Transcriptomic responses of Emiliania huxleyi to Ocean Acidification Sebastian D. Rokitta, Uwe John and Björn Rost.

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Transcriptomic responses of Emiliania huxleyi to Ocean Acidification Sebastian D. Rokitta, Uwe John and Björn Rost

Ocean Acidifcation CO 2 pH DIC Year [CO 3 2- ] pH SWS [DIC] [µmol kg -1 ] CO 3 2- [CO 2 ] [µmol kg -1 ] After Wolf-Gladrow et al. (1999)

Photo: NASA, PML Coccolithophores

Biological carbon pumps

OA-responses in E. huxleyi Modified from Hoppe et al. (2011)

Energization?

The matrix approach Light vs. pCO 2 50 µmol photons m -2 s µmol photons m -2 s µatm 1000 µatm Acclimation data (µ, POC, PIC) Physiology (C i acquisition, light reactions) Transcriptomics (gene expression)

Phenomenology Rokitta & Rost (2012) PIC production drops (especially under low light!) POC production is boosted (especially under low light!) TPC production is insensitive

Physiology Rokitta & Rost (2012) More POC despite less pigmentation and O 2 evolution  Improved energy efficiency under OA

Gene expression?

Transcriptomics Low-light acclimation 1172 ↑ 861 ↓ High-light acclimation 1082 ↑ 814 ↓ OA responsive genes

Low-light specific 447 ↑ 236 ↓ High-light specific 357 ↑ 189 ↓ 725 ↑ 625 ↓ Core OA-response Transcriptomics

# of genes; sign denotes regulation (+ ↑ ; - ↓ ) Carbon metabolism Light physiology Signalling Ion fluxes Core OA-response LL specific OA-response HL specific OA-response Organellar shuttling ↑ Pentose phosphate pathway ↑ Glycolysis ↓ Fatty Acid & Glucan anabolism ↑ Energy dissipation ↑ Lipid and IP 3 signaling ↑ Membrane potentials ↑ Regulation of C fluxes ↑ Energy dissipation ↑ Transcriptomics

OA re-wires carbon fluxes NADPHNADH

OA affects the redox hub Carbon metabolism Trx XC Asc GSH Chloro- plast EMS NADP/NADPH NAD/NADH... Mitochon- drium

Conclusions OA causes a shunting of carbon from calcification towards biomass production OA-Responses are modulated by energy availability and typically attenuated by high light OA affects cellular signaling and the redox hub and thereby re-wires carbon flux networks