Pre-exam Tutorial Anthony P.F. Turner Biosensors and Bioelectronics Centre

2 Course Syllabus This course will provide a concise overview of biosensor technology and its application in healthcare, food safety, environmental monitoring and security. Following general introductions highlighting the economic importance and multidisciplinary nature of this rapidly expanding field and the broad applicability of this technology, the lectures and practical exercises will focus on the construction, design and manufacture of biosensors, the principal and emerging materials and components used and three case studies of key devices.

3 Course Syllabus II The course will give insights into the complexities involved in combining biological materials such as enzymes, antibodies and DNA with optical, electrochemical and other transducers to provide practical and easy-to-use systems. In order to integrate this knowledge and to introduce the importance of intellectual property protection, lectures and a group project will then examine some pivotal biosensor patents and an example of patent litigation.

4 Course Syllabus III The course will conclude with a consideration of commercialisation routes, ethical issues and future perspectives. The overall target of the course is to give the student a thorough understanding of the fundamentals and applications of biosensor technology together with an appreciation of its current and future impact on society. Following the course, the student should be able to design and construct a simple biosensor, know how to operate a biosensor in the laboratory and have an understanding of the manufacture, commercialisation and use of biosensors for a variety of applications in the real world

Answering Exam Questions You have 4 hours and 8 questions; spend 30 minutes on each question READ THE QUESTION CAREFULLY Answer EVERY element of the question (don’t throw away marks!) Look out for guidance in the question as to how to answer (e.g. ”briefly”; ”in detail”, ”illustrate” or ”draw”; ”give an/2/3 example(s); ”compare” or ”show difference”; ”discuss” Draw diagrams, give equations and detail chemical reactions at every opportunity 5

6 Lectures 1)Introduction to Biosensors (Anthony Turner) 2)Applications of biosensors (Martin Mak) 3)Transducers and sensor systems (Valerio Beni) 4)Bioreceptors and immobilisation (Martin Mak) 5)Synthetic receptors and nanomaterials (Ashutosh Tiwari) 6)Design parameters for catalytic biosensors (Valerio Beni) 7)Design parameters for affinity biosensors (Martin Mak)

7 Lectures II 8)Biosensor design (Martin Mak) 9)Surface Plasmon Resonance (Karin Enander) 10)Glucose biosensors for Diabetes (Anthony Turner) 11)Biomimetic systems (Fredrik Winquist) 12)Microfluidics and arrays (Edwin Jager) 13)Patenting and litigation in the field of biosensors (Anthony Turner) 14)Commercialising biosensors, ethics and future prospects (Anthony Turner)

8 Practicals, Demonstrations & Group Project Enzyme Electrodes Surface Plasmon Resonance Printed Biosensors & Microfabrication Biacore SPR Patent – Integrating exercise

Good luck! Questions? 9