright © 2016 SPIE. All rights reserved. Schematic diagram of thermal destruction and photothermal treatment (PTT) study.

Targeting of reactive oxygen species can be a potential therapeutic strategy for cancer treatment Ying-Ray Lee 1, San-Yuan Chen 2, and Hau-Ren Chen 3 1.

Synthesis and Characterization of Magnetic ` Chitosan Microspheres for Medical Applications ` Sang Gil Ko 1 *, Jun Hee Cho 1, Yang kyu Ahn 1, Ki Chang.

多肽类药物研究的新进展潘婷婷多肽药物定义：通常将含有的氨基酸少于 10 个的肽称为寡肽，超过的就称为多肽。所以多肽药物可以这样说：从生物化学本质上说是一种肽，具有 10 个氨基酸以上；从功能上讲具有药物的功能，能用于疾病的预防、治疗与诊断。

Investigation of the effect of Thymoquinone (TQ) alone or in combination with cisplatin on cell growth, cell cycle progression and apoptosis of human oral.

AP Lab 5 Cellular Respiration.

2Institute of Ceramics and Glass-CSIC, Madrid, Spain

Nucleate pool boiling heat transfer of TiO2–R141b nanofluids

INCREASING CELL VIABILITY USING DOUBLE HYDROXIDE MATERIALS FOR BIOLOGICAL LABELING I.Castelló Serrano,1,* G. Stoica,2 E. Palomares2.

University of Leicester

A B C D Figure S3: DiO6(3) staining of mitochondrial networks in primary prostate tumor organ cultures. (A) Epithelial.

Neuroprotective Effect of Didymin on Hydrogen Peroxide-Induced Injury in the Neuronal Membrane System Cells Tissues Organs 2014;199: DOI: /

Magnetic nanoparticles as a new approach to improve the efficacy of gene therapy against differentiated human uterine fibroid cells and tumor-initiating.

BTY100-Lec 2.3 Nanobiotechnology.

Hailong Zhang, Wei Zhang, Yong Zhou, Yuhua Jiang, Shupeng Li

Near Infrared Imaging and Photothermal Ablation of Vascular Inflammation Using Single-Walled Carbon Nanotubes by Hisanori Kosuge, Sarah P. Sherlock, Toshiro.

Introduction - characterization of materials.

Fluid Phase Endocytosis Contributes to Transfection of DNA by PEI-25

Magnetic nanoparticles as a new approach to improve the efficacy of gene therapy against differentiated human uterine fibroid cells and tumor-initiating.

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor Hiroshi Ishihara, Hiroshi Yoshimoto,

Hailong Zhang, Wei Zhang, Yong Zhou, Yuhua Jiang, Shupeng Li

Ellagic and Tannic Acids Protect Newly Synthesized Elastic Fibers from Premature Enzymatic Degradation in Dermal Fibroblast Cultures Felipe Jimenez,

Nitric oxide from rat liver sinusoidal endothelial cells induces apoptosis in IFN γ- sensitized CC531s colon carcinoma cells Katrien Vekemans, Filip Braet,

Synthesis of Functional Proteins by Mixing Peptide Motifs

Molecular Therapy - Nucleic Acids

Figure 1. Effects of Gal-4 on apoptosis and proliferation of monocytes

FACS analyses of the inhibitory effect induced by mixed docetaxel, gefitinib, and cyclopamine on EGF plus SHHNp–stimulated PC3 cells. FACS analyses of.

Aminoglycoside Enhances the Delivery of Antisense Morpholino Oligonucleotides In Vitro and in mdx Mice Mingxing Wang, Bo Wu, Sapana N. Shah, Peijuan.

