The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.

The ProteomeWorks System ~ New Mass Spectrometry Systems ~ At Pittcon 2001 Micromass introduced a range of e- MS TM enabled MALDI-TOF MS / MS-MS & ESI LC-MS-MS systems and Mass-Informatics TM Software to leverage The ProteomeWorks TM System HT TM Q-Tof Ultima TM Q-Tof micro TM

Micromass’ new networked MALDI TOF-MS & sample management system “2D-gel-MS analyser for proteomics factories” HT TM [new instrument]

HT TM - Networked MALDI-TOF MS REFLECTRON Peptide Mass Fingerprinting LINEAR Intact Proteins

ELECTRO MAGNETIC GRIPPER TARGET PLATE CASSETTE 2 X - Y ROBOTIC ARM TARGET PLATE CASSETTE 1 HORIZONTAL AXIS MOTOR VERTICAL AXIS MOTOR BAR CODE SCANNER VACUUM LOCK LID IN COLUMN OUT COLUMN VERTICAL FLIGHT TUBE AND REFLECTRON HOUSING HT TM - Sample Management System 80 x 96/384-Sample Targets (bar coded)

HT TM - Networked MALDI-TOF MS Linear and reflectron ion optics options 80 x 96/384-Sample target management system Bar coded sample target plates Automated Laser Positioning System (ALPS) for sample target alignment and sample indexing Intelligent acquisition / calibration algorithm Web-enabled Client-Server Mass-Informatics for interrogation of non-redundant protein dB …converts samples into knowledge not ‘just’ spectra

ProteinLynx TM Global SERVER web-enabled Mass-informatics

ProteinLynx TM Global SERVER export to PDQuest Annotation Tools

PDQuest Annotation Tools C L I C K

MS & MS-MS Techniques for Peptide / Protein Analysis

Q-Tof Ultima TM family [new instruments] DDA TM Micromass’ SuperPremium family of MALDI MS-MS and ESI MS-MS systems for PROTEOMICS and structural analysis

DDA TM Q-Tof Ultima  …the SuperPremium family of MS-MS Q-Tof Ultima  MALDI The high throughput MALDI MS-MS Q-Tof Ultima  API The SuperPremium API LC-MS-MS Q-Tof Ultima  GLOBAL The flexible research platform for API LC-MS-MS / MALDI MS-MS

Q-Tof Ultima TM MALDI [new instrument] Dedicated MALDI MS-MS system V & W- OPTICS options ProteinLynx Global SERVER e-MS enabled

Q-Tof Ultima MALDI with V OPTICS 10,000 FWHM

Q-Tof Ultima MALDI with W OPTICS 20,000 FWHM

MALDI MS-MS is superior to PSD ADH 1 pm/µl PSD ADH 1 pm/µl Q-Tof Ultima MALDI MS-MS

ProteinLynx TM Global SERVER... Matching MS-MS spectra to dB of known protein or nucleotide sequence.

Q-Tof Ultima TM API [new instrument] Dedicated API (ESI) LC-MS-MS system V & W- OPTICS options DDA - Precursor Ion Discovery (patent applied for) – “Neutral Loss” – “Precursor Ion” – High resolution – Exact mass – Compatible with HPLC ProteinLynx Global SERVER e-MS enabled

Q-Tof Ultima API with W OPTICS 20,000 FWHM

Q-Tof Ultima TM API...comparison of V & W- OPTICS Resolution

The superior peptide sequence coverage provided by ESI MS-MS data enables de novo sequencing

Sequencing results from MS-MS of m/z 841.5

Q-Tof Ultima TM GLOBAL [new instrument] Flexible API & MALDI MS-MS research platform API / MALDI changeover without venting V & W- OPTICS options LC-DDA-MS – Precursor ion discovery – “Neutral Loss” – “Precursor Ion” – Exact mass ProteinLynx Global SERVER e-MS enabled

Q-Tof Ultima GLOBAL

Q-Tof micro TM [new instrument] Micromass’ finest API Q-Tof technology for structural analysis & protein characterisation … SMALLER SMARTER WIRED DDA TM

Q-Tof micro TM … SMALLER Z SPRAY TM nanoflow ESI inlet technology 5000 FWHM resolution 5ppm RMS exact mass measurement Dynamic External Calibration (DXC TM ) m/z range

Q-Tof micro TM …SMARTER …WIRED Data Directed Analysis (DDA) – Automates analysis of protein digests by LC-DDA-MS Precursor Ion Discovery (patent applied for) – “Neutral Loss” – “Precursor Ion” – High resolution – Exact mass Compatible with HPLC Maximising data quality

Data Directed Analysis DDA TM Exclusion of 1 + background ions

The New e-MS TM Enabled Mass Analysers of The ProteomeWorks TM System... msinnovation.com

The ProteomeWorks System is the global alliance of Micromass and Bio-Rad Laboratories dedicated to furthering proteomics research.