National Centre for Biotechnology Education www.ncbe.reading.ac.uk Nature’s dice Copyright © Dean Madden, 2010.

Slides:

Advertisements

Similar presentations

Carry out PCR for 20, 25 and 30 cycles. Analyse the PCR fragments by agarose gel electrophoresis. Find out how the number of cycles affects the amount.

Advertisements

National Centre for Biotechnology Education The Lambda protocol Copyright © Dean Madden, 2012.

Loading Gels. The Gel Box Electrode Lid Tray to hold gel Connect to power supply.

An Introduction to Microvolumetrics and Pipetting

Gel Electrophoresis Lab Analysis of Precut Lambda DNA.

Science and Plants for Schools, SSERC DNA Profiling and Biodiversity Kath Crawford.

Agarose gel electrophoresis

Gel Electrophoresis Gel Electrophoresis.

Preparing Agarose Gels

DNA-Fingerprint1 Procedure of DNA-Fingerprints. DNA-Fingerprint2 Tubes for each workgroup.

BRIDGES 2014 Agarose Gel Visualization of Restriction Enzyme Digest.

Plasmid preparation and Restriction digestion

A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes.

A Timed Presentation - do not click the mouse. Approximate Run Time – 4 minutes and 45 seconds.

General Genetics.  To learn how to prepare agarose gel electrophoresis.

Prepered by:- Rana Al-Turki

A Timed Presentation - do not click the mouse. Approximate Run Time - 4 minutes 5 seconds.

DNA-Fingerprint1 Detection of PCR Products by Agarose Gel Electrophoresis.

Practical #2: Extraction of genomic DNA from E.Coli Practical #3: Agarose Gel Electrophoresis Bertrand Ong Chan JianPeng Salanne Lee.

National Centre for Biotechnology Education The PCR and Plant evolution Copyright © Dean Madden, 2012.

National Centre for Biotechnology Education Gel electrophoresis Protein power! Copyright © Dean Madden, 2010.

National Centre for Biotechnology Education and Science and Plants for Schools Investigating Photosynthesis Copyright © Dean Madden,

This procedure depends on : the nucleic acid charge and the used solution polarity. DNA molecules are negative (-) in charge. When DNA exposed to electrical.

Collect Buccal Cells. PCR Polymerase Chain Reaction DNA/gene amplification.

DNA Isolation, Restriction Enzyme, Digestion, and Gel Electrophoresis.

Gel Electrophoresis Lab

Electrophoresis Electrophoresis is the movement of molecules by an electric current .This is practically done in a matrix to limit migration and contain.

DNA Technology: BACTERIAL TRANSFORMATION Ms. Gaynor Honors Genetics.

1962: Nobel Prize in Physiology and Medicine James D. Watson Francis H. Crick Maurice H. F. Wilkins What about? Rosalind Franklin.

KEYS Lab Training DNA Barcoding: Identification of Species

AP Biology: Lab 6. In the early 1970s scientists discovered the genetic code is universal - the same for all living things. This has enabled scientists.

Unit 8 Vocabulary 1. micropipette- tool used to transfer small volumes of liquid 2. P 20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used.

Restriction Analysis and Digestion of Lambda DNA.

Chapter 3: Units of Measurement The most important basic units for biologists are : meters (m) for length grams (g) for mass liters (L) for volume.

Biotechnology Introduction Gel Electrophoresis. Method of analyzing DNA Allows a researcher to determine how alike or different two samples of DNA are.

GEL ELECTROPHORESIS. FIRST, WHAT……  Simply put, gel electrophoresis is a technique used to separate molecules such a DNA, RNA and proteins according.

Gel Electrophoresis. Definition – COPY ME! Separation of DNA fragments according to size and charge Based on movement through a gel medium when an.

Restriction Digestion and Gel Electrophoresis Laboratory.

Gel Electrophoresis.

DNA Restriction Analysis. 1. Obtain 4 tubes Use permanent marker to label B, E, H, C (on the lids) B = BamH1 E = EcoR1 H = Hind111 C= Control.

1. What part of the DNA molecule results in its slightly negative charge? a.The pentose deoxyribose sugar b.The Adenine nucleotide c.The phosphate group.

