LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc.

LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc. Make light work of metabolite ID The result—faster, more comprehensive metabolite identification. Now the metabolite identification workflow is even easier and more efficient with automated method development. Designed by customers with input from pharmaceutical companies worldwide. Specifically designed workflow for the Met ID lab Automated method builder creates a full set of optimal IDA methods on the QTRAP ® System Auto-built expert-level methods provide increased metabolite detection within a single IDA analysis Auto-built “pMRM” methods increase metabolite detection significantly over traditional strategies Intelligent application and automation reduce the need for extensive QTRAP ® System operator training AB SCIEX Drug Discovery Solutions for Metabolite Identification

LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc. AB SCIEX Drug Discovery Solutions for Metabolite Identification Comprehensive set of features makes light work of metabolite ID Acquire MRM Acquire MRM Acquire Precursor Acquire Precursor Acquire Neutral Loss Acquire Neutral Loss Acquire EMS Acquire EMS LightSight™ Software Metabolite Search LightSight™ Software Metabolite Search ACD/MS Processor ACD/MS Processor Report

LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc. Make light work of metabolite ID AB SCIEX Drug Discovery Solutions for Metabolite Identification

LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc. LightSight ™ 2.0 Software automates Predictive MRM (pMRM) MRM is the next generation of theoretically predicted metabolite MRMs for Information Dependent Acquisition (IDA) Also predicts MRM transitions for metabolites that are a result of combined transitions Biotransformation List Predicted Fragments + Automated Method Built With pMRMs IDA collects MS/MS data to confirm metabolites AB SCIEX Drug Discovery Solutions for Metabolite Identification

LightSight ™ 2.0 Software © 2008 Applera Corporation and MDS Inc. GSH conjugated metabolites indicates potential reactive metabolites LightSight ™ Software Automates Glutathione Detection to Make light work of metabolite ID Reactive/electrophilic metabolites conjugate with the tri-peptide Glutathione Only the QTRAP ® System allows selective HT GSH screening with confirmation Gly Cys Glu Three Automated Methods for finding Glutathione using LightSight ™ Software: ›Negative Precursor 272 ›Positive Neutral Loss 129 ›pMRM with the GSH only biotransformation set Three Automated Methods for finding Glutathione using LightSight ™ Software: ›Negative Precursor 272 ›Positive Neutral Loss 129 ›pMRM with the GSH only biotransformation set Metabolite Profiling is efficient when Combining LightSight 2.0 Software And the powerful QTRAP ® System Metabolite Profiling is efficient when Combining LightSight 2.0 Software And the powerful QTRAP ® System AB SCIEX Drug Discovery Solutions for Metabolite Identification