GalliPro Bacillus based probiotic

Bacillus spore ideal as probiotic for chicken GalliPro Comprise live Bacillus spore – natural encapsulated bacteria Survive feed pelleting process and in feed storage Has compatibility with all EU approved coccidiostats Transform from spores to active cells in the intestine of chicken Reduce FCR Increase weight gain Increase nutrient utilization by excretion of enzymes Improves intestinal health and absorption capacity Modulate intestinal microflora resulting in more Lactic Acid Bacteria Reduce Salmonella and Campylobacter

Bacillus sp. Definition of Bacillus Spore formers Gram positive organisms Spore formers Bacillus is facultative aerobe but at present of nitrate/nitrite it can grow anaerobic Bacillus is found in soil and marine sediments Bacillus as probiotic is in the form of Bacillus spore – a dormant resistant stage that can transform to a vegetative cells Bacillus spores are pH stable at pH 2-3 Trypsin stable Thermo-tolerant In-Feed stable

Bacillus spores the ideal probiotic for chicken? Pelletizing study on selected probiotic strains on the market Pelletizing at 85C 30 sec.: External facility, Bioteknologisk Institut, Denmark Microbiological analysis: External lab, LUFA –ITL-GmbH, Germany Result: Viable cell count in average CFU/g feed and the recover of viable Sample ID Calculated CFU/g Meal feed Pellet feed Recover of cells after pelleting, % Control total aerobic NA 2.6E+06 3.6E+04 1 L.farcimis 3.1E+04 < 10 <1 P.acidilactici 4.0E+06 3.6E+06 2.7E+04 E.faecium 8.5E+06 1.5E+06 18 S.cereviciae 8.0E+06 B.licheniformis 1.3E+06 1.2E+06 100 GalliPro

Bacillus spores the ideal probiotic for chicken? Bacillus spore extremely resistant – easy to apply into feed Bacillus spore germination occurred rapidly in the GI tracts of chicken (Cartman et al 2008) Vegetative cells can be detected throughout the GI tract of chicks (Cartman et al 2008) Feeding Bacillus subtilis to chicken resulted in greater growth performance and intestinal histologies such as increasing villus height. Villus tip showed no damage (Samanya & Yamauchi 2002) Indicating improved nutrient utilization capacity

Bacillus spores the ideal probiotic for chicken? Feeding GalliPro More diverse and complex bacteria composition in the gut Expectedly provides more robust microbiota less susceptible towards diseases and infections (Lan et al 2005) High numbers of Lactic Acid Bacteria Considered beneficial due to their putative health properties – protect against pathogens colonization and immune stimulation of the host Lactobacillus salivarius Strong probiotic characteristics reducing Salmonella and E. coli (Pascual et al 1999, Brashears et al 2003) Lactobacillus paracasei Stimulate the immune system of the host (Herich et al 2002, Morelli et al 2003)

GalliPro reduces the level of Campylobacter in broiler chickens The result shows a significant reduction of Campylobacter by 52% in the GalliPro treated group. The results on ileal samples showed a 40% reduction of Campylobacter. Our finding of Campylobacter is accordance with literature. Corry and Atabay (2001) reported the commonly observed level in bird intestinal to be 105 to 107 CFU/g.

GalliPro significantly reduce Salmonella in chicken Salmonella challenge pen floor study (SPR, US) with and without GalliPro showed a significantly reduction of Salmonella in the GalliPro treated group All samples were found positive at day 42 in the control group whereas only 43% of the samples in the GalliPro group were positive with regards to Salmonella a b Different letters indication significant difference P< 0.05

Bacillus spores the ideal probiotic for chicken? GalliPro produce enzymes Alcalin phosphatase Esterase Esterase lipase Lipase Leucine amino-peptidase Valine amino–peptidase Acid phosphatase Glucosidase Bacillus shows amylolitic (starch), lipolytic (fat) and proteolytic (protein) activities

Summary of 17 trials GalliPro vs. Negative Control Relative efficacy of GalliPro in broiler trials at independent research institutes Avg. improvement: Final bodyweight +2,8% & FCR -3,8% +2,8% -3,8%

Big increases in in-vivo nutrient utilisation ME 100% diet Control GalliPro Diff Dry matter ileal digestibility (%) 74,1 78,0 3,9 Crude protein ileal digestibility (%) 82,6 85,6 3,0 Gross energy ileal digestibility (%) 77,0 81,0 4,0 N retention (%) 66,6 68,8 2,2 ME (MJ/kg DM) 13,88 14,19 0,3 ME (MJ/kg DM, N corrected) 13,46 13,76 GalliPro increased in-vivo dry matter, crude protein and gross energy digestibilities, N retention and metabolisable energy. Study 2 at University Federal de Viçosa, Brazil

GalliPro Works! Increased in-vivo DM, CP & GE digestibilities Increased weight gain, improved FCR and lower mortality Canadian & EU Registration Safe, stable and easy to use Consistent performance

Bacillus spores the ideal probiotic for chicken? Bacillus subtilis (DSM17299) compatibility with Coccidiostats: Amprolium/ethopabate Diclazuril Halofuginone Lasalocid sodium Maduramicin ammonium Methylbenzoquate/meticlorpindol Monensin sodium Narasin Nicarbazin Nifursol Robenidine Salinomycin sodium Semduramicin