PowerPoint Slides to Aid Learning with Forensic DNA Typing Book

Slides:

Advertisements

Similar presentations

Forensic Bioinformatic Services, Inc.

Advertisements

Brief History of Forensic DNA Typing

Forensic DNA Analysis (Part II)

Forensic DNA Typing or Did you kill (rape…) that person? How DNA can “definitively” say. Adapted from: National Institutes of Science & Technology

Chapter Eleven – Forensic DNA Analysis FRSC 8104 Criminalistics II Professor Bensley.

Crime Scene Investigator PCR Basics™

Forensic DNA Analysis Criminalist Harry Klann, DNA Technical Leader

DNA Analysis Chapter 12. General DNA Information Double helix—two coiled DNA strands Composed of nucleotides—units containing a sugar molecule (deoxyribose),

Explain how crime scene evidence is

DNA fingerprinting Every human carries a unique set of genes (except twins!) The order of the base pairs in the sequence of every human varies In a single.

Chapter 1. Speed of Analysis (Technology) Power of Discrimination (Genetics) Low High SlowFast Markers Used (Biology) RFLP Single Locus Probes RFLP Multi-Locus.

Forensic DNA Analysis Genome /04/2015. Forensics “Of, relating to, or denoting the application of scientific methods and techniques to the investigation.

Brief History of Forensic DNA Typing

DNA Forensics MUPGRET Workshop. “DNA evidence…offers prosecutors important new tools for the identification and apprehension of some of the most violent.

Fundamentals of Forensic DNA Typing

JS 111 DNA and Crime Welcome and Introductions Steven Lee- Instructor

DNA Fingerprinting Sotheavy Vann. What is DNA Fingerprinting?  “The generation of a set of distinct DNA fragments from a single DNA sample”  Aka DNA.

Forensics Chapter 8. Central Points  DNA testing can determine identity  DNA profiles are constructed in specialized laboratories  DNA profiles used.

Fundamentals of Forensic DNA Typing Slides prepared by John M. Butler June 2009 Chapter 7 DNA Amplification.

Chapter 17: Variable-Number Tandem Repeats Profiling.

explain how crime scene evidence is

1 Chapter 7 Chapter 7 DNA Fingerprinting Learning Goals: o Explain how crime scene evidence is collected and processed to obtain DNA o Describe how radioactive.

2 Historical Information w James Watson and Francis Crick discovered the configuration of the DNA molecule w Ray White describes first polymorphic.

Forensics Chapter 8. Central Points  DNA testing can determine identity  DNA profiles are constructed in specialized laboratories  DNA profiles used.

DNA in the Cell chromosome cell nucleus Double stranded DNA molecule Individual nucleotides PCR, stands for? Polymerase Chain Reaction.what is it? Invented.

Chapter 5 Biochemical Forensic Analysis: DNA Prof. J. T. Spencer Adjunct Prof. T. L. Meeks.

Forensic Sciences: DNA testing.  The application of a broad spectrum of sciences to answer questions of interest to the legal system.sciences.

Human Identity Testing Purpose: Match a person to a DNA sample. Examples: Paternity Test Genetic History Historical (Thomas Jefferson, Sally Hemings) Genealogical.

Chapter : DQA1/PM Chapter 18: Autosomal STR Profiling.

Forensic DNA Analysis (Part II). Summary What is DNA? Where is DNA found in the body? How does DNA differ among individuals? Forensic DNA Analysis DNA.

A Primer for Future Jurors (or Criminals)

DNA Analysis. Types of DNA Analysis Restriction Fragment Length Polymorphism (RFLP) examines Long repeats uses REs to cut DNA into thousands of fragments,

Crime Scene Investigator PCR Basics™

Progression of DNA Typing Markers

Gel Electrophoresis A molecular biology tool. Purpose To separate and analyze/compare fragments of DNA.

Forensic DNA: Use, Abuse, Promise, and Peril

Watson & Crick Discovered the basic shape of DNA

Figure 16.0 Watson and Crick. Figure 16.3 The structure of a DNA stand.

Forensic Science: Fundamentals & Investigations, Chapter 7 1 Introduction and History of Biological Evidence in Forensics DNA fingerprinting or DNA profiling,

Progression of DNA Typing Markers RFLP –multilocus VNTR probes –single locus VNTR probes ( 32 P and chemiluminescence) PCR –DQ-alpha (reverse dot blot)

Advantages of STR Analysis

Forensic DNA: Use, Abuse, Promise, and Peril William M. Shields.

