Fotoinhibice fotosyntézy fluorometrické metody stanovení M. Barták Metody fluorescence chlorofylu Jaro 2014.

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Fotoinhibice fotosyntézy fluorometrické metody stanovení M. Barták Metody fluorescence chlorofylu Jaro 2014

Basic de-excitation mechanisms of Chl a Chl a* Photosynth.process (linear transport e- ) Thermal dissipation Regulation between PSII and PSI Chlorophyll fluorescence

Reversible transport Q a Q a 3 (P680 + Pheo - ) 1 (P680 + Pheo - ) 3 (P680 + Pheo - ) 1 (P680 + Pheo - ) 3 P680* 1 P680* 3 P680* 1 P680* 3  carotene* P 680 Fluorescence O 2 * O 2 * 3  carotene* 3 Chla* Chla* heat Singlet Recombin. Charge separation Triplet Recomb. Antennae q. Photosynthetic pathway

Metody stanovení míry fotoinhibice Kautského křivka fluorescence chlorofylu doplněná o zhášecí mechanismy Kautského křivka fluorescence chlorofylu doplněná o zhášecí mechanismy Časové řady parametrů fluorescence chlorofylu Časové řady parametrů fluorescence chlorofylu

qP = (Fm’- F) / (Fm’-Fo’) NPQ = (Fm - Fm’) / (Fm’) qN = qE + qT + qI qE

qT = Fm’ after rapid relaxation is complete usually with the actinic light turned off usually one hour - Fm’ at qE /Fm’ at steady state. qI = Fm-Fm’ at qT/ Fm’ at steady state. qN = Fm - Fm’/ Fm-Fo qL = qP(Fo’/F’) Y(NO) = 1/NPQ +1 + qL((Fm/Fo)-1) Y(NPQ) = 1 - Y - Y(NO) 1 = qL + Y(NPQ) + Y(NO) qP = (Fm’- F) / (Fm’-Fo’) NPQ = (Fm - Fm’) / (Fm’) qN = qE + qT + qI qE

tokyo.ac.jp/users/seitaipl/personal/terashima/ter ashima_e.html

Brno, Laboratoř fotosyntetických procesů Senzitvita vůči dlouhotrvající fotoinhibici A zotavení se v