Folic Acid A member of vitamin B-complex. Slightly solubility in water. Odorless. Antianemic, “food addictive “ and reproduction optimizer. C 19 H 19 N 7 O 6

- The hydrosoluble form of folic acid. - Optimizes the enzymatic metabolism of bacteria. - Reduces the generated amount of sludge. - Activates the ammonia nitrification. - Ends the excessive growth of filamentous bacteria. - Since no similar products. - Developed for 20 years. Dosfolat (Tetrahydrofolicacid)

- Density: 1.25 g/cm 3 - pH ( ppm): Shock dosage: 0,5 to 0,7 ppm, more often 0,5. - Operation dosage, accordingly to COD:  up to 2000 mg/l  0,1 ppm  between 5000 and 5500  0,2 ppm  above 5500  0,3 ppm - Increase of stalk protozoa, decrease of turbidity. - Contains nitrogen, promoting the decrease of urea dosage.

- Dosage:  Dilution 1:100 (initial), 1:200 or 1:400 (industrial effluents, high load or organic toxics), in distilled or demineralized water.  Solution valid for 1 week, maximum tolerance 14 days.  Water quality testing for solution (in case of light water): prepare a 1% solution, let it rest for 24 hours, if no precipitation or turbidity is observed, water is apt. - Dosage point: TQ-AR entrance (next to recycling).

Cloudiness means poor quality dilution water

Phases of action of Dosfolat General summary 1. Increase of active microorganisms population 2. More efficient oxidation, requiring less contact time OM / bacteria 3. Thus, we are able to raise the sludge age 4. As oxidation is faster, there is no sufficient time for solids (biomass) increase 5. Working with regular levels of solids in the system, high degradability and high sludge age (IL), sludge discharge is reduced.

Experience 1 Santista Têxtil (Americana - SP) - Beginning of tests in Jan/98. Plant characteristics: - Main objectives: Increase microbiota population (observation of flocs) Increase efficiency in BOD/COD removal Reduce number of filamentous bacteria Decrease generation of sludge (raise IL level to 30 days) Decrease in color Flowrate (Q): 95 m 3 /h BOD2: 1100 mg/l COD2: 1800 mg/l (Ef removal: 85%) IL: 20 days SST3: 3400 ml/l Sludge Generation: 1100 kg/day (dry base)

- Operation: Initial dosage, or shock dosage, with duration of 15 days  0,5 mg/l (19,26 kg) Operational Dosage  0,2 mg/l (15,40 kg/month) Solution 1:400, with demineralized water Periodical increasing on the sludge age

Dosing pump Qr Q TQ-AR 6400 m 3 Scheme for preparation tank and dosage point Outlet to DC 1000 liters polyethylene tank, with lid

Main Results Microbiology: decrease of filaments, improving the settleability, reducing SVI and presenting larger and more cohese flocs. Proliferation of stalked ciliate protozoa was observed, indicating stability of the plant. Color: the ETS had a history of 2410 mg/l of PtCo, we reached 574 with stabilization at 1409 mg/l of PtCo. SOUR (specific oxygen uptake rate): used to maintain a mean value of 10 mgO2/h/g of SSV. By the end of the essays the ETS was already working with a SOUR of 165 mgO2/h/g of SSV. COD removal: increase of efficiency, from 85 % to 93 % Decrease of the generated amount of sludge: compared to 5 days prior to the beginning of administration: 23 % compared to the last 30 days prior to the beginning of administration: 38,7 % compared to the last 90 days: 38 % compared to the last 12 months: 36 %

Results 1.Increase of efficiency in COD removal B. Decrease of final values 20 days25 days30 days

Results 2. Reduction of Color 20 days25 days30 days

Results 3. Reduction of SVI 20 days25 days30 days

Results 4. Reduction of generated sludge 20 days25 days30 days

Microbiology

Before Application

After Application

Experience 2 Fibralin Têxtil (Itatiba - SP) - Beginning of tests in July/98, plant characteristics: - Main Objectives: Increase microbiota population (observation of flocs) Increase efficiency in BOD/COD removal Reduce number of filamentous bacteria Decrease generation of sludge (raise IL level to 20 days) Decrease in color Flowrate (Q): 15 m 3 /h BOD2: 1800 mg/l COD2: 3000 mg/l (Ef of 85 %) IL: 14 days SST3: 3100 ml/l Sludge Generation: 675 kg/day (dry basis)

- Operation: Shock dosage, with duration of 15 days  0,5 mg/l (0,24 kg/day) Operational dosage  0,3 mg/l (0,144 kg/d) Solution 1:400, with demineralized water Periodical increasing on the age of sludge

Sludge Reduction After 2 years, we have observed the following pattern of reduction: 3 first months: 46 % 6 months: 70 % 1 year and 5 months: 85 % 2 years: 99,6 %

Experience 3 Vine Têxtil S/A - F I - (Itatiba - SP) Discharge closed in 35 days, sustained till today (18 months)

IMPORTANT OBSERVATIONS Never interrupt dosage: breakage of degradation cycle Use only demineralized or distilled water: avoid clots Avoid direct action of UV rays Basic rules of manipulation Don’t use metal barrels for storage of solution