Digesting macromolecules lab

Outline of the day 1.Turn in your lab reports at the front –More than 10 minutes late = bad 2.Any questions on last week’s lab? 3.Quiz 4.Introduction to the lab 5.Lab! 6.Check out Get a stamp Make sure I mark you down for attendance

Quiz Ends 10 minutes after it’s started –Ends at: ____

Lab this week! Exploring digestion! –How carbohydrates, proteins, and fats are digested –Effect of enzyme inhibitors on carbohydrate digestion –Effect of pH on protein digestion –Requirements for fat digestion

Why do we eat?

What are we doing when we eat? Digestion: Dissolving food and breaking it down into smaller molecules Absorption: The entry of molecules across the gut epithelium into the body

What types of macromolecules are there? How do we digest them?

Digesting food Dozens of enzymes help during digestion –Enzymes make reactions go faster (and be more likely to occur) Missing image: Image showing enzymes reducing the energy require for a reaction to progress.

Today we’re going to see what effects the digestion of macromolecules Using indicators to test for various steps of digestion –E.g., Iodine changes color in the presence of starch Blue/black = starch present Brown = no starch present

Detecting the breakdown of starch with iodine Missing image: IKI test samples

Detecting the breakdown of starch by testing for the presence of sugar Missing image: Benedict’s test

Detecting the breakdown of protein into amino acids with egg whites Missing image: Egg white test

Detecting the breakdown of fat into glycerol and fatty acids Missing text: Description of lipid test

Before you leave Clean up your work area –Wash glassware and store upside down Show me your lab report so I can stamp it –Need to have all data fields filled in –Complete at home and then turn in at the beginning of next lab Remember that we’ll have a quiz at the beginning of the next class –6-7 questions on today’s lab –3-4 questions on the lab we’ll do next week

Notes for the instructor: Add any relevant cleanup instructions to the final slide (that slide is a generic one I’m adding to each presentation). The last few slides (on each biochemical test) were based on excellent slides from Kelli Elliott (of Orange Coast College); contact her for the images and other text that go along with them. Note also that the first few slides of the presentation are designed to be used to facilitate class discussion (I create lists of the answers to each question on the board as the class provides them)

License information This work is licensed under the Creative Commons Attribution- NonCommercial-ShareAlike 3.0 License. To view a copy of this license, visit sa/3.0/us/ or send a letter to Creative Commons, 171 Second Street, Suite 300, San Francisco, California, 94105, USA. The slides in this presentation were originally created by Marc C. Perkins ( You are free to use, modify, and distribute these slides according to the terms of the Creative Commons license (e.g., you must attribute the slides, no commercial uses are allowed, and future distributions must be licensed under a similar license). Attribution should be given to Marc C. Perkins (and any later editors), including a link back to Marc’s current website. This applies both while distributing the slides and during use of the slides; attribution during use can be satisfied by, for instance, placing small text on at least one of the slides that has been shown (see below for an example). Slides in this presentation based on those created by Marc C. Perkins.

History August 2007: Marc Perkins released first version. (If you modify these slides and redistribute them, add your information to the list)