Restriction Enzymes DNA is a long chain of nucleotides. AluI recognises AGCT, and fixes itself on its target sequence. It cuts after the G to give Blunt.

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Presentation transcript:

Restriction Enzymes DNA is a long chain of nucleotides. AluI recognises AGCT, and fixes itself on its target sequence. It cuts after the G to give Blunt Ends AGCT TCGA

Restriction Enzymes DNA is a long chain of nucleotides. AluI recognises AGCT, and fixes itself on its target sequence. It cuts after the G to give Blunt Ends AG CT TC GA AGCT TCGA

Restriction Enzymes EcoRI recognises GAATTC, and fixes itself on its target sequence. It cuts after the G to give Sticky Ends GAATTC CTTAAG

Restriction Enzymes EcoRI recognises GAATTC, and fixes itself on its target sequence. It cuts after the G to give Sticky Ends GAATTC CTTAAG G AATTC CTTAA G

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