Molecular Cell, Vol. 7, 1213, 2001 Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF M.

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Molecular Cell, Vol. 7, 1213, 2001 Cdk1 Triggers Association of RNA Polymerase to Cell Cycle Promoters Only after Recruitment of the Mediator by SBF M. P. Cosma, S. Panizza, and K. Nasmyth Research Institute of Molecular Pathology Vienna, Austria

A URS1 se pega el FT Mbf (SWI5-SWI6) A URS2 se pega el FT SBF (SWI4-SWI6)

SWI5 FT SWI2 (Swi/Snf) remodel. ATP ADA2 SAGA) acetil histonas SWI4 FT Northern blot

Destino de la célula hija

ChIP-chip

The mediator coactivator complex: functional and physical roles in transcriptional regulation Brian A. Lewis and Danny Reinberg Journal of Cell Science 116, © 2003

Principales conformaciones del Mediador humano

Estructura del mediador de levadura y del complejo con RNA polimerasa II

Correspondencia entre la figura compacta y extendida del mediador

Cambios conformacionales en el Mediador humano

Posible mecanismo de acción del Mediador

Cromatinización del DNA Br Et revelado con sonda radiactiva; contra la region promotora en un caso y contra la region distal del plasmido en el otro

Posicionamiento traslacional nucleosoma

Posicionamiento rotacional (1)

Promotor de MMTV (1)

m C DNA DNMT m C bind prot HDACs Active chromatin silent chromatin Transc.fact coactivators HATs

Marcas activas Marcas represivas H3-K4 mono, di, tri H3-K9 H3-K36 H3-K27 H3-K79 H4-K20

Nco I

In vivo post viral infection Las lisinas de las histonas H3 y H4 no se acetilan al azar sino que especificamente: K8 y K12 en H4 y K9 y K14 en H3

Cell, Vol. 108, 475–487, 2002, Review: Cooperation between Complexes that Regulate Chromatin Structure and Transcription Geeta J. Narlikar, Hua-Ying Fan,and Robert E. Kingston

Modelo de como la metilacion del DNA puede estar ligada a la H4-K20me3

CDK7-ciclina H TFIIH Ser 5 CDK8-ciclina C Mediador Ser 2 y Ser 5 CDK9-ciclina T P-TEFb (positive transcrip. Elong. Factor) Ser 2 CTD humano 52 (min 28) CTD levadura 25 (min 9) CTD Drosophila 42