Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

Slides:



Advertisements
Similar presentations
The identification proteins using mass spectrometry
Advertisements

Separation of proteins in the 1 st dimension Dr Kevin Mills Institute of Child Health, UCL, London.
From Genome to Proteome Juang RH (2004) BCbasics Systems Biology, Integrated Biology.
Using Skyline to Monitor Long- Term Performance Metrics of High-Resolution Mass Spectrometers J. Will Thompson and M. Arthur Moseley Duke Proteomics Core.
The Proteomics Core at Wayne State University
Immunity & Infection Research Centre Better Biomarkers in Transplantation. A Genome Canada Initiative for Human Health Rob McMaster, Gabriela Cohen-Freue,
MS of Intact Complexes and Hybrid Technologies
Proteored Multicentric Experiment 8 (PME8) Quantitative Targeted Analysis in Proteomics. An Assesment Study (QTAPAS) ProteoRed WG1-WG2 Meeting Pamplona,
URIMEM, will facilitate biomarker discovery and change the face of medicine 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical.
Mass Spectrometry at The University of Louisville HSC
The Golden Age of Biology DNA -> RNA -> Proteins -> Metabolites Genomics Technologies MECHANISMS OF LIFE Health Care Diagnostics Medicines Animal Products.
Technische Universität München Cancer biomarker discovery by chemical and quantitative proteomics Sebastian Berger, B. Sc., Center of Life and.
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Lipidomics.
HOW MASS SPECTROMETRY CAN IMPROVE YOUR RESEARCH
My contact details and information about submitting samples for MS
Proteomics Informatics Workshop Part III: Protein Quantitation
Proteomics Informatics Workshop Part II: Protein Characterization David Fenyö February 18, 2011 Top-down/bottom-up proteomics Post-translational modifications.
Evaluated Reference MS/MS Spectra Libraries Current and Future NIST Programs.
Isolation of N-linked glycopeptides from plasma Yong Zhou 1, Ruedi Aebersold 2, and Hui Zhang 1,3 * 1 Institute for Systems Biology, Seattle, Washington.
1 Quantitative Proteomic Sample Set Amounts displayed as fmols per µg of digested yeast lysate Each sample theoretically contains 15 µg of digested yeast.
HPP Preliminary Results La Cristalera, August 2012 Montserrat Carrascal, Joan Villanueva, Joaquín Abián LP-CSIC/UAB.
© 2010 SRI International - Company Confidential and Proprietary Information Quantitative Proteomics: Approaches and Current Capabilities Pathway Tools.
GSAT501 - proteomics Name, home-town Students – previous lab experience –Lab you hope to end up in? Teachers – what is your current project.
Bringing Metrology to Clinical Proteomic Research David Bunk Chemical Science and Technology Laboratory National Institute of Standards and Technology.
Proteomics What is it? How is it done? Are there different kinds? Why would you want to do it (what can it tell you)?
June 9th, 2013 Matthew J. Rardin June 9th, 2013 Matthew J. Rardin MS1 and MS2 crosstalk in label free quantitation of mass spectrometry data independent.
Host Response to HIV-1 Infection: Quantitative Proteomics & Allied Approaches Eric Chan.
Set Sail Worktext p. 133.
Proteomics Informatics (BMSC-GA 4437) Instructor David Fenyö Contact information
Salamanca, March 16th 2010 Participants: Laboratori de Proteomica-HUVH Servicio de Proteómica-CNB-CSIC Participants: Laboratori de Proteomica-HUVH Servicio.
Oct 2011 SDMBT1 Lecture 11 Some quantitation methods with LC-MS a.ICAT b.iTRAQ c.Proteolytic 18 O labelling d.SILAC e.AQUA f.Label Free quantitation.
LO: to round to the nearest 10 Which ten is the following number nearest to?
Protein quantitation I: Overview (Week 5). Fractionation Digestion LC-MS Lysis MS Sample i Protein j Peptide k Proteomic Bioinformatics – Quantitation.
Ho-Tak Lau, Hyong Won Suh, Martin Golkowski, and Shao-En Ong
Custom peptide synthesis services In the quantitative proteomics research, several MS-based methodologies for relative quantification have been introduced.
Custom peptide synthesis services In the quantitative proteomics research, several MS-based methodologies for relative quantification have been introduced.
Post translational modification n- acetylation Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying unknown protein. Proteins to.
Mass spectrometry data enhancement software
36 LO: to round to the nearest 10
Skyline not identifying peptides
4,135,652 Place Value Hundred Thousands Ten Thousands Hundreds
Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Metabolomics.
Objective - To round whole numbers.
2 Dimensional Gel Electrophoresis
The Children’s Heart Study
L l l l l l l l l l l 783.
Place Value.
Comparing Numbers.
Place Value © Ashley Lee, 2013.
Relationship of Numbers in the Place Value System
Thomas BOTZANOWSKI & Blandine CHAZARIN
Quantitative proteomics reveals daf‐16‐mediated reduction in protein metabolism in long‐lived daf‐2(e1370) mutants. Quantitative proteomics reveals daf‐16‐mediated.
4,135,652 Place Value Hundred Thousands Ten Thousands Hundreds
Getting confused when visualizing numbers that are bigger than 1,000. For example: How do you use base ten blocks to represent 100,000?
Expanded Notation , , , , , , Name: _________________________________
Volume 65, Issue 2, Pages (January 2017)
Proteomic Approaches to Cancer Biomarkers
A quantitative proteomics strategy to identify SUMO-conjugated proteins. A quantitative proteomics strategy to identify SUMO-conjugated proteins. HeLa.
Total ion current profiles across the chromatographic peptide separation for digests of undepleted (A, whole) and depleted (B) plasma from experiments.
Place Value.
Objective - To read, write and identify the values of whole numbers.
A, Base peak chromatogram of apomyoglobin digest generated by 0
Is Proteomics the New Genomics?
Shotgun Proteomics in Neuroscience
Chromatograms, MS and MS/MS spectra obtained by LC-MS/MS (Q-TOF) of peptides from UPIII identified after Western blotting followed by on-membrane digestion.
Protein identification using MS/MS.
Acetylation is a dynamic modification in B. subtilis.
Comparing Numbers.
Presentation transcript:

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

Label-free quantitative proteomics using qTOF MS Sample, Tissue, Plasma Yeast Enolase (100 fmol) lyse Individual proteins

lyse Thousands of proteins Trypsin digest all proteins to peptides Tens/ hundreds of thousands of peptides QTOF Sequence everything Sample Label-free quantitative proteomics using qTOF MS

Time Amount of peptide (larger the response, more protein present)

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

UCL Institute of Child Health & Great Ormond Street Hospital for Sick Children UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Acknowledgements UCL

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2024 SlidePlayer.com Inc. All rights reserved.