Danielle Pogge
Chain of amino acids with a specific function Folding of protein determines function Enzymes, hormones, structural, etc Amino acids (20) Molecules containing an amine group Essential vs. non-essential Essential = required in the diet Lysine = 1 st limiting AA; Methionine Protein expressed as crude protein (CP) = 6.25 x % N Ex: DDGS, 29% CP = 4.6% N
Specific uses in the body: Maintenance = primary goal Growth Lactation Pregnancy (number of lambs) Wool Hormones and enzymes Antibodies
Ruminant Protein: N containing feeds, NPN, endogenous Ammonia for microbes Amino acids for animals (SI absorption) Dependent on: Stage of production Growth, gestation, lactation Level of production High vs. low producing Composition of gain Lean gain vs. fat Ewes = 9-15% (Highest = Lactation) Lambs = 11-20% (Highest = creep feeding) Decrease with age
Physical barriers Plant cell walls, cross linking of peptide chains Feed intake Rate of passage (ingestion to excretion = ~48 h) Fast = less degradation = more by-pass Rumen pH Predation of bacteria Protozoa = engulf bacteria Feed processing Heat damage (increase by-pass/decrease solubility) Maillard Reaction SBM, DDGS, Blood Meal Chemical damage (formaldehydes, tannins) Coating (lipid)
3 “Types” of protein: Degraded Intake Protein (DIP) Metabolized by microbes in rumen Microbial Crude Protein (MCP) Microbes themselves Bacteria = ~50% Protozoa = 20-60% Contribute ammonia from protein metabolism Undegraded Intake Protein (UIP) “By-pass protein” Absorbed in the small intestine Fate of proteins in a ruminant: Feed proteins Peptides Amino acids (rumen) Undegraded, escaped AA, “by-pass” proteins (SI)
Quality of protein is determined by the compatibility of AA profile to animals needs New NRC (Nutrient Requirement Council) Protein requirement based off % UIP More UIP = lower total protein requirement Ingredient% CP%UIP Corn7.540 Alfalfa Hay SBM4340 DDGS2762 Blood Meal Urea2870
Non-protein nitrogen (NPN) N not associated with protein Free AA, nucleic acids, amines, ammonia, nitrates, nitrites, urea Ex: Urea (287% CP) 100% degradable in the rumen Providing N for microbes Feeding NPN: If inadequate DIP available for microbes Precautions: Less than 1/3 of total protein intake Requires fermentable energy Increases the S requirement Church, 1988
DIP = 8-13% Total Digestible Nutrients (TDN) Below = benefit from NPN Over = excrete Ex: 100 lbs SBM = 49 lbs CP, 30 lbs DIP (49 lbs *.65 %DIP) 30 lbs DIP/87 lbs TDN = 34% of TDN % DIP% TDN% CP%BCP%DIP of TDN SBM Dry Corn * Alfalfa Hay Corn Stalks * DDGS Brome Pasture
Microbial sources of N: Diet protein NPN Recycled N Microbial synthesis: Dependent on ammonia and amino acids Energy Balance available ammonia for bacterial growth % of microbial protein entering SI: Low protein diet = ~60% High protein diet = ~40% NPN = 100% Church, 1988
Microbial protein yield = 0.13 lbs MCP/lb TDN MP yield = TDN x 0.13 Microbial protein: ~80% protein, with digestibility of ~80% in SI Nutritive value of microbial protein: Increases value of low quality feed Decreases value of high quality feed Can survive on NPN and low amounts of recycled N
Routes: saliva, across rumen wall, low rumen pH Dependent on protein in diet Low CP = increase recycling End of lactation dry diet = important! Intake Protein Protein Ammonia Urea NPN Microbial Protein Metabolizable Protein
Metabolizable protein = available for metabolism Absorbed amino acids Microbial protein (digestible) Undegraded protein (by-pass, digestible) Absorption Active transport: Intestines Portal blood Cells Na dependent Exchangers Amino acid availability for host: Quality, quantity, and degradation Undegraded protein and microbial protein
Church, 1988
High producing animals may need a greater percent of escape/bypass protein Ex. Ewes with triplets Lactation (maximize production) High quality alfalfa + corn for energy Nutrient use: Low quality forage (corn stalks/straw) Supplement protein High quality forage (alfalfa) Often meets requirements = rarely supplement protein
Management practices: Test hays for CP values Supplement on low quality forages (crop residue) Sort ewes by production level Lactation = greatest need (lose weight 2-6 weeks of lactation) Cheap protein sources: Soybean meal (46.5% CP) $310 = $0.33/lb CP DDGS (29% CP) $200 = $0.34 Alfalfa Hay (17% CP) $200 = $0.58
Protein intake MilkYieldMilkYield 1.28 TDN 1.86 TDN 2.35 TDN
Why we care: Protein deficiencies = reduces production Longer days on feed, decreased milk yield, etc. Depresses microbe function and nutrient digestion Protein excesses: EXPENSIVE! Increases animal’s maintenance requirement Environmental concerns Increased N excretion and run off Economics Question: Escape protein = expensive Is it worth it?
Protein requirements: Amino acids Microbial protein Energy is the limiting factor for protein utilization Protein deficiency = decreased production Milk, fertility, gain