Gyrase Mutation in Various Bacteria Leading to Fluoroquinolone (“Cipro”) Resistance By: Nancy Halliday Wesley Hanson Brenda Breeding.

Slides:

Advertisements

Similar presentations

Evolution and proteins You can see the effects of evolution, not only in the whole organism, but also in its molecules - DNA and protein For a mutation.

Advertisements

1 Introduction to Sequence Analysis Utah State University – Spring 2012 STAT 5570: Statistical Bioinformatics Notes 6.1.

Protein Targetting Prokaryotes vs. Eukaryotes Mutations

BIOINFORMATICS Ency Lee.

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc.

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc. 04.

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc

Integration of Bioinformatics into Inquiry Based Learning by Kathleen Gabric.

Biology Workbench Introduction. What is it used for? It is a web-browser to use bioinformatics tools to analyze and visualize nucleotide and protein sequences.

Protein structure Friday, 10 February 2006 Introduction to Bioinformatics Brigham Young University DA McClellan

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc

The construction of cells DNA or RNA Protein Carbohydrates Lipid etc.

Created by: Evelin Fuentes Rai Boone.  Genetic engineering : the deliberate manipulation of genetic material.  Genetic code : The sequence of nucleotides.

Arabidopsis Gene Project GK-12 April Workshop Karolyn Giang and Dr. Mulligan.

Express yourself That darn ribosome Mighty Mighty Proteins Mutants RNA to the Rescue

BIOLOGY CONCEPTS & CONNECTIONS Fourth Edition Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings Neil A. Campbell Jane B. Reece Lawrence.

Proteins (aka polypeptides)

Development of Bioinformatics and its application on Biotechnology

CHAPTER 10 Protein Synthesis

1 Going Green: Using GFP to Investigate Bioinformatics “It’s not easy being green!” Kermit the Frog Jon Connolly Susan Cronin Mary Elizabeth “M.E.” Jones.

RNA & Protein Synthesis Chapter 13. DNA A book of instructions that tells each individual what proteins to make for their needs. The path from genes to.

DNA & RNA Protein Synthesis. DNA be.com/watch?v =4PKjF7OumYo.

DNA Structure & Function. Perspective They knew where genes were (Morgan) They knew what chromosomes were made of Proteins & nucleic acids They didn’t.

Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.

Quiz (take out a sheet of paper 1. T/F DNA can exit the nucleus 2. Name the bases found in RNA 3. Name the enzyme used to construct RNA during transcription.

What are the parts of DNA? Vocabulary word for chapter 6.

Construction of Substitution Matrices

AP Biology DNA Study Guide. Chapter 16 Molecular Basis of Heredity The structure of DNA The major steps to replication The difference between replication,

ARE THESE ALL BEARS? WHICH ONES ARE MORE CLOSELY RELATED?

 Translation Creating Protein from mRNA Protein Structure  Proteins are made of Amino Acids.  There are 20 different Amino Acids.  The sequence of.

Supporting Scientific Collaboration Online SCOPE Workshop at San Diego Supercomputer Center March 19-22, 2008.

Amino Acid Structure From DNA a strand of mRNA is transcribed. This mRNA is read by a ribosome in the cytoplasm which in turn constructs the appropriate.

Bioinformatics Curriculum Issues, goals, curriculum.

Modeling tRNA’s Translator Function

Have your clickers ready!. 1. An amino acid. 2. A type of mutation 3. Three mRNA bases that code for an amino acid. 4. The genetic code. Countdown 30.

DNA to Protein. Chromosomes are made of tightly packed DNA A gene is a section of the DNA molecule that codes for a particular protein. The order of nitrogen.

Protein Synthesis The process of protein synthesis is explained by the central dogma of molecular biology, which states that: DNA  RNA  Proteins How.

DO NOW Please Read the Article on your desk Pick the sheets up on the cart Fill in the slot notes for HW – They are on my website.

