Macaw #315, female. GGA1 AMA1q,4,9q Chicken control.

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Presentation transcript:

Macaw #315, female

GGA1 AMA1q,4,9q Chicken control

GGA2 AMA2 Chicken control Red – GGA11 BAC pool GGA11 GGA11 – AMAZ!!!

GGA3 AMA3 Chicken control

Red – GGA11 BAC pool GGA4 AMA1p, 8q GGA11 GGA11 – AMAZ!!!

GGA5 AMA5q Red: BAC P1H3 on GGA5 No signal on macaw Chicken control

GGA6 AMA6 segments Chicken control Red: FZF – GGA6

GGA7 AMA6q Chicken control Red: FZF – GGA6

CHICKEN CONTROL Paint for GGA6 Paint for GGA7 BAC clone for GGA6 Macaw-GGA6 paint and BAC Macaw-GGA7 paint and BAC Macaw-GGA6 and 7 paints and BAC AMA6 GGA6 paint GGA7 paint GGA6 and GGA7 are Syntenic in macaw Chicken GGA6 BAC clone (CHY4 gene) gives clear signal in macaw!!!

GGA8 AMA7p,7qter and micros Chicken control

GGA9 AMA7qmid

GGAZ AMAZ W Chicken control (female) W Male- Erica Female-#315

GGAW No signal Chicken control (female) No signal on macaw W chromosome – similar situation is with mammalian Y which, as a rule, does not give Zoo-FISH signal across diverged species.

5.8S, 18S, 28S rDNA Chicken control – 1 NOR 3 pairs of microchromosomes with NORs (Chicken and California condor have only one NOR chromosome)

Telomeres Macaw has telomeres at chromosome ends only - no interstitial telomeric sites. Prominent number of Telomeric sequences are in microchromosomes Macaw #315 W W Z Z