Bacterial Transformation Teacher Preparation Materials Lists.

Slides:



Advertisements
Similar presentations
Microbiological Methods
Advertisements

API 20e Dr.ayham abulaila.
Transformation Lab Student Instructions. Student Instructions Step 1 Remove two tubes from the ice bucket. Label one B1 and the second "B2." Transformation.
Microbiological Methods
Lab 5/5a Transformation of E. coli with a Recombinant Plasmid
PHT 381 Lab # 9. MIC: It is the lowest concentration of the antimicrobial agent that inhibits the growth of the test organism but not necessarily kills.
Media Prep. & Model Organisms Lab 4E, 4F & 2B. Timeline/Overview Monday: Lecture Tuesday: Media Prep (4E & 4F) Pour Plates for Day 2 of Lab 2B Thursday:
PHT 381 Lab # 9. MIC : - It is the lowest concentration of the antimicrobial agent that inhibits the growth of the test organism but not necessarily kills.
pGLO Transformation LAB AP LAB 6 BIO-RAD lab book pGLO ori bla GFP araC.
Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms.
Transformation of Escherichia coli
Bacterial Transformation with ( pGLO Plasmid) Lab #9: Molecular Biology.
Determination of MIC by Agar Diffusion Method. Minimum Inhibitory Concentration (MIC)  Definition: is the lowest concentration of an antimicrobial agent.
ASEPTIC TECHNIQUE Removing inoculum from a broth culture
Recombinant DNA Bacterial Transformation Student Instructions Transformation.
Bacterial Transformation
Bacterial identification procedure Part 1 : Preparation of API miniaturized system.
Ex. 12: Chemical Antimicrobial Agents: Antibiotics Objectives ??
Bacterial Transformation Teacher Preparation
Microbiological Methods
Plasmid DNA Restriction Enzymes “cut” Plasmid DNA Piece of DNA is Removed New Piece (gene) of DNA is “stitched” to Plasmid DNA New DNA (gene)
Learning Targets “I Can...” -Explain what it means for a gene to be expressed. -Explain the role of plasmids. -Insert a plasmid into bacteria to observe.
The Characteristics of Model Organisms
Aseptic Technique: Media and Equipment. Growth Medium A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms.
Transformation with Firefly gene Lab Protocol
Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.
Lab 18 Goals and Objectives: Exercise 21: Enumeration Work as two pairs per group (max 5 groups) Both pairs use same Escherichia coli culture Use pre-poured.
The Effects of Chlorine on Harmful Bacteria Group Leow Shawn Tao (1A413, Leader), Sow Jeng Wei (1O220), Justin Soh (1P111), Soh Yee Kiat (1P127)
Content Standard 1 Select appropriate laboratory glassware, balances, time measuring equipment, and optical instruments to conduct an experiment.
Laboratory Procedure for bacterial transformation with pGLO It’s glowing.
Recombinant DNA Bacterial Transformation Student Instructions Mid-Log Culture Prep.
Bacterial Transformation Teacher Preparation Sterile Materials.
Pre Lab Definitions: (Fill these in on your lab paper) Serial: In a series, order or interval. Measured steps. Dilution: Water Down. Pipette: “Little pipe”
Genetic Engineering BSC 1010L Transformation of E. coli with Jellyfish GFP.
Recombinant DNA Bacterial Transformation Teacher Preparation Sterile Materials.
Lab 8Biology You will serially dilute a known mass of soil, make agar plates of the dilutions and estimate the number of microorganisms in the original.
Determination of spectrum of activity of antibiotics.
Bacterial Transformation Lab
By: Lynn More - Olympian High School and UCSD Protein Transformation Lab Preview UCSD: BioBridge Program.
Recombinant DNA Bacterial Transformation Student Instructions Making Competent Cells.
Laboratory: Bacterial Transformation
PGLO Transformation LAB AP LAB 6 BIO-RAD lab book pGLO ori bla GFP araC.
Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)
Microbiological Methods
Plasmid Transformation Lab
AP Biology BACTERIAL TRANSFORMATION LAB. Lab Procedures, Rationale, and Protocol Please read the pdf file with the full lab description that can be found.
Strain collection Who are we? Faqs
Bacterial Cultures *Bacteria grow best in warm, moist, dark areas that contain a lot of food. -When we culture bacteria, we provide them with this environment.
By: 1. Teo Boon Boon 2. Khor Mei Ann 3. Ooi Shu YU.
Culturing Bacteria Growth Media
Bacterial Transformation with (pGLO Plasmid)
Life in Bloom Day 1 Experiment set up.
Marine Biotechnology Lab
Take out a sheet of paper and label with the heading on this slide.
Pre-Lab: pGLO Bacterial Transformation
Bacterial Transformation Visual Guide
Do Now What is E.coli? And why is it used for biotech?
PLASMID ISOLATION AND ANALYSIS Part I Overnight Culture Suspension
Lab 8 Warm-Up What are plasmids?
Lab 8 Warm-Up What are plasmids?
Exercise 21: Enumeration Work as two pairs per group (max 5 groups)
Protein Transformation Prep
By: Lynn More - Olympian High School and UCSD
Transformation of Escherichia coli
Bacteria Inquiry.
Transformation of Escherichia coli
Transformation of Escherichia coli
Transformation of Escherichia coli
Presentation transcript:

Bacterial Transformation Teacher Preparation Materials Lists

Materials List-Item 1 (per group of students) 1 aluminum foil packet containing 4 sterile paper clips that are large and smooth. The clips should be opened into a 90° angle and the small end bent to close it. Bacterial Transformation Teacher Preparation

Materials List -Item 2 (per group of students) 1 aluminum foil packet containing 4 sterile paper clips that are small and smooth. Bend one end of each paper clip into a loop. Bacterial Transformation Teacher Preparation

Materials List -Item 3 (per group of students) 2 microcentrifuge tubes (1.5 ml) containing 2 drops of sterile CaCl 2 and labeled "CaCl 2 " Place on ice in a refrigerator until ready to use. Bacterial Transformation Teacher Preparation

Materials List -Item 4 (per group of students) 1 petri dish containing only nutrient agar and 1 dish with ampicillin labeled "No Amp" on the bottom Bacterial Transformation Teacher Preparation

Materials List -Item 5 (per group of students) 1 petri dish containing nutrient agar and 1 dish with antibiotic ampicillin labeled "Amp" on the bottom Bacterial Transformation Teacher Preparation

Materials List -Item 6 (per group of students) Copies of the laboratory instructions, one for each student Bacterial Transformation Teacher Preparation

Materials List -Item 7 (per group of students) 1 glass bottle with a cap containing sterile nutrient broth and labeled "LB Broth" Bacterial Transformation Teacher Preparation

Materials List -Items 8 & 9 (per group of students) 1 Sharpie marking pen 1 rubber bulb for the pipettes Bacterial Transformation Teacher Preparation

Materials List -Item 10 (per group of students) 1 aluminum foil packet containing the glass parts of 4 sterile pasteur pipettes or sterile plastic pipettes Bacterial Transformation Teacher Preparation

Materials List -Item 11 (per group of students) 1 aluminum foil packet containing 8 sterile toothpicks Bacterial Transformation Teacher Preparation

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2024 SlidePlayer.com Inc. All rights reserved.