Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo.

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Presentation transcript:

Identifying Sources of Microbial Pollution Using Traditional Microbial and Molecular Source Tracking Techniques Julie Gaertner University of Hawai`i Hilo M.S. Tropical Conservation Biology & Environmental Science

Microbial Methods Clostridium perfringens Enterococcus spp. Enumerated with Enterolert Kits by IDEXX Laboratories Inc. Enumerated by Membrane Filtration Method Bisson and Cabelli Photo Credit: Santuarysimon.org Photo Credit: Oxiod.com

Molecular Method Human Specific Genetic Markers Bacteroidales 16S rRNA Bacteroides-Prevotella group Length heterogeneity PCR Terminal restriction fragment length polymorphism (T-RFLP) PCR detection of human-specific Bacteroides genetic marker from sewage (Bower et al. 2005).

Water Quality Parameters Total Nitrogen Total Dissolved Nitrogen Nitrate + Nitrite Ammonium Total Phosphorus Phosphate Light Levels Salinity Temperature Specific Conductivity Dissolved Oxygen Turbidity pH Weather & Climate Data

High Resolution GIS Mapping CYCLOPS-7 Optical Brightener Fluorometer Probe DataBank Data Logger – GPS Enabled Map Credit: Ambyr-Mokiao-Lee Photo Credit: Turnerdesigns.com

Acknowledgements Dr. Tracy Wiegner Dr. Jon Awaya Dr. Stephanie Molloy Dr. Misaki Takabayashi Dr. Mazen Hamad Ambyr Mokiao-Lee Eric Johnson Lucas Mead Narrissa Spies Steven Bradshaw University of Hawai`i Hilo EPSCoR Analytical Hawai`i Department of Health

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