1. Examination of Cyanobacteria (Blue-Green Algae) Microcystin- LR Toxin in Callander Bay Presented by: Jamie Lavigne Supervised by: Dr. Reehan Mirza Department of Biology and Chemistry Nipissing University - 2012

2. Outline Callander Bay Blue-green Algae Objectives Future work

3. Callander Bay Historically ◦ Logging, sawmill operations, agricultural activities, rural and urban development (Hutchinson Environmental Sciences Ltd. 2011). Photos accessed from http://www.museevirtuel.ca/pm.php?id=story_line&lg=Francais&fl=0&ex=92&sl=2929&pos=1&pf=1#8

4. Callander Bay Growing concern over the appearance of harmful blue-green algal blooms Municipal drinking water Recreational use Attracts tourists/anglers worldwide

5. Objectives Compare pre, during and post bloom event 1. Measure water quality parameters  pH, temperature, dissolved oxygen, conductivity, water hardness 2. Identify zooplankton 3. Analyze microcystin-LR in water and fish tissue.

6. BGA Species identified in 2011 Anatoxins 1.Anabaena spp. 2.Ahanizomenon flos- aquae Microcystins 1.Anabaena spp. 2.Gloeotrichia echinulata 3.Microcystis sp. 4.Woronichinia sp. 5.Limnothrix sp.

7. Sampling Sites Photo courtesy of the North Bay-Mattawa Conservation Authority

8. Water Quality – Dissolved Oxygen

9. Water Quality – Water Hardness

10. Water Quality - Conductivity

11. Water Quality - pH

12. Water Quality - Temperature

14. YSI – BGA Sensor

15. BGA Cell Count

16. Zooplankton Population Size

17. Microcystin-LR Analysis

18. Future Work MESc. ◦ Project will continue for 2 years ◦ Continue to collect water, fish tissue and zooplankton ◦ Expand sampling area, longer timeframe ◦ Use of BGA sensor (cells/mL) ◦ Develop a method to detect microcystin

19. Acknowledgements Thank you to the following: ◦ Nipissing University ◦ Township of Callander ◦ Callander Sustainable Committee ◦ North Bay-Mattawa Conservation Authority ◦ North Bay-Parry Sound District Health Unit ◦ Ministry of Environment