Presentation transcript:

Results: / In Vitro Biocompatibility of Iron Filled Carbon Nanotubes Taylor, A., Krämer, K. 1, Hampel, S., Füssel, S. 1, Klingeler, R. 2, Ritschel, M. 2, Büchner, B. 2, Wirth, M. P. Taylor, A. 1, Krämer, K. 1, Hampel, S. 2, Füssel, S. 1, Klingeler, R. 2, Ritschel, M. 2, Büchner, B. 2, Wirth, M. P Department of Urology, Faculty of Medicine, Dresden University of Technology, Germany 2. Leibniz Institute for Solid State and Materials Research (IFW) Dresden, Germany. Introduction & Objectives: Magnetic Fluid Hyperthermia is a potential therapy for treating cancer. Most of current research is focused on nanoparticles made of iron oxides since other magnetic materials contain potentially toxic elements. Metallic iron could enhance the heating effect. Its application is however hindered because of oxidation in ambient or biological conditions. Carbon nanotubes (CNTs) can overcome this problem, as a protective coating for iron. Furthermore, they could be explored as multi-functional containers carrying not only iron but other materials and/or molecules of interest (Figure 1).  Aim of this study: explore the biocompatibility of iron-filled carbon nanotubes in comparison to empty ones. Materials & Methods: Iron filled carbon nanotubes were synthesized by catalytic decomposition of ferrocene in a CVD system and characterized by TEM, X-Ray diffraction and SQUID magnometry. In order to suspend the nanotubes in aqueous solutions, an ultrasonication procedure in a PBS solution containing albumin in a 1:1 weight ratio in respect to the nanotubes was applied. Cellular uptake was evaluated by transmission electron microscopy in bladder (EJ28) and prostate cancer cells lines (PC-3) and non-malignant fibroblasts. Toxicity was studied in a time (24h, 48h and 96h) and concentration dependance (6.2, 25 and 100 µg/ml) using a prostate cancer cell line (PC-3) and a non-malignant cell lineage (fibroblasts). Effect of nanotubes was evaluated by measurement of: metabolic activity (WST-1 assay), cell proliferation (cell counting), apoptosis rate (Annexin-V/PI labelling and FACS analysis) and cell cycle distribution (PI staining and FACS analysis). Conclusions: Cells were not significantly affected by exposure to empty or iron filled carbon nanotubes to concentrations up to 100 µg/ml. These results indicate that the iron filling is well encapsulated inside of the nanotubes, leading to no major toxic effects. Further studies will focus on the heating efficieny of cells and tissues incubated with iron filled CNTs. Figure 1. Schematic representation of a multi-functional nanocontainer. CNT filling (magnet, temperature sensor, drugs,...) Targeting/Solubilizing moieties Cell Seeding Incubation with CNTs (4h) Evaluation after 24h Evaluation after 48h Evaluation after 96h Figure 2. Association of iron filled nanotubes with cells as observed by optical and transmission microscopy. Control PC-3 cells (a) and cells exposed to 100 µg/ml of iron filled CNTs for 4h (b). Control EJ28 cells (c) and exposed to 100 µg/ml of empty (d) or iron filled carbon nanotubes (e) for 24h. Higher magnification of iron filled CNT inside of EJ28 cell (f). Figure 3. Relative viability of PC-3 cells after exposure to empty and iron filled CNTs as evaluated by WST-1 assay. Figure 4. Proliferation of PC-3 cells after exposure to empty and iron filled CNTs as evaluated by cell counting. Figure 5. Cell cycle distribution of PC-3 cells after exposure to iron filled CNTs as evaluated by PI staining and FACS analysis. Figure 6. Apoptosis rate of PC3 cells after exposure to iron filled CNTs as evaluated by Annexin-V/PI labelling and FACS analysis. A small concentration dependant decay in viability was observed 24h after incubation with iron filled carbon nanotubes. After 48h, viability was recovered and remained stable up to 72h, when cells reached confluency. Cell proliferation was not impaired by incubation with empty nor filled nanotubes. Cell cycle distribution was identical to control conditions, indicating no adverse effects from the presence of nanotubes. A decrease in the number of cells in the S and G2 phase is observed because confluence is reached. The number of apoptotic cells was comparable to control conditions in all cases, indicating no toxic effects induced by nanotubes. Similar results obtained with fibroblasts, indicating biocompatibility with malignant and non-malignant cell lines. CNT Wall Iron Filling (a) (b) (d) (e) (f) 1,8 µm 0,08 µm 400 × (c) Research was supported in part by the European Network CARBIO, Contract MRTN-CT CNTs were found internalized by cells and also well attached to the cellular membrane.