MOVEMENT THROUGH THE MEMBRANE Bubble Lab Section 7-3

The cell membrane Function: Selective permeability Regulates what enters and leaves the cell. Provides protection and support Selective permeability Some substances can pass across the cell membrane and others can’t

Structure Phospholipid Bilayer (Fluid Mosaic Model): a double-layer sheet that makes up nearly all membranes.

Components of the lipid bilayer Polar Head Hydrophilic “Water Loving” Lipid Tails Hydrophobic “Water Fearing”

Why is the lipid bilayer important? Tough, flexible structure Strong barrier between the cell and its environment

What does it do for the cell? Allows only certain materials in or out at certain times. Ions Glucose Water Electrolytes

Other membrane structures Protein molecules Form channels and pumps that help to move material across the membrane

Other Membrane Structures Carbohydrate molecules Act like ID markers for the cell Basis for blood typing: the antigens on the blood cells (A, B, AB) are carbohydrate chains

Components of plasma membrane: 2 layers of phosphlipids Cells live in fluid environments, with water inside and outside the cell. Components of plasma membrane: 2 layers of phosphlipids Polar head and nonpolar tail Integral proteins Peripheral proteins Cholesterol Carbohydrates 11

Proteins: Peripheral proteins On inside surface Proteins: Peripheral proteins On inside surface Held in place by cytoskeletal filaments Integral proteins Embedded in membrane, can move laterally 12

Cholesterol - strengthens the plasma membrane. Cholesterol - strengthens the plasma membrane. Carbohydrates: Glycoproteins – proteins with carbohydrates attached Glycolipids – phospholipids with carbohydrates attached 13

Functions of membrane proteins Some help to transport materials across the membrane. Channel Protein – allows certain molecules or ions to cross membrane freely Carrier Protein – interacts with certain molecule or ions to help move it across membrane 14

Some proteins aid in cell recognition Glycocalyx - In animal cells, the carbohydrate chains of cell recognition proteins are collectively called this 15

Cell recognition protein Foreign carbohydrate chains are why transplanted tissue is often rejected by the body 16

Building A Membrane Using the materials provided to you, build a model of a cell membrane. Make a key explaining what pieces represent the Polar Head, Lipid Tails, Protein Molecules, and Carbohydrate Molecules Have your teacher check your model before you eat it!

Diffusion Definition: The movement of molecules from an area of higher concentration to an area of lower concentration, until equilibrium is reached. Concentration? Mass of Solute/Volume of solution (g/L) Diffusion of Molecules

More molecules = More collision What causes diffusion? Diffusion is caused by the constant movement and collision of molecules. More molecules = More collision The collision cause the molecules to spread out.

Diffusion in Cells Molecules are able to diffuse through the cell membrane and allow the cell to function. Facilitated Diffusion – some molecules need help from protein channels to cross the cell membrane. Animation

Osmosis (A special kind of diffusion) Osmosis is the diffusion of water molecules through a selectively permeable membrane. Selectively Permeable - Osmosis

Osmosis Isotonic – concentration is the same on both sides of the membrane (equilibrium) Hypertonic – the solution with a greater concentration Hypotonic – the solution with the lesser concentration.

Osmosis Practice The cytoplasm of Elodea cells is composed of about 70% water molecules and 30% other kinds of molecules. What happens when the Elodea cells are put into a liquid that is a. 50 percent water. b. 70 percent water. c. 100 percent water .

Active Transport Moving down a concentration gradient is like riding a bike down a hill. It doesn’t require energy Osmosis and diffusion are examples of passive transport! Moving up a concentration gradient is like riding up a hill. IT REQUIRES ENERGY.

Active Transport Active Transport: is the movement of materials through a cell membrane using energy. Molecules move from low concentration to high concentration. Proteins use ATP to pump ions and small molecules against concentration gradient.

Types of Active Transport Endocytosis – the process of taking material into the cell by means of infoldings of the cell membrane. Phagocytosis – extension of the cytoplasm surround and engulf the particle Pinocytosis – similar to phagocytosis, but cells take up liquid instead of particles.

Types of Active Transport 2. Exocytosis Large molecules move from inside the cell to outside the cell. Contractile Vacoule – an organelle that constantly pumps H2O out of the cell. Animation

Specialized Cells Cells with a specific structure and function and are found in multi-celled organisms. We have blood cells, brain cells, bone cells, liver cell, skin cells, etc.

We are all made of cells Organ System Organs Tissues Cells

Tissue Definition: A group of similar cells working together. Examples

Organs Definition: A groups of tissues working together to perform a specific job.

Organ Systems Definition: Many organs working together to do a specific job. Can you name all 10 organ systems?

7.3-7.4 Wrap up Questions Explain Diffusion Explain Osmosis What does selectively permeable mean? What is facilitated diffusion? Are phospholipids the only molecules in a cell membrane? What is active transport? What are tissues, organs, and organ systems? Give Examples

= cell 10% salt 10% salt 20% salt 10% salt 10% salt 20% salt = cell Label the environment around the cell in each beaker as hypertonic, hypotonic or isotonic. Label the net flow of water and state what will happen to the cell in each beaker? = cell 10% salt 10% salt 20% salt 10% salt 20% salt 10% salt Label the environment around the cell in each beaker as hypertonic, hypotonic or isotonic. Label the net flow of water and state what will happen to the cell in each beaker?

= cell 10% salt 10% salt 20% salt 10% salt 10% salt 20% salt Label the environment around the cell in each beaker as hypertonic, hypotonic or isotonic. Label the net flow of water and state what will happen to the cell in each beaker?

Cell membrane reading questions Compare and contrast integral and peripheral proteins. What are the differences between the two types of transport proteins discussed in the article (use figure 7.10 to help you) Why are proteins so essential to keeping your cells hydrated? Explain how diffusion relates to a concentration gradient.