URIMEM, will facilitate biomarker discovery and change the face of medicine 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences

Advantages of Urine Proteome Non-invasive Simpler than plasma Reflects changes of plasma Close to kidney and prostate

Urine Proteome Studies

Disadvantages Big variation Need more cases to validate

Urine: may even better than blood for biomarker study Urine – Suppose to be waste – Changes were maintained if not amplified Blood – Suppose to be homeostatic – Changes were minimized

Problems More sample Higher throughput MS More analyzing power

Problems to save samples Very diluted Protein degradation

Possible ways to save Precipitate: organic solvent consuming Freeze dry: energy and labor consuming Extraction: cost Not suitable for large numbers of samples

(( Urimem

Urinary proteins dried on a filter and stored in vacuum

Why is that important?

Changed the face of medicine “Hard as it is to believe today, a single concept developed by Dr. Henry Plummer (1874 – 1936) at the beginning of the 20th century changed the face of medicine.”Dr. Henry Plummer

Changed the face of medicine The concept was a centralized medical record, stored in a single repository, and capable of traveling with the patient. (1907) Now – paperless – filmless – chartless

Biobanking: foundation of personalized medicine Blood Urine Skin cells Organ Tissue other things taken from a body Hewitt REHewitt RE. Curr Opin Oncol Jan;23(1): doi: /CCO.0b013e b8.Curr Opin Oncol.

Do we have enough? Why not? Invasiveness Storage expense Limited biological information (hair, nail)

URIMEM is the answer Invasiveness: You can’t hold your urine for a few hours. Storage expense: Proteins dried on a membrane can be stored in vacuum even at room temperature for quite a while. Limited biological information: much more complicated information from the whole body especially from the secretory system

Can we decode urine proteome? Not yet But having large number of samples will help

Acknowledgement Lulu Jia Xuejiao Liu Liu Liu Youhe Gao 高友鹤 Department of Nephrology, PUMC Hospital: Mingxi Li Funding from 973 Project