 During replication (in DNA), an error may be made that causes changes in the mRNA and proteins made from that part of the DNA  These errors or changes.

Chapter 10 – Molecular Biology of the Gene $100 $200 $300 $400 $500 $100$100$100 $200 $300 $400 $500 Scientific Discoveries DNA and RNA Replication, Transcription.

What is a macromolecule? There are four main types of biological molecules called macromolecules. The four types of macromolecules are carbohydrates, lipids,

AP Biology Discussion Notes Thursday 2/18/2016. Goals for Today Be able to describe the possible affects and different types of mutations Be able to decode.

AP Biology Mutations Unit 5B.5. AP Biology Changes in genotype (DNA) can result in changes in phenotype  Alterations in DNA sequence can lead to changes.

Four Levels of Protein Structure Amino acids Primary structure.

Introducing Bioinformatics Using the Nitrogen Cycle Alyssa Bumbaugh Ron Peck Mark Radosevich.

Modern Genetics. What is DNA? James Watson and Francis Crick pieced together the structure of DNA in the 1950’s. DNA- Dexoyribo Nucleic Acid.

A change in the nucleotide sequence of DNA Ultimate source of genetic diversity Gene vs. Chromosome.

SC.912.L.16.3 DNA Replication. – During DNA replication, a double-stranded DNA molecule divides into two single strands. New nucleotides bond to each.

Bioinformatics Overview

RNA and protein synthesis

Variation among organisms

Gene Mutations.

3.11 Proteins are essential to the structures and activities of life

The Impact of Mutations

Protein Folding.

The Genetic Code Spring 2017.

Relationship between Genotype and Phenotype

Bioinformatics and BLAST

Antibiotic resistance

Relationship between Genotype and Phenotype

RNA and protein synthesis

CHAPTER 17 FROM GENE TO PROTEIN.

Have your clickers ready!

Friday March 27, 2015 Day 1 1. Please have these Items on your desk.

13.3 Mutations.

Presentation transcript:

Gyrase Mutation in Various Bacteria Leading to Fluoroquinolone (“Cipro”) Resistance By: Nancy Halliday Wesley Hanson Brenda Breeding

Audience: Undergraduate Science Students

Major Objectives Understand the relationships between DNA, RNA, and proteins. Explore the effects of tertiary protein structural changes on enzyme function. Learn to utilize tools available in bioinformatics.

Brief Overview : Students are presented journal articles discussing fluoroquinolone resistance in various bacteria as a result of Gyrase A mutation. Students seek to determine the caused of primary structure changes that lead to resistance. (Amino acid substitutions within the enzyme Gyrase A are explored.) Students search for thewww.ncbi.nlm.nih.gov appropriate protein code (1AB4) of the enzyme “gyrase”

Brief Overview(cont.): Students then utilize “Protein Explorer” to: 1. Visually examine the 3D structure of Gyrase A 2. Manipulate the program to identify the amino acid substitutions that are identified as the point of mutation(s). 3. Manipulate the program showing the structural change in the molecule once the mutation has taken place.

DNA Gyrase A and B Functional Dimer

Ser 83 Gyrase A Subunit

Asp 87 Ser 83 Gyrase A Subunit

Brief Overview(cont.): Students utilize “Biology Workbench” to: 1. Compare wildtype vs. mutated amino acid sequence. 2. Compare wildtype vs. mutated nucleic acid sequence. Sequences are available in the primary literature

Blank Insert slide of DNA/Amino Acid sequence alignments

Project Materials: Article: Rupp, J. et. al., Serine to asparagine substitution in the gyrase A gene leads to quinolone resistance in moxifloxacin-exposed Chlamydia pneumoniae. Website: Software: “Protein Explorerer” Software: “Biology Workbench”

Future Directions This initial exercise is used to introduce the problem space and tools available. Students are then assigned a specific antibiotic resistance model to explore mutations and protein tertiary structure changes that result from these mutations. Students then present results in a professional format.