Genomes To Life User Facilities for 21 st Century Systems Biology A Joint Initiative of the Office of Advanced Scientific Computing Research and the Office of Biological and Environmental Research Dr. Marvin Frazier December 3, 2002 Biological and Environmental Research Advisory Committee Meeting

Growing Mandate to Move Biology Forward “Molecular Level Understanding of Life Processes” is a National Science Priority. — OMB/OSTP FY 2004 Budget Guidance Memo “Instrument development & new facilities are critical needs in microbial science.” — American Academy of Microbiology 2001 Colloquium, “Microbial Ecology and Genomics: A Crossroads of Opportunity” “DOE should now create unique, high throughput research facilities and resources to translate the new biology, embodied in the Genomes to Life (GTL) program, into a reality for the nation.” —Biological and Environmental Research Advisory Committee Agency Comments R. Aebersold and J. D. Watts, “The Need for National Centers for Proteomics,” Nature Biotechnology, 20: 651 (July 2002).

GTL User Facilities - Introduction Planning Update Description of facilities Path Forward Dr. Abigail Salyers, Past President, American Society of Microbiology

GTL User Facilities Planning Update BERAC Report – April San Francisco Workshop – June Dr. Orbach’s Call for Office of Science - August Chicago Workshop – August Gaithersburg Workshop – October Report to Orbach – November BERAC Presentations - December

Workshop Participants Eric Ackerman, PNNL Kritsin Balder-Froid, LBNL Jim Bixler, PNNL Jim Brainard, LANL Elbert Branscomb, LLNL Michelle Buchanan, ORNL Steve Colson, PNNL Mike Colvin, LLNL Brian Davison, ORNL Graham Fleming, LBNL Jim Fredrickson, PNNL Paul Gilna, LANL Gary Jacobs, ORNL Michael Kennedy, PNNL Michael Knotek, Consultant Lee Makowski, ANL Betty Mansfield, ORNL Bob Marianelli, Consultant Ken Nealson, USC Rick Stevens, ANL Jill Trewhella, LANL Ed Uberbacher, ORNL DOE Participants Dan Drell Brendlyn Faison Marvin Frazier Teresa Fryberger John Houghton Peter Kirchner Noelle Metting Ari Patrinos Mike Riches Michael Teresinski David Thomassen Mike Viola

Genomes to Life: User Facilities for 21 st Century Systems Biology Biotechnology Revolution offers DOE mission solutions The genome is the key to major advancements in understanding and applications Microbes are Us: Major Discoveries and Diverse Beneficial Capabilities The Genomic Approach: High-Tech, High-Throughput, and High-Quality Data to free the scientist to do science Pilot Projects and Technology Development: We have already established the bases for facilities We have a plan: Science, Technologies, Facilities, and Management

DOE R&D Contributed to: Decreased Cost Increased Throughput Providing the Paradigm for Genomes to Life Facilities Human Genome Project Making the Impossible Routine Cost per base in dollars. Millions of bases finished.

Facility cost estimates are based on New Construction and will meet the elimination of excess facility requirements. Cost estimates are for a fully equipped and completely functional facility. Upon approval of the concept other external funding sources (e.g., third party, multiple agency, etc.) will be considered as appropriate. Facility Cost Estimates=$885M

Management Principles for Genomes to Life User Facilities An open-acquisition process that encourages broad involvement of the national laboratories, academia, and industry in innovation and technology development, facility design and development, as well as host site selection Peer-review process for determining the target and products of the facilities An open availability of facilities capabilities and free dissemination of data, tools, and materials

GTL Facilities Hallmarks Open, Rapid User Access to Data and Facilities Advanced Technologies Microbial Genomics High- Throughput Analysis and Production Biosystems Training Informatics and Databases Computing and Simulation Technology Integration for Tomorrow’s Biology