1. MB&B 208: Molecular Biology
Fall 2006
MWF, 10-11
Hall-Atwater 84

3. The “Central Dogma” of Molecular Biology

4. Gene definition:
Classical genetics: The functional unit of inheritance.
Molecular genetics: The nucleic acid sequence that is necessary for the synthesis of a functional polypeptide (protein) or RNA.

11. Transcription initiation in eukaryotes
RNA polymerase: CTD = carboxy terminal domain
GTF’s = general transcription factors
TFIID: includes TBP, binds TATA box
TFIIH: helicase and CTD kinase
Initiation: phosphorylation of CTD by TFIIH
GTF’s do not remain associated with transcribing
polymerase

12. TBP (TATA binding protein)

16. Gene transcription: role of transcription factors
1. Recruitment of RNA polymerase
2. Overcome inhibition by chromatin structure