Effect of Certain Agonists & Their Antagonists on The Isolated Rabbit Intestine Lab #3.

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Effect of Certain Agonists & Their Antagonists on The Isolated Rabbit Intestine Lab #3

Stimulants & their Blockers 1-Drugs act on Nn R Agonist Dil. Nicotine (0.01%, 0.1ml) MOA: Dil. Nicotine stimulates Gs protein in cell membrane → activates Na-K channels → ↑ intracellular Na → rapid depolarization → contraction Antagonist Conc. Nicotine (0.1%, 0.1ml) Conc. Nicotine is depolarizing ganglionic blocker MOA: it causes an initial depolarization (↑cont.) followed by successive depolarization → transmission failure & muscular fatigue

Stimulants & their Blockers 2-Drugs act on M3 R Agonist Ach (0.01%, 0.1ml) MOA :Ach stimulate of Gs protein activate M3 R activation of PLC → Splitting of (PIP2) into: 1- DAG → ↑opening of Calcium channels 2- IP3 → mobilization of cal. from its stores ↑ intracellular calcium→contraction Antagonist Atropine (0.1ml, 0.1%) It is a specific, nonselective, reversible, competitive blocker of M3 R

Determining the site of action of unknown Blocker + Agonist Unknown Unknown Agonist Without washing