Staining HAEMATOXYLIN & EOSIN

Slides:

Advertisements

Similar presentations

Gross Techniques In Surgical Pathology. Introduction The routine work associated with a surgical pathology specimen includes gross & microscopic examinations.

Advertisements

Characterization of ET1ep-/- mouse line

Immunohistochemistry ESE3 Immunohistochemistry. stained prostate tissue samples for ESE3 troubleshooted ESE3 antibody using the controls - no antibodies.

Types of microscopes & Microtechniques.

S T A I N I N G DWI WINARNI Departemen Biologi Fakultas Sains dan Teknologi Universitas Airlangga Surabaya.

Dr Vivien Rolfe De Montfort University This is an Open Educational Resource (OER) that is globally available on the web Creative Commons BY SA.

Dr Vivien Rolfe De Montfort University This is an Open Educational Resource (OER) that is globally available on the web Creative Commons BY SA.

Artifacts in Histological Preparations. Microwave heating It’s a kind of low power energy carried by micro-waves Internal direct heating is our advantage.

Immunohistochemistry

Histology Instructors: Faris Mohammednoor Altaf MT. MS. Ph.D. د. فارس محمد نور سعيد ألطف Phone# ext

Dr. Samah Kotb Nasr Eldeen. Biological stains are prepared from dyes which have been manufactured to rigid specifications; to ensure that they are suitable.

Dr. Samah Kotb Nasr Eldeen.  Several types of staining processes are used to color tissues for microscopical examination.  Staining methods depend.

PREPARATION OF HISTOLOGICAL SPECIMENS

Observation of bacteria using staining procedures Simple staining Gram staining.

CYTOLOGY BY Dr. TAREK ATIA Histology and Cell Biology.

Histological Stains. Tissue Processing To some extent, light-level (as opposed to e.m.- level) histology is a lost art, especially if one is interested.

PATHOPHYSIOLOGY PATHOPHYSIOLOGY w DEFINED Involves the study of function that results from disease processes.

Histology and Embryology 组织学与胚胎学 Department of Histology and embryology Three Gorges University, Yichang, China.

Staining of Bacteria Staining of Bacteria Brno University of Technology, Faculty of Chemistry Hana ŠURANSKÁ.

+ Research Techniques I (Biology 513) Staining. + Why stain tissue? Most tissues are colorless and unless one uses diffraction interference contrast microscopy,

1- Histology and Histo-technique

Histology Histology is the study of the tissues of the body and how these tissues are arranged to constitute organs Literally, histology means tissue or.

Dr. Samah Kotb 2015 Histology Techniques CLS 322.

Tissue Processing Dr : Hala El-sayed Mahmoud

PREPARATION OF HISTOLOGICAL SPECIMENS

Histology and Embryology

The Process Of Molecular Cytology: Embedding and Sectioning Natasha Williams Dr. Katia Manova Zuckerman Research Building/ MSKCC.

Light Microscope. Light Microscope Light Microscope The light microscope depends on light passing through an object in order for it to be seen. The result.

Lab #6: Immunohistochemistry (IHC)

Histology & Its Methods of Study 2015/16 1Lufukuja G.

TISSUE PREPARATION.

Stains of Blood Films “ Leishman Stain “.

Lab 4 & 5 Staining Technique

Tissue processing & sectioning. Biopsy: examination of tissue taken from living body (gross µscopical examination). Autopsy: examination of dead.

H&E STAINING Lab Lec # 4.

Course Descriptive Histology Introduction.

THE GRAM STAIN. The gram stain is the most widely used staining procedure in bacteriology It is called a differential stain it differentiates between.

Preparation of tissues for study

Professor KI-JONG RHEE Teaching assistant MIN HO LEE.

Electron Microscopy 7th lecture.

Preparation of Plant tissues for histological study

 The sections, as they are prepared, are colourless and different components cannot be appreciated.  Staining them by different coloured dyes, having.

Histological techniques: Haematoxylin and Eosin staining

Elastica van Gieson-picro-fuchsin staining

Histology introduction

Histological Techniques

Pathophysiology.

بسم الله الرحمن الرحيم Department of Pathology College of Medicine

Lab 4 & 5 Staining Technique

Principles of Laboratory Diagnosis of Infectious Diseases

SPECIAL STAINS FOR NUCLEIC ACID.

بسم الله الرحمن الرحيم Department of Pathology College of Medicine

College of Education Biology Dept.

Bacterial Cell Walls.

THE GRAM STAIN.

Faculty of Medicine and Health Sciences

Staining Methods.

Topic 1: Introduction to Histology

Cell Biology 3 - Tools of the cell biologist

Published in Thyroid. January 2014, 24(1): DOI: /thy

General Principles of Tissue Preparation and Staining

Methods of Sample Preparation

Lab 4 & 5 Staining Technique

Histology and Embryology

Immunohistochemistry

Interpretation of Histological sections

Practical of Histopathology

Presentation transcript:

Staining HAEMATOXYLIN & EOSIN Supervised by : Dr.Sherifa Prepared by: Reem Aldossari Lama Alabdi

H&E staining: The best known and most widely used staining procedure, hematoxylin-eosin staining (H&E), uses hematoxylin solutions for nuclear staining and eosin solutions for cytoplasmic staining. In the first step, the nuclei are stained with a hematoxylin solution. The nuclei stain blue, dark violet to black. The second step is counterstaining with eosin Y or eosin B. In this process cytoplasm, collagen, keratin stain red.

H&E staining is the standard staining method used in histology H&E staining is the standard staining method used in histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal, inflammed ,degenerative and pathological changes . It is often possible to make a diagnosis based on the results of H&E staining, or it can be determined which special staining techniques are still necessary for subtyping.

APPLICATION Gives a general overview of the tissue structure. Can be used as a diagnostic tool.

Advantages of H&E staining Combination of the two stains gives outstanding results. Ready to use reagents . Easy to use. Results in just two steps.

Procedure for H & E Staining of Paraffin Sections: 1-(Fixative: Any general fixative is adequate. Ex.formalin) 2--Deparaffinization in : 3 - labeled Xylene I ( 1-3 minutes) 4- EtOH (ethanol alcohol) 100% (2-3 minutes 5-EtOH(ethanol alcohol) 96% (2-3 minutes) 6-EtOH(ethanol alcohol) 80% (2-3 minutes) 7-EtOH(ethanol alcohol) 70% (2-3 minutes) 8-Dist. water, 2 minutes. 9. Hematoxylin, approximately 10 minutes. 10-EtOH(ethanol alcohol) 70% (2-3 minutes) 11-EtOH(ethanol alcohol) 80% (2-3 minutes) 13-Eosin Y solution, 2 to 5 minutes. 16- Absolute 100% ethanol, 1-2 minutes. 18-Xylene I, 1-2 minutes. 21-Mounting with DPX 12-EtOH(ethanol alcohol) 96% (2-3 minutes)

Thank you