Forensic DNA Analysis Basic Review 46 chromosomes per cell, 23 pairs Humans have approximately 25,000 genes Each gene has multiple versions,

Instructors Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education.

Crime Scene Investigator PCR Basics™

PCR Y.Martinez, LSHS, 2014 DIRECTIONS: COPY NOTES IN ORANGE.

Bio II: Forensics.  DNA molecules are found in the nucleus of cells in the human body in chromosomes.  People have 23 pairs of chromosomes, with an.

History Evidence BIOLOGICAL EVIDENCE EXAMINED FOR INHERITED TRAITS TECHNIQUES EMERGED FROM HEALTHCARE DNA FINGERPRINTING DEVELOPED IN 1984.

Chapter 8 Additional DNA Markers: Amelogenin, Y-Chromosome STRs, mtDNA, SNPs, Alu Repeats ©2002 Academic Press.

Polymerase Chain Reaction (PCR). What’s the point of PCR? PCR, or the polymerase chain reaction, makes copies of a specific piece of DNA PCR allows you.

 Types of STR markers- 5 types based on sequence  STR allele nomenclature  Allelic ladder  Serological methods of identity profiling  Identity profiling.

 ABO blood typing  Lacks power of discrimination  RFLP analysis using minisatellite probes  High power of discrimination  Laborious  STR analysis.

Three generations of DNA testing

Explain how crime scene evidence is

Explain how crime scene evidence is

explain how crime scene evidence is

History of Biological Evidence in Forensics

Explain how crime scene evidence is

Forensic Biology by Richard Li

Explain how crime scene evidence is

DNA Fingerprinting and Forensic Analysis

DNA Fingerprinting Chapter 7

DNA Analysis “The capacity to blunder slightly is the

Explain how crime scene evidence is

explain how crime scene evidence is

Structure and Function within Forensic Science

Explain how crime scene evidence is

Presentation transcript:

PowerPoint Slides to Aid Learning with Forensic DNA Typing Book Prepared by John M. Butler ©2002 Academic Press Forensic DNA Typing: The Biology and Technology Behind STR Markers

Forensic DNA Typing Book Cover FORENSIC DNA TYPING Biology and Technology behind STR Markers John M. Butler ©2002 Academic Press Published January 2001 Forensic DNA Typing Book Cover http://www.academicpress.com/apforensics

Overview and History of DNA Typing Chapter 1 Overview and History of DNA Typing ©2002 Academic Press

Montaret Davis Captured ©2002 Academic Press

Human Identity Testing Forensic cases -- matching suspect with evidence Paternity testing -- identifying father Historical investigations Missing persons investigations Mass disasters -- putting pieces back together Military DNA “dog tag” Convicted felon DNA databases ©2002 Academic Press

Progression of DNA Typing Markers RFLP multilocus VNTR probes single locus VNTR probes (32P and chemi) PCR DQ-alpha (reverse dot blot) PolyMarker (6 plex PCR; dots for SNPs) D1S80 (AMP-FLPs) singleplex STRs with silver staining multiplex STRs with fluorescent dyes ©2002 Academic Press

Comparison of DNA Typing Technologies Speed of Analysis (Technology) Power of Discrimination (Genetics) Low High Slow Fast Markers Used (Biology) RFLP Single Locus Probes Multi-Locus Probes ABO blood groups Multiplex STRs DQ single STR D1S80 mtDNA PolyMarker ©2002 Academic Press

Steps in Sample Processing Sample Obtained from Crime Scene or Paternity Investigation Biology DNA Extraction DNA Quantitation PCR Amplification of Multiple STR markers Technology Separation and Detection of PCR Products (STR Alleles) Sample Genotype Determination Genetics Comparison of Sample Genotype to Other Sample Results Generation of Case Report with Probability of Random Match If match occurs, comparison of DNA profile to population databases ©2002 Academic Press

Human Identity Testing Involves Comparing DNA Profiles gender ID A B C D E F G H I J DNA Size (base pairs) probability of a random match: ~1 in 3 trillion Results obtained in less than 5 hours with a spot of blood the size of a pinhead Simultaneous Analysis of 10 STRs and Gender ID ©2002 AP, Elsevier

Brief History of DNA Typing 1980 - Ray White describes first polymorphic RFLP marker 1985 - Alec Jeffreys discovers multilocus VNTR probes 1985 - first paper on PCR 1988 - FBI starts DNA casework 1991 - first STR paper 1995 - FSS starts UK DNA database 1998 - FBI launches CODIS database ©2002 Academic Press