DNA Laboratory SUNY College at Oneonta Dr. Nancy Bachman.

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Presentation transcript:

DNA Laboratory SUNY College at Oneonta Dr. Nancy Bachman

Cells The ultimate microscale computers The ultimate microscale computers Different parts have special functions Different parts have special functions –Nucleus:heredity –Mitochondria:energy

DNA: Deoxyribonucleic Acid Found in cell nucleus Found in cell nucleus Provides the blueprint for proteins Provides the blueprint for proteins Order of bases specifies the code Order of bases specifies the code

Nucleotides Subunits of DNA Subunits of DNA = Sugar +phosphate +base = Sugar +phosphate +base Bases are Bases are A, C, G, T A, C, G, T

Laboratory methods DNA purification DNA purification PCR (polymerase chain reaction) PCR (polymerase chain reaction) Gel electrophoresis Gel electrophoresis DNA sequencing DNA sequencing

Applications Forensics Forensics Genetic Diseases Genetic Diseases Cell/Molecular Biology Research Cell/Molecular Biology Research Biocomputing Biocomputing

DNA isolation

PCR

DNA sequencing

Luminescence

Fluorescence

Fluorescent microscopy

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