1. Engineering receptors and antibodies for biosensors Author: B. Hock, M. Seifert, K. Kramer Presented by: Abu Bakar Md. ISMAIL & Balakrishnan RAMANATHAN

2. Objectives: 1. Effect monitoring with biological components as targets for bioeffective substances. 2. Immunochemical analysis employing anibodies as binding protiens.

3. Experimental Tool Surface Plasmon Resonance (SPR) sensing principle

4. Experiments Experiment 1: Characterization of receptors. Experiment 2: K inetic and affinity measurements of recombinant antibody fragments derived from antibody libraries with s-triazine selectivities.

5. Experiment 1…….. Estrogen Receptor: Expression & Purification Expression of the ER  in yeast (16 kDa)

6. Experiment 1…….. Immobilization of Binding of ER  17ß-estradiol-BSA Estrogen Receptor: Expression & Purification Binding studies with the recombinant ER:

7. Experiment 1………. Response for increasing Calibration curve for 17β-estradiol concentration Competitive receptor assay (b) Competitive receptor assay in biosensor format:

8. 7 minutes………...? Ooops…………... That’s all from me …………………………...