Genetic Engineering Biotechnology Molecular Cloning Recombinant DNA

HISTORY Stanley Falkow Stanley Cohen Herb Boyer Competent Cells EcoR1 Plasmids Herb Boyer EcoR1 Stanley Falkow

Asilomar Conference 1975 Voluntary Band of Some Recombinant Research NIH-RAC formed as a result

Isolate Gene Fragmented Total DNA Use mRNA and Make DNA with Reverse Transcriptase

Splice into Cloning Vehicle Plasmids Viruses Use Restriction Endonuclease to create – Sticking Ends Rejoin Molecule with Ligase

Inserting into Host Transformation Detection of Successful Clone DNA Library Shotgun Cloning

Cloning Vector Plasmid Cloning Vectors Single Cleavage Site High copy Numbers Insertion Inactivation

Virus Vectors Lambda Phage Vector Stable Packaging Cosmids

Other Vectors Expression Vectors Secretion Vectors Shuttle Vectors Bacteriophage M13 DNA Sequencing Yeast Artificial Chromosome Human Genome Project

Hosts Cloning Vector Hosts E. coli Endotoxins Yeast

Detection of Clones Antibodies Nucleic Acid Probes Synthetic DNA

Production of Recombinant Protein Transcription of Cloned Gene Promoter Translation of Cloned Gene Ribosome Binding Site

Polymerase Chain Reaction Kary Mullins PCR DNA Amplification TAQ Enzyme Primer

PCR

Site Directed Mutagenesis Knockout Mutation

Applications of Genetic Engineering Human Insulin Interferon Hepatitis Vaccine

Vaccines Subunit Vaccines Hepatitis Vaccine Recombinant Vaccines Polyvalent Vaccines DNA Vaccines Edible Vaccines

Other Products Tissue Plasminogen Activator Rennin Ti Plasmid Bacillus thuringiensis – BT Roundup Resistant Favosavr Tomato

More Possibilities Ice Minus Bacteria PCB Degrading Bacteria Luminescent Reporter Gene Transgenic Animal Human Gene Therapy

DNA Chips - Microarrays Actual DNA Chip

Genetic Eng. Word List Splice into Cloning Vehicle - Biotechnology Plasmids, Viruses Restriction Endonucleases Sticky Ends Ligase Insert into Host Biotechnology Molecular Cloning Recombinant DNA Isolate Gene mRNA, Reverse Transcriptase Detect Successful Clone DNA Library Shotgun Cloning Plasmid Cloning Vectors Single Cleavage Sites

Genetic Eng. Word List 2 Other Vectors Human Genome Project Expression Vectors Secretion Vectors Shuttle Vectors Bacteriophage M 13 DNA Sequencing Yeast Artificial Chromosomes Human Genome Project Cloning Vector Hosts E. coli Yeast Detection of Clones Antibody High Copy numbers Insertion Inactivation Lambda Phage Vector Stable Packaging Cosmids

Genetic Eng. Word List 3 Nucleic Acid Probes Transcription of Cloned Gene Promoter Translation of Cloned Gene Ribosome Binding Site Synthetic DNA Probes Polymerase chain Reaction – PCR DNA Amplification TAQ Enzyme Primers Site Directed Mutagenesis Knockout Mutations Applications of Genetic Engineering Human Insulin Interferon Hepatitis Vaccine Recombinant Vaccine Subunit Vaccines

Genetic Eng. Word List 4 Polyvalent Vaccines DNA Vaccines Tissue Plasminogen Activator Rennin Ti Plasmid Bacillus thuringiensis Edible Vaccines Human Gene Therapy DNA Chips – Microarrays Roundup Resistance Favosavr Tomato Ice Minus Bacteria PCB Degrading Bacteria Luminescent Reporter Gene Transgenic Animals