A Timed Presentation - do not click the mouse. Approximate Run Time - 2 minutes 38 seconds.

DNA Fingerprinting Agarose Gel Electrophoresis Student Instructions

National Centre for Biotechnology Education The Microbial fuel cell Copyright © Dean Madden, 2015.

Lab.3 Gel electrophoresis

LAB 6 DNA FINGERPRINTING. BUILD the GEL FRAME Position comb and lock in place in side slots.

A 2 nd year college student working in the lab for the summer forgot to label the Tube of plasmid DNA that he/she was given. So being the brilliant graduate.

DNA fingerprinting made simple Cat no 130 Edvotek Europe.

Agarose Gel Electrophoresis Gel electrophoresis is a widely used technique for the analysis of nucleic acids and proteins. Agarose gel electrophoresis.

Restriction Enzyme Digestion of Phage DNA

DNA Paternity Test RFLP analysis

An Introduction to Microvolumetrics and Pipetting

Gel Electrophoresis of DNA

Gel Electrophoresis By: Sariah Arnold.

Agarose gel electrophoresis

Copyright © Dean Madden, 2010

Introduction to Gel Electrophoresis

Opening Activity: March 12, 2018

An Introduction to Microvolumetrics and Pipetting

DNA Technology: GEL ELECTROHPHORESIS

Agarose gel Electrophoresis

Electrophoresis… an analysis tool.

DNA Technology: GEL ELECTROHPHORESIS

Gel Electrophoresis.

Agarose Gel Electrophoresis

Discover the Microbes Within

Opening Activity: March 20, 2017

Gel Electrophoresis: Introduction and Techniques

Forensic DNA Fingerprinting:

Presentation transcript:

National Centre for Biotechnology Education Nature’s dice Copyright © Dean Madden, 2010

Sickle cell Normal red blood cells

Copyright © Dean Madden, 2010

Dominant allele (D) Recessive allele (d) DNA remains uncut DNA is cut Restriction enzyme 6500 base-pairs (bp) 2500 bp4000 bp

Copyright © Dean Madden, 2010

Summary of procedure DNA Enzyme Loading dye

Copyright © Dean Madden, 2010 Microsyringe Graduated tip HOLD HERE Do not touch the point! 10 µL 2 µL

Copyright © Dean Madden, 2010 Mass A microgram is one millionth of a gram micrograms (µg) = 1 milligram (mg) milligrams = 1 gram (g) Volume A microlitre is one millionth of a litre microlitres (µL) = 1 millilitre (mL) millilitres = 1 litre (L)

Copyright © Dean Madden, 2010 IMPORTANT Mix well after dispensing 20 µL Numbered DNA sample Dried restriction enzyme in tube Label the tube twice (on the side and on the lid)

Copyright © Dean Madden, 2010 Close the tubes and push them firmly into the foam floater Incubate for 30–45 minutes at 37°C

Copyright © Dean Madden, 2010 Frosted panel on this side Molten agarose 55–60 °C

Copyright © Dean Madden, 2010 Cut two electrodes Carbon fibre tissue 42 mm 22 mm

Copyright © Dean Madden, 2010 Pour 2–3 mm depth of buffer over the gel before you ease the comb out

Copyright © Dean Madden, 2010 Mix loading dye into each DNA sample 2 µL Bromophenol blue loading dye Cut DNA sample

Copyright © Dean Madden, 2010 Label the end of the tank to show the contents of each well Black card under the tank reveals the wells for loading Load the DNA through the buffer, taking care not to puncture the wells as you do so

Copyright © Dean Madden, 2010 Electrodes

Copyright © Dean Madden, 2010 Direction of DNA movement Place a comb over the tank to reduce evaporation

Copyright © Dean Madden, 2010 Leave the stain on for 4 minutes only Azure A stain

Copyright © Dean Madden, 2010 DNA Positively-charged Azure A binds to the negatively-charged phosphate groups of the DNA

Copyright © Dean Madden, 2010 Area with DNA bands Wells Loading dye Compare each band from each DNA sample with bands of known sizes in the DNA ‘ruler’

Copyright © Dean Madden, 2010 DNA ‘ruler’ or ‘ladder’

Copyright © Dean Madden, 2010 UnaffectedAffectedCarrier